Increased Sirtuin expression, senescence regulating miRNAs, mtDNA, and Bifidobateria correlate with wellbeing and skin appearance after Sirtuin- activating drink

Abstract

Background: Sirtuins attract high attention considering their properties to reverse molecular hallmarks of aging and age-related disorders. Many secondary plant ingredients (SPI) are known for their sirtuin-activating activities as well as epigenetic regulation of telomers, autophagy, senolysis, DNA repair but also improvement of gut microbiota. Furthermore, prebiotics enhanced butyrate was shown to interact with SIRT pathways. This study investigated effects of a drink containing a mix of different SPIs in combination with galactooligosaccharides (GOS) and their effect on SIRT activation, markers of aging relevant mechanisms and gut microbiota composition in correlation with subjective wellbeing and skin structure appearance. Methods: We analyzed gene expression, mtDNA amount, and microbial composition in response to a sirtuin-activating drink in humans compared to a control group consuming a placebo. Food frequency, beauty, and general health questionnaires were asked, and a set of mRNAs and miRNAs were assessed using qPCR analysis. The gut composition was analyzed using Illumnia sequencing.Results: SPI increased SIRT1, SIRT3 and modulated cell cycle relevant miR16 and senescence regulating miR34 expression. Additionally, mtDNA amount was higher in the group consuming the active supplement indicating an improved mitochondrial activity. The combined effect of SPI and GOS lead to an increase of Actinobacteria, especially Bifidobacterium, but also Veillonellaceae which was not observed in the control group. Significant correlations between SIRT3 expression and the gut microbiota Bifidobacterium and Veillonellaceae were observed. Additionally, statistical analysis of subjects self-reporting indicated beneficial effects regarding beauty and wellbeing.Conclusion: Our results show that the combination of sirtuins inducing SPI and prebiotic GOS influences molecular pathways counteracting aging, senescence, inflammation, and enhanced groups of gut microbiota which are known to improve the innate and adaptive immune system. Keywords: secondary plant ingredients, prebiotic, Sirtuins, subjective wellbeing, Bifidobacterium

Interim report from a 2-year double-blind rct testing fermented papaya preparation on immune enhancement, endothelial health and qol in elderly adults

“…This suggests that FPP ® , by a multifaceted, subcellular mechanism, as well as nonredox modulatory properties, played by its fermentationderived moieties, proving effective in beneficially regulating programmed cell death, which may have a variety of effectors [72,73]. The discipline of functional food intervention in aging and cancer is indeed an arena of very promising research studies [74][75][76][77][78][79] In conclusion, this data represents the first batch of a larger study providing more data focusing on immune…”

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies

“…For instance, probiotics such as Lactobacillus rhamnosus and Lactobacillus delbrueckii downregulate the expression of miR-181a and miR-155 involved in the inflammatory response of systemic lupus erythematosus ( Vahidi et al, 2018 ). In addition, the intake of probiotics such as bifidobacterium modulates cell cycle senescence by regulating miR-34 and increasing Sirtuins 1 resulting in wellbeing and reduced aging ( Haslberger et al, 2021 ). Nicotinamide mononucleotide supplementation enhances the antiaging properties of the aging aorta by upregulating miR-146a, and miR-203 and downregulating miR-99b and miR-127 ( Figure 3 ) ( Kiss et al, 2019 ).…”