Increased Serum Oxidative Stress Markers in Women with Uterine Leiomyoma

Abstract

BackgroundUterine leiomyomas (fibroids) are the most common gynaecological benign tumors in premenopausal women. Evidences support the role of oxidative stress in the development of uterine leiomyoma. We have analysed oxidative stress markers (thiols, advanced oxidized protein products (AOPP), protein carbonyls and nitrates/nitrites) in preoperative sera from women with histologically proven uterine leiomyoma.Methodology/Principal FindingsWe conducted a laboratory study in a tertiary-care university hospital. Fifty-nine women with histologically proven uterine leiomyoma and ninety-two leiomyoma-free control women have been enrolled in this study. Complete surgical exploration of the abdominopelvic cavity was performed in each patient. Preoperative serum samples were obtained from all study participants to assay serum thiols, AOPP, protein carbonyls and nitrates/nitrites.Concentrations of serum protein carbonyl groups and AOPP were higher in leiomyoma patients than in the control group (p=0.005 and p<0.001, respectively). By contrast, serum thiol levels were lower in leiomyoma patients (p<0.001). We found positive correlations between serum AOPP concentrations and total fibroids weight (r=0.339; p=0.028), serum AOPP and serum protein carbonyls with duration of infertility (r=0.762; p=0.006 and r=0.683; p=0.021, respectively).Conclusions/SignificanceThis study, for the first time, reveals a significant increase of protein oxidative stress status and reduced antioxidant capacity in sera from women with uterine leiomyoma.

Level of Certain Oxidants and Antioxidants in Patients with Uterine Fibroids

“…Uterine fibroids are very common gynecological pathologies and have been tried to be associated with oxidative pathways (26). Individuals with uterine fibroids were found to have lower serum thiol levels and higher oxidative protein products compared to individuals in the control group (27). Similar results have been shown in different studies and it has been shown that both oxidative and antioxidative capacities are increased (28).…”

Plasma oxidative stress biomarkers and progesterone profiles in a dairy cow diagnosed with an ovarian follicular cyst

“…In humans, AOPP increase has been associated with several immuno-inflammatory markers and they have been proposed as an exquisite marker of OS correlating with monocyte activation (Witko-Sarsat et al 1998). The prolonged half-life of AOPP allows an indirect reflection of the intensity of the OS (Santulli et al 2013) and has been reported to be elevated in dairy cows with embryonic mortality (Celi et al 2011). The AOPP level was elevated at 9 h and this may have been associated with the development of the cyst in cow 1258; however, this cannot be concluded definitively from the results of this study.…”

Dysfunctional MnSOD leads to redox dysregulation and activation of prosurvival AKT signaling in uterine leiomyomas

“…Previous studies showed that multiple oxidative stress biomarkers, such as NOX4 ( 16 ), 8-OH-dG ( 17 ), and malonaldehyde ( 44 ), as well as serum oxidative stress markers ( 45 ), are increased in ULM. Moreover, unlike immortalized MM cells, activity of both catalase and extracellular SOD3 is significantly reduced in immortalized ULM cells ( 46 ).…”