Increased sensitivity of RT-PCR for Potato virus Y detection using RNA isolated by a procedure with differential centrifugation

Zhang, Jianhua; Nie, Xianzhou; Boquel, Sébastien; Al-Daoud, Fadi; Pelletier, Yvan

doi:10.1016/j.jviromet.2015.07.008

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Specificity of primers and quality of template are two main factors that influence virus detection especially when virus concentration is low (Zhang et al 2015). Pre-analysis activities are critical and reproducible, therefore, the efficient protocol is a necessity (Martinelli et al 2015).…”

Section: Primers Design For Genetic Materials Of Potato Plants Virusesmentioning

confidence: 99%

“…Pre-analysis activities are critical and reproducible, therefore, the efficient protocol is a necessity (Martinelli et al 2015). Reports have been published showing the protocols of genetic materials extraction and primers designing (Nie and Singh 2015;Zhang et al 2015;Arruabarrena et al 2016). These reports have shown the use of differential centrifugation followed by RNeasy mini kit (DCR method) to increase the efficiency of RT-PCR technique.…”

Section: Primers Design For Genetic Materials Of Potato Plants Virusesmentioning

confidence: 99%

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Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

2019

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Potato is an economically important agro-industrial crop that is conventionally propagated, however; its potential transmission of viruses through seed tubers from generation to generation is a major limitation of potato yield production. In order to produce potato virus-free and sufficient amount of potato seed tubers, several approaches of in vitro methods for virus elimination have been developed. Meristem culture has been used alone or combined with techniques such as thermotherapy, electrotherapy, cryotherapy and chemotherapy as the best alternative method for treating potato infected by viruses. Recent literature has shown that to eliminate potato virus significantly depends upon the potato cultivar, antiviral agents, type of virus, the duration of heat treatment. Appropriate duration for efficiency elimination is still under investigation. Viral elimination rate can be detected through serological methods such as enzyme-linked immunosorbent assay (ELISA) and molecular biology technique such as real time reverse transcriptase polymerase chain reaction (real time RT-PCR) that are used for pre and post elimination virus detection to evaluate the efficiency and the accuracy of virus elimination method. The purpose of this review is to highlight virus elimination methods in potato and recommending the most effective tool for virus detection in order to ensure the production of potato plantlet free of viruses. Resumen La papa es un cultivo de importancia económica agro-industrial que se propaga de manera convencional, no obstante, la potencial transmisión de virus a través del tubérculo-semilla de generación en generación es una limitación mayor en la producción del rendimiento. Con el fin de producir papa libre de virus y suficiente cantidad de tubérculo-semilla de papa, se han desarrollado varias estrategias de métodos in vitro para la eliminación de los virus. El cultivo de meristemos se ha usado solo o combinado con técnicas tales como termoterapia, electroterapia, crioterapia y quimioterapia, como el mejor método alternativo para tratar papa infectada por virus. La literatura reciente ha demostrado que para eliminar virus significativamente se depende de la variedad de papa, agentes antivirales, tipo de virus, la duración del tratamiento térmico. Aun esta bajo investigación la duración apropiada para la eliminación eficiente. El nivel de la eliminación del virus puede detectarse por métodos serológicos, tales como el inmunoensayo con enzimas conjugadas (ELISA) y técnica de biología molecular, como la reverso-transcripción de reacción en cadena de la polimerasa en tiempo real (RT-PCR) que se usan para la detección del virus pre y post eliminación para evaluar la eficiencia y la precisión del método de eliminación del virus. El propósito de esta revisión es resaltar los métodos de eliminación de virus en papa y en la recomendación de la herramienta más efectiva para la detección de virus para asegurar la producción de plántulas de papa libres de virus.

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Section: Primers Design For Genetic Materials Of Potato Plants Virusesmentioning

confidence: 99%

Section: Primers Design For Genetic Materials Of Potato Plants Virusesmentioning

confidence: 99%

Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

2019

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Effects of dietary supplementation with icariin on growth performance, antioxidant capacity and non-specific immunity of Chinese mitten crab (Eriocheir sinensis)

Zheng

Chi

et al. 2019

Fish & Shellfish Immunology

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Production of Virus-Free Rose Plants using Meristem-Tip Culture and in vitro Thermotherapy

Aljuaid¹,

Attia²,

Ismail³

et al. 2022

Pakistan J. of Biological Sciences

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Increased sensitivity of RT-PCR for Potato virus Y detection using RNA isolated by a procedure with differential centrifugation

Cited by 3 publications

References 29 publications

Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

Effects of dietary supplementation with icariin on growth performance, antioxidant capacity and non-specific immunity of Chinese mitten crab (Eriocheir sinensis)

Production of Virus-Free Rose Plants using Meristem-Tip Culture and in vitro Thermotherapy

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