Increased resistance of bacteria after adherence to polymethyl methacrylate

Arizono, Takeshi; Oga, Masayoshi; Sugioka, Yôichi

doi:10.1080/17453679209169731

Cited by 21 publications

(6 citation statements)

References 13 publications

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“…18,19 It has been shown that the first few hours before the formation of the complete biofilm and its coating with the slime layer are critical for the further antibiotic treatment of sessile bacteria. 20 We observed a similar phenomenon when amikacin in a high dose (over 16 times the MIC) was present in the incubation medium before the bacterial adhesion had started; then a reduction of over 2 log 10 units of bacteria attached was observed. Thus we believe that an early and sustained release of a drug(s) at a high concentration may be an optimal approach for controlling the microbial colonization of collagen biomaterials.…”

Section: Discussionsupporting

confidence: 57%

Adhesion ofPseudomonas aeruginosa to collagen biomaterials: Effect of amikacin and ciprofloxacin on the colonization and survival of the adherent organisms

Trafny

Kowalska

Grzybowski

1998

J. Biomed. Mater. Res.

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The adherence of P. aeruginosa to collagen membrane, sponge, and to a new anti-infective COLL dressing and the susceptibility of the organisms attached to the biomaterials to amikacin were investigated in vitro. After 17 h of attachment, the bacteria demonstrated an increased resistance to amikacin compared with their free-floating counterparts. Amikacin, even at a concentration exceeding 150 times the minimal bactericidal concentration (MBC) for the strain tested, did not eradicate the attached bacteria from the surface of collagen membrane. However, when the drug at a high concentration (over 16 times the minimal inhibitory concentration, MIC) was present in the incubation medium before it had been inoculated with P. aeruginosa, a reduction of 2 log10 units in the organisms adherent to the surface of collagen membrane was observed. We conclude that slow release of the antibiotic from the COLL dressing could control the bacterial colonization on the surface. In fact, the released amikacin at the final concentration of 32 times the MBC reduced the number of adherent bacteria by 6 log10 units. In contrast, ciprofloxacin at the same final bactericidal concentration completely eradicated the bacteria from the surface of COLL dressing. However, as ciprofloxacin is not recommended for use as a topical antimicrobial agent, a further search is needed to find an agent with a similar anticolonization activity.

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Section: Discussionsupporting

confidence: 57%

Adhesion ofPseudomonas aeruginosa to collagen biomaterials: Effect of amikacin and ciprofloxacin on the colonization and survival of the adherent organisms

Trafny

Kowalska

Grzybowski

1998

J. Biomed. Mater. Res.

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“…The pathogenic mechanisms of this refractoriness to treatment, however, have remained obscure. Contributory factors include bacterial virulence properties, such as slime production (4,10,23,31,56,60), as well as properties of specific biomaterials which increase microbial adherence or alter inflammatory changes (1,2,7,9,12,23,24,27,30,51,53,58,70,71,77,78) and impaired phagocytic host defenses even in the absence of microbial colonization (34-36, 57, 59, 64, 78, 79).…”

mentioning

confidence: 99%

Defensins Impair Phagocytic Killing by Neutrophils in Biomaterial-Related Infection

1999

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The implantation of foreign material carries a risk of infection which frequently is resistant to all treatment short of removing the implant. We have previously shown that these materials activate neutrophils by contact, leading to production of oxygen free radicals accompanied by release of granule products. Such activation further results in depletion of local host defenses, including the capacity of biomaterial-activated neutrophils to kill bacteria. Among the granule products released from neutrophils are small cationic antibacterial peptides (human neutrophil peptides [HNP]) known as defensins. Here we tested the hypothesis that defensins, released from activated neutrophils onto the surface of biomaterials, might play a role in the deactivation of subsequent neutrophil populations. Incubation of neutrophils with purified HNP resulted in a dose-related impairment of stimulus-induced oxygen radical production and of phagocytic killing. Furthermore, fresh neutrophils added to biomaterial-associated neutrophils exhibited impaired phagocytic killing. This impairment could be abrogated by antibody to HNP but not by an irrelevant antibody. Taken together, these observations support the idea that neutrophils activated at a material surface can create, by means of HNP release, an environment hostile to their microbicidal function and that of their infiltrating brethren. MATERIALS AND METHODS Cells and reagents. Blood was obtained from healthy human donors. Neutrophils were isolated by density gradient centrifugation according to the method of

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“…The bacterial growths with biofilms are able to resist several times of the lethal dose of the antibacterial agent (20, 23). Many approaches have been developed to make a biomaterial surface that has an antibiotic activity to reduce bacterial colonization and the formation of biofilm.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable Polymer Releasing Antibiotic Developed for Drainage Catheter of Cerebrospinal Fluid: In Vitro Results

et al. 2005

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The authors developed a biodegradable polymer that releases an antibiotic (nalidixic acid) slowly and continuously, for prevention of catheter-induced infection during drainage of cerebrospinal fluid. We investigated the in vitro antibiotic releasing characteristics and bacterial killing effects of the new polymer against E. coli. The novel fluoroquinolone polymer was prepared using diisopropylcarbodiimide, poly (e-caprolactone) diol, and nalidixic acid. FT-IR, mass spectrometry, and elemental analysis proved that the novel antibacterial polymer was prepared successfully without any side products. Negative MS showed that the released drug has a similar molecular weight (M.W.=232, 350) to pure drug (M.W.=232). In high pressure liquid chromatography, the released drug and drug-oligomer showed similar retention times (about 4.5-5 min) in comparison to pure drug (4.5 min). The released nalidixic acid and nalidixic acid derivatives have antibacterial characteristics against E. coli, Staphylococcus aureus, and Salmonella typhi, of more than 3 months duration. This study suggests the possibility of applying this new polymer to manufacture drainage catheters that resist catheter-induced infection, by delivering antibiotics for a longer period of more than 1 month.

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Increased resistance of bacteria after adherence to polymethyl methacrylate

Cited by 21 publications

References 13 publications

Adhesion ofPseudomonas aeruginosa to collagen biomaterials: Effect of amikacin and ciprofloxacin on the colonization and survival of the adherent organisms

Adhesion ofPseudomonas aeruginosa to collagen biomaterials: Effect of amikacin and ciprofloxacin on the colonization and survival of the adherent organisms

Defensins Impair Phagocytic Killing by Neutrophils in Biomaterial-Related Infection

Biodegradable Polymer Releasing Antibiotic Developed for Drainage Catheter of Cerebrospinal Fluid: In Vitro Results

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