Increased placental sFlt-1 but unchanged PlGF expression in late-onset preeclampsia

Ehrlich, Laura; Hoeller, Alice; Golić, Michaela; Herse, Florian; Perschel, F. H.; Henrich, Wolfgang; Dechend, Ralf; Huppertz, Berthold; Verlohren, Stefan

doi:10.1080/10641955.2017.1291673

Cited by 15 publications

(6 citation statements)

References 26 publications

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“…Finally, we did not evaluate the release of this protein from the placenta into the maternal circulation for future use of predictive estimates with simple blood tests. Huppertz et al [ 45 ] have demonstrated the importance of obtaining results of a placental protein from diversified origins (placenta, serum, amniotic fluid) to reach a comprehensive understanding of the actual origin of the measured protein and further verify its role in the placental pathology in PE [ 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Reduced Placental CD24 in Preterm Preeclampsia Is an Indicator for a Failure of Immune Tolerance

Sammar

Siwetz

Meiri³

et al. 2021

IJMS

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Introduction: CD24 is a mucin-like glycoprotein expressed at the surface of hematopoietic and tumor cells and was recently shown to be expressed in the first trimester placenta. As it was postulated as an immune suppressor, CD24 may contribute to maternal immune tolerance to the growing fetus. Preeclampsia (PE), a major pregnancy complication, is linked to reduced immune tolerance. Here, we explored the expression of CD24 in PE placenta in preterm and term cases. Methods: Placentas were derived from first and early second trimester social terminations (N = 43), and third trimester normal term delivery (N = 67), preterm PE (N = 18), and preterm delivery (PTD) (N = 6). CD24 expression was determined by quantitative polymerase chain reaction (qPCR) and Western blotting. A smaller cohort included 3–5 subjects each of term and early PE, and term and preterm delivery controls analyzed by immunohistochemistry. Results: A higher expression (2.27-fold) of CD24 mRNA was determined in the normal term delivery compared to first and early second trimester cases. The mRNA of preterm PE cases was only higher by 1.31-fold compared to first and early second trimester, while in the age-matched PTD group had a fold increase of 5.72, four times higher compared to preterm PE. The delta cycle threshold (ΔCt) of CD24 mRNA expression in the preterm PE group was inversely correlated with gestational age (r = 0.737) and fetal size (r = 0.623), while correlation of any other group with these parameters was negligible. Western blot analysis revealed that the presence of CD24 protein in placental lysate of preterm PE was significantly reduced compared to term delivery controls (p = 0.026). In immunohistochemistry, there was a reduction of CD24 staining in villous trophoblast in preterm PE cases compared to gestational age-matched PTD cases (p = 0.042). Staining of PE cases at term was approximately twice higher compared to preterm PE cases (p = 0.025) but not different from normal term delivery controls. Conclusion: While higher CD24 mRNA expression levels were determined for normal term delivery compared to earlier pregnancy stages, this expression level was found to be lower in preterm PE cases, and could be said to be linked to reduced immune tolerance in preeclampsia.

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Section: Discussionmentioning

confidence: 99%

Reduced Placental CD24 in Preterm Preeclampsia Is an Indicator for a Failure of Immune Tolerance

Sammar

Siwetz

Meiri³

et al. 2021

IJMS

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“…28,29 Total mRNA was Women were divided into four subgroups: early-onset PE (n = 7), lateonset PE (n = 18), FGR (n = 13), and a control group (n = 16).…”

Section: B7-h4 Mrna Expression In Placental Villous Tissue -Study Pmentioning

confidence: 99%

Soluble B7‐H4 blood serum levels are elevated in women at high risk for preeclampsia in the first trimester, as well as in patients with confirmed preeclampsia

Mach

Nolte-Boenigk

Droste

et al. 2018

American J Rep Immunol

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“…The explanation could be related to PLGF decrease with labor duration;48 PLGF (both mRNA and protein) has also been observed as decreasing in labor samples 48. Most such studies have been carried out in maternal serum 25,49,50…”

Section: Discussionmentioning

confidence: 99%

The umbilical cord, preeclampsia and the VEGF family

Contreras

Garrido

Hernández-Losa

et al. 2018

IJWH

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IntroductionThe VEGF family has been identified as abnormal in preeclampsia (PE). Hypertensive disorders of pregnancy (HDP) are major contributors to maternal and neonatal morbidity and mortality worldwide; likewise, umbilical cord anatomical abnormalities (UCAA) are linked to poor neonatal outcomes. Based on the relationship described between PE and UCAA and the role of the VEGF family in PE, this study explored VEGF expression in placental and UC tissued from patients with PE and with UCAA.MethodsWe performed an observational, analytical study on placentas, comparing protein and mRNA expression in four groups: patients with PE, patients with UC abnormalities, patients with both, and patients with none of them. Using immunohistochemistry, we studied VEGF A, VEGF R1 (FLT1), MMP1, and PLGF. With quantitative reverse transcription polymerase chain reaction we described mRNA expression of PLGF, VEGF and sFLT1, and sFLT1/PLGF ratio.ResultsForty newborns were included. Sixty-seven percent of mothers and 45% of newborns developed no complications. Immunohistochemistry was performed on UC and placental disc paraffin-embedded tissue; in the latter, the mRNA of the VEGF family was also measured. Statistically significant differences were observed among different expressions in both HDP and UCAA groups. Interestingly, the UCAA group exhibited lower levels of sFLT1 and VEGF-A in comparison with other groups, with significant P-value for sFLT1 (P=0000.1).ConclusionThe origin of UCAA abnormalities and their relation with HDP are still unknown. VEGF family alterations could be involved in both. This study provides the first approach related to molecules linked to UCAA.

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Increased placental sFlt-1 but unchanged PlGF expression in late-onset preeclampsia

Abstract: High sFlt-1 concentrations may account for diminished maternal serum PlGF levels.

Cited by 15 publications

References 26 publications

Reduced Placental CD24 in Preterm Preeclampsia Is an Indicator for a Failure of Immune Tolerance

Reduced Placental CD24 in Preterm Preeclampsia Is an Indicator for a Failure of Immune Tolerance

Soluble B7‐H4 blood serum levels are elevated in women at high risk for preeclampsia in the first trimester, as well as in patients with confirmed preeclampsia

The umbilical cord, preeclampsia and the VEGF family

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