Increased salinity improves the thermotolerance of mesophilic nitrification

Courtens, Emilie; Boon, Nico; Schryver, Peter De; Vlaeminck, Siegfried E.

doi:10.1007/s00253-014-5540-y

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…These advantages apply not only to warm wastewaters but also to wastewaters on sites with excess heat available. A few lab-scale studies have explored the potential of thermophilic nitrification for wastewater treatment, but achieved no more than 40-42.5°C (Shore et al, 2012;Courtens et al, 2014a). Thus far, this is the first study describing a thermophilic nitrifying bioreactor at 50°C.…”

Section: Discussionmentioning

confidence: 99%

“…Lopez-Vazquez et al (2014) recently reported nitrifying activity up to 50°C in mesophilic sludge from an industrial wastewater treatment plant after temperature shocking of mesophilic biomass (34°C) in short-term batch activity assays. However, the observations by Courtens et al (2014a) pointed out that prolonged exposure (48 h) of mesophilic biomass to a temperature shock can lead to a complete loss of nitrifying activity. To our knowledge, there is no description of nitrification at 50°C or above for an extended period of time.…”

Section: Introductionmentioning

confidence: 99%

“…As neither nitrite nor nitrate was ever measured in these bioreactors at 45-60°C, there is no evidence that nitrification took place in these thermophilic systems. Until now, only a few bioreactor studies focused on the long-term establishment of coupled thermophilic ammonia and nitrite oxidation, yet reaching no more than 42°C (Shore et al, 2012;Courtens et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

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A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

et al. 2016

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The increasing production of nitrogen-containing fertilizers is crucial to meet the global food demand, yet high losses of reactive nitrogen associated with the food production/consumption chain progressively deteriorate the natural environment. Currently, mesophilic nitrogen-removing microbes eliminate nitrogen from wastewaters. Although thermophilic nitrifiers have been separately enriched from natural environments, no bioreactors are described that couple these processes for the treatment of nitrogen in hot wastewaters. Samples from composting facilities were used as inoculum for the batch-wise enrichment of thermophilic nitrifiers (350 days). Subsequently, the enrichments were transferred to a bioreactor to obtain a stable, high-rate nitrifying process (560 days). The community contained up to 17% ammonia-oxidizing archaea (AOAs) closely related to 'Candidatus Nitrososphaera gargensis', and 25% nitrite-oxidizing bacteria (NOBs) related to Nitrospira calida. Incorporation of 13 C-derived bicarbonate into the respective characteristic membrane lipids during nitrification supported their activity as autotrophs. Specific activities up to 198 ± 10 and 894 ± 81 mg N g − 1 VSS per day for AOAs and NOBs were measured, where NOBs were 33% more sensitive to free ammonia. The NOBs were extremely sensitive to free nitrous acid, whereas the AOAs could only be inhibited by high nitrite concentrations, independent of the free nitrous acid concentration. The observed difference in product/substrate inhibition could facilitate the development of NOB inhibition strategies to achieve more cost-effective processes such as deammonification. This study describes the enrichment of autotrophic thermophilic nitrifiers from a nutrient-rich environment and the successful operation of a thermophilic nitrifying bioreactor for the first time, facilitating opportunities for thermophilic nitrogen removal biotechnology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

et al. 2016

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“…The pH of the residual water in the aquaponic systems presented an average value of 7.5 after passing through the tilapia tanks. After the biofilter, the pH was reduced to average values of 6.0 to 6.5, depending on the nitrification process, as described by Courtens et al (2014). During periodic samplings, the pH oscillated between 5.5 and 6.9, controlled and similar to those used by Van Rijn (2013).…”

Section: Resultsmentioning

confidence: 94%

Lettuce production in hydroponic and fish-farming aquaponic under different channel slopes and nutrient solutions in the NFT system

Mendonça

Silva

Mendonça

et al. 2023

Rev. bras. eng. agríc. ambient.

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The slope of cultivation channels and types of nutrient solutions in hydroponics and aquaponics influence nutrient absorption and plant production. This research aimed to evaluate lettuce production under different channel slopes and nutrient solutions in hydroponic and aquaponic systems using the nutrient film technique (NFT). A randomized block design was used, with three replicates, in a 3 × 5 split-plot scheme, with three nutrient solutions (conventional hydroponic solution and two wastewaters from the tilapia fish diets, with 15 and 18% of crude protein) and five slopes (2, 4, 6, 8 and 10%) of cultivation channels (PVC tubes). The following variables were evaluated: fresh and dry mass of shoot and roots and the chemical elements of the solutions. In the tilapia feed, the nutrient solution with 18% of protein (wastewater) provides greater production and accumulation of nutrients (N and P) in the lettuce shoot. The slope of 8% on cultivation channels provides greater production of iceberg lettuce, cultivar Lucy Brown. The different slopes and nutrient solutions studied did not influence the potassium (K) accumulation in the lettuce shoot.

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“…Traditional denitritation/denitritation with removal of NO 2 À and/or NO 3 À and heterotrophic production of nitrogen gas can also affect TIN mass balance (Daigger, As mentioned earlier in Section 3.1.1, this is possible when high solids concentration and coarse bubble diffusion create a favorable environment for the formation of anoxic pockets where production of nitrogen gas occurs (McNamara et al, 2022). Finally, the TIN mass balance (Figure S3) could also be impacted by biological uptake of nitrogen through bacterial growth and likely accounts for a small portion (<5%) (Courtens et al, 2014). In our study, we focused on the fate of nitrogen at constant saturated DO, while future work should look at TN and other nitrogen species, such as N 2 O.…”

Section: Temperature Ph and Nitrogen Speciation Could Lead To Differe...mentioning

confidence: 99%

Lab‐scale data and microbial community structure suggest shortcut nitrogen removal as the predominant nitrogen removal mechanism in post‐aerobic digestion (PAD)

Sabba¹,

Redmond²,

Ruff³

et al. 2022

Water Environment Research

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Implementing an aerobic digestion step after anaerobic digestion, referred to as “post aerobic digestion” (PAD), can remove ammonia without the need for an external carbon source and destroy volatile solids. While this process has been documented at the lab‐scale and full‐scale, the mechanism for N removal and the corresponding microbial community that carries out this process have not been established. This research gap is important to fill because the nitrogen removal pathway has implications on aeration requirements and carbon demand, that is, short‐cut N‐removal requires less oxygen and carbon than simultaneous nitrification–denitrification. The aims of this research were to (i) determine if nitrite (NO2−) or nitrate (NO3−) dominates following ammonia removal and (ii) characterize the microbial community from PAD reactors. Here, lab‐scale PAD reactors were seeded with biomass from two different full‐scale PAD reactors. The lab‐scale reactors were fed with biomass from full‐scale reactors and operated in batch mode to quantify nitrogen species concentrations (ammonia, NH4+, NO2−, and NO3−) over time. Experimental results revealed that NO2− production rates were several orders of magnitude greater than NO3− production rates. Indeed, nitrite accumulation rate (NAR) was greater than 90% at most temperatures, confirming that shortcut nitrogen removal was the dominant NH4+ removal mechanism in PAD. Microbial community analysis via 16S rRNA sequencing indicated that ammonia oxidizing bacteria (AOB) were much more abundant than nitrite oxidizing bacteria (NOB). Overall, this study suggests that aeration requirements for post‐aerobic digestion should be based on NO2− shunt and not complete simultaneous nitrification denitrification. Practitioner Points AOB are a key feature of PAD microbial communities NOB are present, but in much lower abundance than AOB High nitrite accumulation ratio suggests shortcut nitrite as the main mechanism for nitrogen removal Nitritation in PAD reactors is sustained at temperatures as high as 40°C No ammonia oxidation occurred at 50°C implying different mechanisms of nitrogen removal including ammonia stripping

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Increased salinity improves the thermotolerance of mesophilic nitrification

Cited by 6 publications

References 30 publications

A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

Lettuce production in hydroponic and fish-farming aquaponic under different channel slopes and nutrient solutions in the NFT system

Lab‐scale data and microbial community structure suggest shortcut nitrogen removal as the predominant nitrogen removal mechanism in post‐aerobic digestion (PAD)

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