Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines

Xu, Yue; Cheng, Qiaochu; Yang, Boyu; Yu, Shanshan; Xu, Fan; Lü, Lin; Liang, Xiaoling

doi:10.1167/iovs.15-16854

Cited by 30 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, blood-circulating leukocytes may engage with adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule (VCAM) and selectins, on the surface of endothelial cells, and the adherence of these cells to the endothelial wall may result in the occlusion of capillaries (leukostasis). Such vascular–immune cell interactions contribute to microvascular damage by releasing cytokines and superoxide via respiratory burst, which alters the integrity of the NVU [ 28 , 29 ]. In the advanced stages of diabetic retinopathy, in which immune privilege is compromised, circulating immune cells and serum proteins may infiltrate the retina and vitreous, thus participating in chronic inflammation and retinal vascular and neuronal damage [ 30 ].…”

Section: Is Microvascular Disease a Primary Pathogenic Event In The Dmentioning

confidence: 99%

Neurodegeneration in diabetic retinopathy: does it really matter?

2018

View full text Add to dashboard Cite

The concept of diabetic retinopathy as a microvascular disease has evolved, in that it is now considered a more complex diabetic complication in which neurodegeneration plays a significant role. In this article we provide a critical overview of the role of microvascular abnormalities and neurodegeneration in the pathogenesis of diabetic retinopathy. A special emphasis is placed on the pathophysiology of the neurovascular unit (NVU), including the contributions of microvascular and neural elements. The potential mechanisms linking retinal neurodegeneration and early microvascular impairment, and the effects of neuroprotective drugs are summarised. Additionally, we discuss how the assessment of retinal neurodegeneration could be an important index of cognitive status, thus helping to identify individuals at risk of dementia, which will impact on current procedures for diabetes management. We conclude that glial, neural and microvascular dysfunction are interdependent and essential for the development of diabetic retinopathy. Despite this intricate relationship, retinal neurodegeneration is a critical endpoint and neuroprotection, itself, can be considered a therapeutic target, independently of its potential impact on microvascular disease. In addition, interventional studies targeting pathogenic pathways that impact the NVU are needed. Findings from these studies will be crucial, not only for increasing our understanding of diabetic retinopathy, but also to help to implement a timely and efficient personalised medicine approach for treating this diabetic complication.Electronic supplementary materialThe online version of this article (10.1007/s00125-018-4692-1) contains a slideset of the figures for download, which is available to authorised users.

show abstract

Section: Is Microvascular Disease a Primary Pathogenic Event In The Dmentioning

confidence: 99%

Neurodegeneration in diabetic retinopathy: does it really matter?

2018

View full text Add to dashboard Cite

show abstract

“…The concentrations of VEGF-B and NLRP3 were below the limit of ELISA detection, thus the two cytokines were not included. The upregulated cytokines in PDR vitreous were also compared with publications (25)(26)(27)(28)(29) (Supplementary Table 1). Here, we identified 23 cytokines that significantly upregulated in PDR vitreous compared to control (Figure 6A).…”

Section: Profibrotic and Fibrogenic Phenotype Of Microglia Orchestrates The Progression Of Fvm Formationmentioning

confidence: 99%

Single-Cell Transcriptomics Reveals Novel Role of Microglia in Fibrovascular Membrane of Proliferative Diabetic Retinopathy

Mao

Chen

et al. 2022

Diabetes

View full text Add to dashboard Cite

Vitreous fibrovascular membranes (FVMs), the hallmark of proliferative diabetic retinopathy (PDR), cause retinal hemorrhage, detachment and eventually blindness. However, little is known about the pathophysiology of FVM. In this study, we employed single-cell RNA sequencing on surgically harvested PDR-FVMs and generated a comprehensive cell atlas of FVM. A total of 8 cellular compositions were identified, with microglia as the major cell population. We identified a GPNMB+ subpopulation of microglia, which presented both profibrotic and fibrogenic properties. Pseudotime analysis further revealed the profibrotic microglia was uniquely differentiated from retina-resident microglia and expanded in PDR setting. Ligand-receptor interactions between the profibrotic microglia and cytokines upregulated in PDR vitreous implicated the involvement of several pathways, including CCR5, IFNGR1 and CD44 signaling, in the microglial activation within PDR microenvironment. Collectively, our description of the novel microglia phenotypes in PDR-FVM may offer new insight into the cellular and molecular mechanism underlying the pathogenesis of DR, as well as potential signaling pathways amenable to disease-specific intervention.

show abstract

“…This study also showed that vitreous levels of sCD200 are higher in PDR patients with DME (266.9 ± 28.82 pg/mL) or traction retinal detachment (TRD) (256.9 ± 34.50 pg/mL) compared to PDR patients without DME (136.9 ± 15.13 pg/mL) or TRD (146.9 ± 15.97 pg/mL). sCD200 level increases also have significant statistical correlations with the increase of several angiogenic and inflammatory molecules such as VEGF, IL-6, IL-8 and IL-10 [115].…”

Section: Soluble Cd200mentioning

confidence: 89%

“…CD200 exists in a cell membrane-bound form and a soluble form. It exerts inhibitory effects on microglia/ macrophages via interaction with the CD200 receptor (CD200R) [115]. DR-related neuronal degeneration also reduces CD200 concentration and further induces microglial activation [6].…”

Section: Soluble Cd200mentioning

confidence: 99%

Proliferative Diabetic Retinopathy: An Overview of Vitreous Immune and Biomarkers

Victor¹,

Sitompul²

2018

Early Events in Diabetic Retinopathy and Intervention Strategies

View full text Add to dashboard Cite

This chapter discusses about the effect of vitreous immune system and biomarkers on the progression of proliferative diabetic retinopathy. Immune system and biomarkers have been believed to have an important role in the progression of diabetic retinopathy (DR) severity. Hyperglycemic will influence immune cells resulting in chronic inflammation on the retina. This condition progressively disrupts the blood-retinal barrier in retina causing those inflammatory molecules and immune cells to transfer from circulation. The transfer of these molecules plays an important part in the progression of proliferative diabetic retinopathy. In addition, biomarkers are indicators for some complex processes happened in our body, and are measured to determine diagnosis and prognosis of some treatment. There are several biomarkers that have been identified in DR patients including biomarkers of oxidative stress, hypoxia-inducible factors, angiogenic factors, pro-inflammatory cytokines, chemokines, cell adhesion molecules, and soluble CD200. The value of these biomarkers will tell us their possible role in the progression of DR. By improving the knowledge of molecular pathway in DR pathophysiology, the advancement of selective therapy approaches could be discovered and the management of DR could be more efficient.

show abstract

Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines

Abstract: Expression of sCD200 may contribute to retinal angiogenesis by interacting with VEGF-mediated inflammatory response and represents a potential therapeutic target for the patients with PDR.

Cited by 30 publications

References 35 publications

Neurodegeneration in diabetic retinopathy: does it really matter?

Neurodegeneration in diabetic retinopathy: does it really matter?

Single-Cell Transcriptomics Reveals Novel Role of Microglia in Fibrovascular Membrane of Proliferative Diabetic Retinopathy

Proliferative Diabetic Retinopathy: An Overview of Vitreous Immune and Biomarkers

Contact Info

Product

Resources

About