Increased release of bone sialoprotein into synovial fluid reflects tissue destruction in rheumatoid arthritis

Saxne, Tore; Zunino, L.; Heinegård, Dick

doi:10.1002/art.1780380113

Cited by 50 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, immunoassay techniques have been developed that measure immunoreactive BSP in serum [155,156]. All these assays are based on polyclonal antisera, and little is known about the spec®c nature of the respective epitopes.…”

Section: Bone Sialoprotein (Bsp)mentioning

confidence: 99%

Molecular Markers of Bone Turnover: Biochemical, Technical and Analytical Aspects

Seibel

2000

Osteoporosis International

151

114

View full text Add to dashboard Cite

Section: Bone Sialoprotein (Bsp)mentioning

confidence: 99%

Molecular Markers of Bone Turnover: Biochemical, Technical and Analytical Aspects

Seibel

2000

Osteoporosis International

151

114

View full text Add to dashboard Cite

“…Concentrations were determined by inhibition ELISA (17). Microtiter plates were coated with purified human BSP and an antiserum raised in a rabbit against human BSP was used as first antibody.…”

Section: Immunoassaysmentioning

confidence: 99%

“…BSP is particularly enriched in the cartilage-bone interphase (16). Synovial fluid levels of the protein are higher in RA patients with advanced joint destruction than in patients with well preserved joints ( 17 ). COMP is a cartilage specific protein with the highest concentrations being found in articular cartilage (18).…”

Section: Introductionmentioning

confidence: 99%

Cartilage and bone metabolism in rheumatoid arthritis. Differences between rapid and slow progression of disease identified by serum markers of cartilage metabolism.

Månsson

Carey²,

Alini³

et al. 1995

J. Clin. Invest.

222

161

View full text Add to dashboard Cite

Serum concentrations of specific cartilage and bone molecules reflecting tissue turnover were measured in two well-defined patient groups with early rheumatoid arthritis with distinctly different disease outcome to see if early differences in their levels are prognostic of the rate of joint destruction. Compared with a matched normal population, increased concentrations of cartilage oligomeric matrix protein (COMP) were found in all patients who developed rapid hip joint destruction. In contrast, levels of a putative marker of cartilage aggrecan synthesis, the chondroitin sulfate epitope 846, were increased only in patients with slow joint destruction. Levels of bone sialoprotein (BSP) were increased in both groups, as were levels of the C-propeptide of type II procoliagen (CPU), a marker of collagen II synthesis.The increased concentrations of the 846 epitope in patients with slow joint destruction suggest increased aggrecan synthesis. The low levels of the 846 epitope in patients with rapid joint destruction, concomitant with elevated levels of CPII, suggest a selective increase in collagen synthesis. The elevated BSP levels indicate an increased bone turnover in both groups. Thus elevated serum levels of COMP may indicate an unfavorable prognosis for rapid joint destruction, whereas elevated 846 epitope indicates a more favorable prognosis. (J. Clin. Invest. 1995. 95:1071-1077

show abstract

“…(25,(28)(29)(30)(31)(32)(33) Several metabolic bone diseases characterized by excessive bone resorption, including postmenopausal osteoporosis, Paget's disease, ankylosing spondylitis, and rheumatoid arthritis have also been associated with abnormally high BSP level in serum or synovial fluid. (34)(35)(36)(37) In contrast, treatments aimed at reducing excessive bone loss in postmenopausal women or patients with Paget's disease have been reported to decrease serum BSP level. (38,39) Although BSP expression in soft tissues and high serum BSP level are apparently associated with ectopic calcification and pathological bone remodeling, the effects of BSP overexpression in a transgenic mouse model remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Bone Sialoprotein Leads to an Uncoupling of Bone Formation and Bone Resorption in Mice

Valverde

Zhang

Fix

et al. 2008

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

ABSTRACT:The purpose of this study was to determine the effects of bone sialoprotein (BSP) overexpression in bone metabolism in vivo by using a homozygous transgenic mouse line that constitutively overexpresses mouse BSP cDNA driven by the cytomegalovirus (CMV) promoter. CMV-BSP transgenic (TG) mice and wildtype mice were weighed, and their length, BMD, and trabecular bone volume were measured. Serum levels of RANKL, osteocalcin, osteoprotegerin (OPG), TRACP5b, and PTH were determined. Bone histomorphometry, von Kossa staining, RT-PCR analysis, Western blot, MTS assay, in vitro mineralization assay, and TRACP staining were also performed to delineate phenotypes of this transgenic mouse line. Compared with wildtype mice, adult TG mice exhibit mild dwarfism, lower values of BMD, and lower trabecular bone volume. TG mice serum contained increased calcium levels and decreased PTH levels, whereas the levels of phosphorus and magnesium were within normal limits. TG mice serum also exhibited lower levels of osteoblast differentiation markers and higher levels of markers, indicating osteoclastic activity and bone resorption. H&E staining, TRACP staining, and bone histomorphometry showed that adult TG bones were thinner and the number of giant osteoclasts in TG mice was higher, whereas there were no significant alterations in osteoblast numbers between TG mice and WT mice. Furthermore, the vertical length of the hypertrophic zone in TG mice was slightly enlarged. Moreover, ex vivo experiments indicated that overexpression of BSP decreased osteoblast population and increased osteoclastic activity. Partly because of its effects in enhancing osteoclastic activity and decreasing osteoblast population, BSP overexpression leads to an uncoupling of bone formation and resorption, which in turn results in osteopenia and mild dwarfism in mice. These findings are expected to help the development of therapies to metabolic bone diseases characterized by high serum level of BSP.

show abstract

Increased release of bone sialoprotein into synovial fluid reflects tissue destruction in rheumatoid arthritis

Cited by 50 publications

References 31 publications

Molecular Markers of Bone Turnover: Biochemical, Technical and Analytical Aspects

Molecular Markers of Bone Turnover: Biochemical, Technical and Analytical Aspects

Cartilage and bone metabolism in rheumatoid arthritis. Differences between rapid and slow progression of disease identified by serum markers of cartilage metabolism.

Overexpression of Bone Sialoprotein Leads to an Uncoupling of Bone Formation and Bone Resorption in Mice

Contact Info

Product

Resources

About