Increasing Cellulose Production from Rhizobium leguminosarum bv. viciae

Abstract

Bacterial cellulose (BC) is a type of biopolymer produced as primary metabolism product by many genera of bacteria such as Acetobacter, Rhizobium, Agrobacterium, Psuedomonas and Sarcina. This article aimed to study the effect of some factors on cellulose production from Rhizobium leguminosarum bv. viciae which isolated from root nodules of Vicia faba plant collected from Abu-Ghraib in Baghdad. Some environmental and nutritional factors were examined including : carbon and nitrogen sources, pH, temperature and incubation period. Results showed that maximum production of cellulose was gained by Hestrin-Schram medium (HS medium) containing 2% fructose as carbon source, 1% yeast extract as nitrogen source, as well as optimum pH, temperature, and incubation period were (5, 30°C for 7 days) respectively .Cellulose dry weight was analyzed by Fourier transform infrared spectroscopy (FTIR) technique and the results showed the presence of the functional groups of bacterial cellulose.

Use of Industrial Wastes as Sustainable Nutrient Sources for Bacterial Cellulose (BC) Production: Mechanism, Advances, and Future Perspectives

“…Extensive research has been conducted on the extraction of cellulose fibre from various plant fibre, i.e., sugar palm fibre [ 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 ], water hyacinth [ 139 ], ginger fibre [ 140 , 141 ], kenaf [ 142 ], sugarcane [ 143 , 144 ], lemongrass [ 145 ], cassava, corn, oat, palm oil fibre, and others [ 146 , 147 , 148 ]. Next is cellulose biosynthesis by using different types of microorganisms; (i) bacteria (gram-negative: Alcaligenes [ 149 ], Salmonella , Enterobacter , Pseudomonas [ 150 ], Gluconacetobacter xylinus [ 151 ], Agrobacterium [ 152 ], Komagataeibacter Medellinensis [ 153 ], Aerobacter , Achromobacter insuavis [ 154 ], Rhizobium leguminosarum [ 155 ], Acetobacter spp. [ 156 ], Acetobacter xylinum [ 157 ], Zoogloea [ 97 ], and gram-positive: Sarcina ventriculi [ 158 ], Leifsonia sp [ 159 ], Rhizosphere bacterium , Bacillus subtilis [ 70 , 160 ]); (ii) fungi ( Aspergillus ornatus [ 161 ], Penicillium sp.…”