Increased Vascular Endothelial Growth Factor Levels in the Vitreous of Eyes With Proliferative Diabetic Retinopathy

Adamis, P; Miller, J W; Bernal, Mireia; Amico, Donatella; Folkman, J; Yeo, Tet-Kin; Yeo, Kiang-Teck

doi:10.1097/00006982-199515020-00027

Cited by 382 publications

(462 citation statements)

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“…Our results show, for the first time, that Flt-1 domain 2 does not require presence of other VEGFR domains for high-affinity VEGF binding. Several other constructs where 9Gly was replaced with other short linkers like 15-mer (Gly 4 Ser) 3 , polyglycine pentamer (5Gly) or other random linkers showed VEGF binding comparable to sFLT01.…”

Section: Discussionmentioning

confidence: 99%

“…show a prevalence of the dimeric forms for both proteins under nonreducing conditions ruling out the possibility that the lack of efficacy of D2-CH3 is due to its inability to dimerize. For the next construct we used another type of linker, the 15-mer (Gly 4 Ser) 3 . 23 D2-(Gly 4 Ser) 3 -Fc protein was generated and it contained Flt-1 domain 2, (Gly 4 Ser) 3 linker and the IgG1 Fc.…”

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

“…For the next construct we used another type of linker, the 15-mer (Gly 4 Ser) 3 . 23 D2-(Gly 4 Ser) 3 -Fc protein was generated and it contained Flt-1 domain 2, (Gly 4 Ser) 3 linker and the IgG1 Fc. The molecule D2-(Gly 4 Ser) 3 -Fc was further characterized in HUVECs proliferation assay.…”

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

“…23 D2-(Gly 4 Ser) 3 -Fc protein was generated and it contained Flt-1 domain 2, (Gly 4 Ser) 3 linker and the IgG1 Fc. The molecule D2-(Gly 4 Ser) 3 -Fc was further characterized in HUVECs proliferation assay. Biological activity of D2-(Gly 4 Ser) 3 -Fc as measured by inhibition of HUVEC proliferation was similar to that of sFLT01 (data not shown).…”

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

“…1 Formation of new blood vessels caused by the overproduction of growth factors such as VEGF is a key component of diseases such as wet age-related macular degeneration (AMD), proliferative diabetic retinopathy (PDR) and tumor growth. [2][3][4] Blocking of VEGF with antibodies, soluble VEGF receptors (sVEGFR) or inhibition of VEGF receptor (VEGFR) tyrosine kinase activity are useful strategies that have shown promising preclinical and clinical results. [4][5][6] Ranibizumab, an anti-VEGF drug given intravitreally to wet-AMD patients results in substantially improved visual acuity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Pecháň¹,

Rubin²,

Lukason³

et al. 2008

Gene Ther

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Section: Discussionmentioning

confidence: 99%

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

Section: Igg1 Ch3 Domain and Peptide Linker Are Important In Vegf Binmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Pecháň¹,

Rubin²,

Lukason³

et al. 2008

Gene Ther

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Transforming Growth Factor β2 in the Vitreous in Proliferative Diabetic Retinopathy

Hirase¹

1998

Arch Ophthalmol

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To evaluate the hypothesis that transforming growth factor ␤ 2 (TGF-␤ 2) is involved in the cause of proliferative diabetic retinopathy (PDR). Methods: We assayed TGF-␤ 2 levels in the vitreous of patients with PDR and other vitreoretinal disorders. Fortynine vitreous specimens were obtained from eyes of patients with PDR undergoing vitrectomy, and 19 vitreous specimens from nondiabetic subjects served as controls. We assessed TGF-␤ 2 levels using an enzymelinked immunosorbent assay. Both mature and total TGF-␤ 2 levels were quantified. Results: The mean (± SD) total levels of TGF-␤ 2 were 2634 (± 1652) pg/mL in the patients with PDR and 1305 (± 972) pg/mL in controls. The mean (± SD) levels of mature TGF-␤ 2 were 244 (± 316) pg/mL in patients with PDR and 79 (± 81) pg/mL in controls. Total and mature TGF-␤ 2 levels were significantly greater in patients with PDR (total TGF-␤ 2 , P Ͻ.001; mature TGF-␤ 2 , P Ͻ.01). Mature TGF-␤ 2 levels were higher in the vitreous of patients who had severe fibrous proliferation. Conclusion: The results indicate increased levels of both total and mature TGF-␤ 2 in the vitreous of patients with PDR, suggesting that TGF-␤ 2 plays an important role in the pathogenesis of PDR.

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Angiography of Fluoresceinated Anti–Vascular Endothelial Growth Factor Antibody and Dextrans in Experimental Choroidal Neovascularization

Tolentino

Husain²,

Theodosiadis³

et al. 2000

Arch Ophthalmol

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To determine if anti-vascular endothelial growth factor antibody and a range of dextrans with varying diffusion radii and molecular weights are permeable through experimental choroidal neovascularization (CNV). Methods: Choroidal neovascularization was induced in 10 cynomolgus monkey retinas by means of argon laser injury. Digital fundus fluorescein angiograms were performed with fluorescein sodium, fluoresceinated IgG antibodies (anti-vascular endothelial growth factor and a control antibody), and fluoresceinated dextrans with molecular weights of 4, 20, 40, 70 and 150 kd. The 40-and 70-kd dextrans straddle the effective diffusion radius of IgG. For each reagent, early and late angiograms were performed in a standardized fashion, with follow-up images obtained to monitor residual fluorescence. Results: Perfusion of retinal vessels and choroidal vasculature was seen with all reagents. Fluorescein and 4and 20-kd dextran leaked rapidly from the CNV within the first minute. Angiography with the use of 40-kd dextran and fluoresceinated antibody, either anti-vascular

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Increased Vascular Endothelial Growth Factor Levels in the Vitreous of Eyes With Proliferative Diabetic Retinopathy

Cited by 382 publications

References 0 publications

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Transforming Growth Factor β2 in the Vitreous in Proliferative Diabetic Retinopathy

Angiography of Fluoresceinated Anti–Vascular Endothelial Growth Factor Antibody and Dextrans in Experimental Choroidal Neovascularization

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