Increased production of biofilms by Escherichia coli in the presence of enrofloxacin

Costa, Jorge de Assis; Espeschit, Isis de Freitas; Pieri, Fábio Alessandro; Benjamin, Laércio dos Anjos; Moreira, Maria Aparecida Scatamburlo

doi:10.1016/j.vetmic.2012.05.036

Cited by 31 publications

(32 citation statements)

References 14 publications

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“…Previous studies showed that subinhibitory concentrations of gentamicin (aminoglycoside) and enrofloxacin (quinolone), both used in the treatment of clinical mastitis, induced the formation of E. coli biofilms (9,12). Therefore, these results, together with the data in this study, suggest that antimicrobials present at sub-MICs can significantly induce in vitro biofilm formation by E. coli obtained from mastitic milk.…”

Section: Resultssupporting

confidence: 79%

“…Furthermore, some antimicrobial agents can stimulate the production of biofilms by microorganisms (9)(10)(11). Subinhibitory concentrations of aminoglycosides, such as gentamicin, tobramycin, and amikacin, induce biofilm production in Pseudomonas aeruginosa and E. coli isolates from patients with cystic fibrosis (12).…”

mentioning

confidence: 99%

“…Subinhibitory concentrations of aminoglycosides, such as gentamicin, tobramycin, and amikacin, induce biofilm production in Pseudomonas aeruginosa and E. coli isolates from patients with cystic fibrosis (12). Antimicrobials used in the treatment of clinical mastitis, such as gentamicin (aminoglycoside) and enrofloxacin (quinolone), at subinhibitory concentrations induce biofilm formation by E. coli isolates from cases of mastitis (9). This might have clinical relevance, as bacteria are exposed to sub-MICs of antimicrobials at the beginning and end of a dosing regimen (between doses) or continuously during low-dose therapy (13).…”

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confidence: 99%

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Biofilm Formation on Biotic and Abiotic Surfaces in the Presence of Antimicrobials by Escherichia coli Isolates from Cases of Bovine Mastitis

Silva

Soares

Júnior

et al. 2014

Appl Environ Microbiol

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Escherichia coli is a highly adaptive microorganism, and its ability to form biofilms under certain conditions can be critical for antimicrobial resistance. The adhesion of four E. coli isolates from bovine mastitis to bovine mammary alveolar (MAC-T) cells, biofilm production on a polystyrene surface, and the expression profiles of the genes fliC, csgA, fimA, and luxS in the presence of enrofloxacin, gentamicin, co-trimoxazole, and ampicillin at half of the MIC were investigated. Increased adhesion of E. coli isolates in the presence of antimicrobials was not observed; however, increased internalization of some isolates was observed by confocal microscopy. All of the antimicrobials induced the formation of biofilms by at least one isolate, whereas enrofloxacin and co-trimoxazole decreased biofilm formation by at least one isolate. Quantitative PCR analysis revealed that all four genes were differentially expressed when bacteria were exposed to subinhibitory concentrations of antimicrobials, with expression altered on the order of 1.5-to 22-fold. However, it was not possible to associate gene expression with induction or reduction of biofilm formation in the presence of the antimicrobials. Taken together, the results demonstrate that antimicrobials could induce biofilm formation by some isolates, in addition to inducing MAC-T cell invasion, a situation that might occur in vivo, potentially resulting in a bacterial reservoir in the udder, which might explain some cases of persistent mastitis in herds.

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Section: Resultssupporting

confidence: 79%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Biofilm Formation on Biotic and Abiotic Surfaces in the Presence of Antimicrobials by Escherichia coli Isolates from Cases of Bovine Mastitis

Silva

Soares

Júnior

et al. 2014

Appl Environ Microbiol

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“…Previous studies show that subinhibitory concentrations of antibiotics can trigger the formation of biofilms (5,6). Pathogenic bacteria are embedded in a self-produced extracellular protein matrix and some micromolecules, including exopolysaccharide (EPS) and DNA (7,8).…”

Section: Introductionmentioning

confidence: 99%

Biofilm-Producing Ability of Bovine Extraintestinal Pathogenic Escherichia coli and Its Correlation with Attachment Factors

Bakhtiari

Gooraninezhad

Karami

2018

Jundishapur J Health Sci

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Background: Escherichia coli is recognized as a common cause of infection. Long-lasting presence of bacteria on biotic and abiotic surfaces and failure of bacterial eradication are predicted by biofilm production. Some extracellular frills of E. coli can be implicated in productive events, leading to biofilm formation by surface colonization. Objectives: In the present study, correlation of csgA (encoding curli fimbriae) and fimA (encoding a large subunit of type I fimberiae) gene expression with biofilm formation of extraintestinal bovine pathogenic E. coli strains was evaluated in different enrichment media. Methods: The microtiter plate-based crystal violet method was applied to examine the biofilm production of 30 E. coli strains in Luria-Bertani (LB) and Brain-Heart Infusion (BHI) broth with 1% sucrose. PCR assay was performed to determine the presence of the studied genes. Results: According to the results, 100% of isolates contained csgA genes, and 96.7% contained fimA genes. Using the BHI medium with 1% sucrose, 53.3% and 16.6% of strains were average and strong biofilm producers, respectively. On the other hand, by using the LB medium, 66.6% of isolates were poor biofilm producers, whereas none were strong biofilm producers. Conclusions:The BHI medium containing 1% sucrose was better detected in biofilm production, compared to the LB medium. Since the studied genes were present in non-biofilm producing isolates, the correlation of these genes with biofilm-producing ability is questioned.

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“…Antimicrobials are not only hindered by the biofilm matrix in their action against the microorganisms embedded in it, they may also induce the formation of biofilms dependant on the bacterial species and the antimicrobial concentration (Kaplan, 2011;Costa et al, 2012). The application of antimicrobials over a liquid feeding system therefore poses a risk of selecting antimicrobial resistant bacteria (ARB) in the biofilm by locally sub-inhibitory antimicrobial concentrations and the favourable conditions in the biofilm matrix for horizontal gene transfer (HGT) (Flemming and Wingender, 2010).…”

Section: Introductionmentioning

confidence: 99%

The effect of the administration of three different antimicrobial premix formulations via the liquid feeding system on the occurrence of Enterobacteriaceae resistant to tetracycline in the liquid feed for pigs

Heller

Sidler

Hässig

et al. 2016

SAT

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The oral group treatment is still a common procedure in swine production. This project studied the effect of the application of 3 different formulations of antimicrobial premixes (1. chlortetracycline, 2. chlortetracycline + sulfadimidine + tylosin, 3. sulfadimidine + sulfathiazole + trimethoprim) via the liquid feeding system on the occurrence of tetracycline-resistant Enterobacteriaceae (Ent-Tetr) in the liquid feed. 156 and 112 feed samples were collected between April and December 2015 in 13 case and 14 control farms, respectively. The 27 farms were randomly selected pig fattening farms located in different parts of Switzerland. The number of feed samples that contained Ent-Tetr as well as the number of Enterobacteriaceae resistant to tetracycline per sample was significantly higher in the case group than in the control group. The use of any of the 3 antimicrobial combinations turned out to be the main risk factor for the occurrence of Ent-Tetr in the liquid feed. Our results suggest that liquid feed containing antimicrobials is a reservoir of antimicrobial resistant bacteria in swine production. AbstractThe oral group treatment is still a common procedure in swine production. This project studied the effect of the application of 3 different formulations of antimicrobial premixes (1. chlortetracycline, 2. chlortetracycline + sulfadimidine + tylosin, 3. sulfadimidine + sulfathiazole + trimethoprim) via the liquid feeding system on the occurrence of tetracycline-resistant Enterobacteriaceae (Ent-Tetr) in the liquid feed. 156 and 112 feed samples were collected between April and December 2015 in 13 case and 14 control farms, respectively. The 27 farms were randomly selected pig fattening farms located in different parts of Switzerland. The number of feed samples that contained Ent-Tetr as well as the number of Enterobacteriaceae resistant to tetracycline per sample was significantly higher in the case group than in the control group. The use of any of the 3 antimicrobial combinations turned out to be the main risk factor for the occurrence of Ent-Tetr in the liquid feed. Our results suggest that liquid feed containing antimicrobials is a reservoir of antimicrobial resistant bacteria in swine production.

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Increased production of biofilms by Escherichia coli in the presence of enrofloxacin

Cited by 31 publications

References 14 publications

Biofilm Formation on Biotic and Abiotic Surfaces in the Presence of Antimicrobials by Escherichia coli Isolates from Cases of Bovine Mastitis

Biofilm Formation on Biotic and Abiotic Surfaces in the Presence of Antimicrobials by Escherichia coli Isolates from Cases of Bovine Mastitis

Biofilm-Producing Ability of Bovine Extraintestinal Pathogenic Escherichia coli and Its Correlation with Attachment Factors

The effect of the administration of three different antimicrobial premix formulations via the liquid feeding system on the occurrence of Enterobacteriaceae resistant to tetracycline in the liquid feed for pigs

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