Increased torulene accumulation in red yeast Sporidiobolus pararoseus NGR as stress response to high salt conditions

Li, Chunji; Zhang, Ning; Li, Bingxue; Xu, Qing; Song, Jia; Wei, Na; Wang, Wenjing; Zou, Hongtao

doi:10.1016/j.foodchem.2017.06.033

Cited by 46 publications

(41 citation statements)

References 34 publications

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“…Torulene exhibits properties of provitamin A and anti-prostate cancer activity, and is a safe food additive [16,17]. Despite the benefits of torulene, its production does not upgrade to industrial scale due to its low production rate and the shortage of knowledge regarding the regulation of its biosynthesis and the optimized culture condition, in comparison with another yeast carotenoid like astaxanthin and β-carotene [13].Although several studies have been conducted to optimize the production of lipids and carotenoids by R. glutinis [18,19], only a few studies were tried to co-produce both products together [20][21][22][23]. It has been known that nutrient-limitation, especially nitrogen and sulfur with high glucose concentration, can induce lipid accumulation from R. glutinis [24][25][26].…”

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Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

et al. 2019

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Torulene is a promising pink pigment, produced only by yeasts and fungi, and its production is still in a developing stage due to the low production rate. Accordingly, this study focuses on maximizing torulene production by Rhodotorula glutinis using shaken flask fermentation. The effect of different nitrogen sources, and C/N and C/S ratios on lipid and carotenoid production by R. glutinis was studied using 60 g/L glucose. The largest cells filled with golden fluorescence lipid bodies were observed using fluorescence microscopy when peptone was used as a nitrogen source. The highest total pigment (0.947 mg/L) and carotenoid relative productivity (Car-RP) (89.04 µg/g) were obtained at C/N 146 and C/S 120, and with ammonium sulfate as a nitrogen source, with 62% torulene domination using High Performance Liquid Chromatography (HPLC) for identification. Under a high C/N ratio, regardless of the C/S ratio, the carotenoid synthesis rate decreased after three days while the lipid synthesis rate kept increasing to the sixth day. Interestingly, after adding 0.7 mM Al2(SO4)3 to the optimized medium, the total pigment and Car-RP (2.2 mg/L and 212.9 µg/g) sharply increased, producing around 2.16 mg/L torulene (98%) with around 50% decrease in lipid yield. This is the first report on the role of Al2(SO4)3 for enhancing torulene production under lipogenesis condition, which could be used as a potential tool for torulene production.

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Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

et al. 2019

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“…The presence of torularhodin and torulene has been described in many yeast and fungi, such as Rhodotorula sp., Neurospora sp., and Sporobolomyces sp. [23,24]. In recent years, there have been increasing studies related to torularhodin and torulene biosynthesis and applications [25].…”

Section: Isolation Of R Toruloides Rm11 Rm14 and Rm18mentioning

confidence: 99%

“…In addition, torulene yield of RM18 was also significantly increased compared to the control strain. In microorganisms, the primary and essential role of these carotenoids is protection against the negative influence of reactive forms of oxygen, radiation, and unfavorable environmental stress [24,26]. Thus, carotenoids will be rapidly accumulated and in large amounts in RM18 due to its mutagenesis in the inhibitory and toxic lignocellulosic hydrolysate in first round screening.…”

Section: Isolation Of R Toruloides Rm11 Rm14 and Rm18mentioning

confidence: 99%

Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate

Shen

et al. 2020

Biotechnol Biofuels

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Background: In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341.Results: RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86-and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Conclusion:Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications.

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“…Biomass of this yeast constitutes sources of carotenoid, lipid, exopolysaccharide, and enzyme [7,8]. Colony color of S. pararoseus includes shades of pink and red due to the presence of lipid droplets full of carotenoid pigments, containing β-carotene, torulene and torularhodin [9][10][11].…”

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Whole genome sequencing and comparative genomic analysis of oleaginous red yeast Sporobolomyces pararoseus NGR identifies candidate genes for biotechnological potential and ballistospores-shooting

Zhao

et al. 2020

BMC Genomics

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Background: Sporobolomyces pararoseus is regarded as an oleaginous red yeast, which synthesizes numerous valuable compounds with wide industrial usages. This species hold biotechnological interests in biodiesel, food and cosmetics industries. Moreover, the ballistospores-shooting promotes the colonizing of S. pararoseus in most terrestrial and marine ecosystems. However, very little is known about the basic genomic features of S. pararoseus. To assess the biotechnological potential and ballistospores-shooting mechanism of S. pararoseus on genome-scale, the whole genome sequencing was performed by next-generation sequencing technology. Results: Here, we used Illumina Hiseq platform to firstly assemble S. pararoseus genome into 20.9 Mb containing 54 scaffolds and 5963 predicted genes with a N50 length of 2,038,020 bp and GC content of 47.59%. Genome completeness (BUSCO alignment: 95.4%) and RNA-seq analysis (expressed genes: 98.68%) indicated the high-quality features of the current genome. Through the annotation information of the genome, we screened many key genes involved in carotenoids, lipids, carbohydrate metabolism and signal transduction pathways. A phylogenetic assessment suggested that the evolutionary trajectory of the order Sporidiobolales species was evolved from genus Sporobolomyces to Rhodotorula through the mediator Rhodosporidiobolus. Compared to the lacking ballistospores Rhodotorula toruloides and Saccharomyces cerevisiae, we found genes enriched for spore germination and sugar metabolism. These genes might be responsible for the ballistospores-shooting in S. pararoseus NGR. Conclusion: These results greatly advance our understanding of S. pararoseus NGR in biotechnological potential and ballistospores-shooting, which help further research of genetic manipulation, metabolic engineering as well as its evolutionary direction.

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Increased torulene accumulation in red yeast Sporidiobolus pararoseus NGR as stress response to high salt conditions

Cited by 46 publications

References 34 publications

Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate

Whole genome sequencing and comparative genomic analysis of oleaginous red yeast Sporobolomyces pararoseus NGR identifies candidate genes for biotechnological potential and ballistospores-shooting

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