Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

Lavrinovičs, Aigars; Murby, Fredrika; Ziverte, Elina; Mežule, Linda; Juhna, Tālis

doi:10.3390/microorganisms9081598

Cited by 21 publications

(9 citation statements)

References 43 publications

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“…Both measurements were carried out using a spectrophotometer (V-5600, METASH, China) and commercial reagent sets. The percentage nutrient removal and nutrient uptake rate per cell were calculated from the changes of the nutrients over time and cell concentrations following Aigars et al 59 .…”

Section: Methodsmentioning

confidence: 99%

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

You

Sun

et al. 2022

Nat Commun

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Trypsin is best known as a digestive enzyme in animals, but remains unexplored in phytoplankton, the major primary producers in the ocean. Here we report the prevalence of trypsin genes in global ocean phytoplankton and significant influences of environmental nitrogen (N) and phosphorus (P) on their expression. Using CRISPR/Cas9 mediated-knockout and overexpression analyses, we further reveal that a trypsin in Phaeodactylum tricornutum (PtTryp2) functions to repress N acquisition, but its expression decreases under N-deficiency to promote N acquisition. On the contrary, PtTryp2 promotes phosphate uptake per se, and its expression increases under P-deficiency to further reinforce P acquisition. Furthermore, PtTryp2 knockout led to amplitude magnification of the nitrate and phosphate uptake ‘seesaw’, whereas PtTryp2 overexpression dampened it, linking PtTryp2 to stabilizing N:P stoichiometry. Our data demonstrate that PtTryp2 is a coordinate regulator of N:P stoichiometric homeostasis. The study opens a window for deciphering how phytoplankton adapt to nutrient-variable marine environments.

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Section: Methodsmentioning

confidence: 99%

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

You

Sun

et al. 2022

Nat Commun

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“…Biomass productivity ( g L -1 d -1 ) was determined with the equation Bp= (Bc f - Bc i /t , where Bc i , and Bc f are the biomass concentration initial and final values for the cultivation time (t=7 d), respectively. The nutrient uptake and removal rates for each line were calculated according to ( 53 ). The maximum nutrient removal rates R max (mg N L -1 d -1 ) were obtained by calculating the daily removal of a specific nutrient (CN d - CN d-1 ) where CN d is the nutrient concentration at a specific day and CN d-1 is the nutrient concentration on the day before, and finally selecting the highest removal rate observed on a specific day during the experiment.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Slocombe

Zúñiga-Burgos

LiLi

et al. 2022

Preprint

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Remediation using micro-algae offers an attractive solution to environmental phosphate (Pi) pollution. However, for maximum efficiency, pre-conditioning of algae to induce luxury phosphorus (P) uptake is needed. Here we show that natural pre-conditioning can be mimicked through over-expression of a single gene, the global regulator PSR1 (Myb transcription factor: Phosphate Starvation Response 1), raising P levels to 8% dry cell weight from 2% in control. Complete removal of Pi occurred in log phase, unlike the control. This was associated with increases in PolyP granule size and uptake of Mg2+, the principal counterion. Hyper-accumulation of P depended on a feed-forward mechanism, where a small set of Class I genes were activated despite abundant external Pi levels. This drove a reduction in external Pi levels, permitting more genes to be expressed (Class II), leading to more Pi -uptake. These discoveries enable a bio-circular approach of recycling nutrients from wastewater back to agriculture.

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“…Regulation of almost all P-related enzymes by intracellular phosphate content has been studied for various organisms [ 17 , 18 , 19 , 20 , 21 ]. The most common way of this regulation involves SPX domains, which are present in several enzymes that promote the transport of phosphate across the outer and inner membranes of the cell, as well as in transcriptional regulators [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Simulating the Interplay between the Uptake of Inorganic Phosphate and the Cell Phosphate Metabolism under Phosphorus Feast and Famine Conditions in Chlorella vulgaris

Plyusnina

Khruschev

Fursova

et al. 2021

Cells

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Using a mathematical simulation approach, we studied the dynamics of the green microalga Chlorella vulgaris phosphate metabolism response to shortage and subsequent replenishing of inorganic phosphate in the medium. A three-pool interaction model was used to describe the phosphate uptake from the medium, its incorporation into the cell organic compounds, its storage in the form of polyphosphates, and culture growth. The model comprises a system of ordinary differential equations. The distribution of phosphorous between cell pools was examined for three different stages of the experiment: growth in phosphate-rich medium, incubation in phosphate-free medium, and phosphate addition to the phosphorus-starving culture. Mathematical modeling offers two possible scenarios for the appearance of the peak of polyphosphates (PolyP). The first scenario explains the accumulation of PolyP by activation of the processes of its synthesis, and the decline in PolyP is due to its redistribution between dividing cells during growth. The second scenario includes a hysteretic mechanism for the regulation of PolyP hydrolysis, depending on the intracellular content of inorganic phosphate. The new model of the dynamics of P pools in the cell allows one to better understand the phenomena taking place during P starvation and re-feeding of the P-starved microalgal cultures with inorganic phosphate such as transient PolyP accumulation. Biotechnological implications of the observed dynamics of the polyphosphate pool of the microalgal cell are considered. An approach enhancing the microalgae-based wastewater treatment method based on these scenarios is proposed.

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Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

Cited by 21 publications

References 43 publications

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Simulating the Interplay between the Uptake of Inorganic Phosphate and the Cell Phosphate Metabolism under Phosphorus Feast and Famine Conditions in Chlorella vulgaris

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