Increasing prevalence of antibiotic resistance genes in manured agricultural soils in northern China

Wu, Nan; Zhang, Weiyu; Xie, Shouqi; Zeng, Ming; Liu, Haixue; Yang, Jinghui; Liu, Xinyuan; Yang, Fan

doi:10.1007/s11783-019-1180-x

Cited by 64 publications

(10 citation statements)

References 45 publications

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“…The drug has proven clinical safety, acceptable tolerability, and it is available in intravenous and oral formulations for most members of the class (Grossman 2016). Extensive use of tetracyclines in veterinary medicine and agricultural applications, including crop protection and intensive animal farming, has contributed to the widespread dissemination of tetracycline resistance (Surette and Wright 2017;Wu et al 2020). In this study, resistance genes encoding ribosomal protection proteins, tetracycline-inactivating enzymes, and tetracycline efflux were detected.…”

Section: Discussionmentioning

confidence: 82%

Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil

Salam¹

2020

3 Biotech

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The antibiotic and heavy metal resistome of a chronically polluted soil (3S) obtained from an automobile workshop in Ilorin, Kwara State, Nigeria was deciphered via functional annotation of putative ORFs (open reading frames). Functional annotation of antibiotic and heavy metal resistance genes in 3S metagenome was conducted using the Comprehensive Antibiotic Resistance Database (CARD), Antibiotic Resistance Gene-annotation (ARG-ANNOT) and Antibacterial Biocide and Metal Resistance Gene Database (BacMet). Annotation revealed detection of resistance genes for 15 antibiotic classes with the preponderance of beta lactamases, mobilized colistin resistance determinant (mcr), glycopepetide and tetracycline resistance genes, the OqxBgb and OqxA RND-type multidrug efflux pumps, among others. The dominance of resistance genes for antibiotics effective against members of the Enterobacteriaceae indicate possible contamination with faecal materials. Annotation of heavy metal resistance genes revealed diverse resistance genes responsible for the uptake, transport, detoxification, efflux and regulation of copper, zinc, cadmium, nickel, chromium, cobalt, mercury, arsenic, iron, molybdenum and several others. Majority of the antibiotic and heavy metal resistance genes detected in this study are borne on mobile genetic elements, which facilitate their spread and dissemination in the polluted soil. The presence of the heavy metal resistance genes is strongly believed to play a major role in the proliferation of antibiotic resistance genes. This study has established that soil is a huge repertoire of antibiotic and heavy metal resistome and due to the intricate link between human, animals and the soil environment, it may be a major contributor to the proliferation of multidrug-resistant clinical pathogens.

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Section: Discussionmentioning

confidence: 82%

Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil

Salam¹

2020

3 Biotech

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“…The plant rhizosphere is indeed a well characterized, substrate-rich, hot spot for bacterial activity and abundance (Zhu et al, 2018), where naturally competent cells can find higher concentrations of free DNA and could, moreover, reach the growth phase in which transformation occurs with high frequency (Sørensen and Jensen, 1998;Mølbak et al, 2003;Ling et al, 2016;Zhu et al, 2018). Relevant concentrations of ARGs can reach the plant rhizosphere, e.g., through soil amended with manure, sewage sludge and treated wastewater (Chen et al, 2017;Riva et al, 2020;Wu et al, 2020). We selected lettuce as a model plant for the root system colonization experiments, as representative of raw-consumed vegetables of high economic importance in the ready-to-eat food industry.…”

Section: Discussionmentioning

confidence: 99%

An Environmental Escherichia coli Strain Is Naturally Competent to Acquire Exogenous DNA

Riva

Eckert

et al. 2020

Front. Microbiol.

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The diffusion of antibiotic resistance determinants in different environments, e.g., soil and water, has become a public concern for global health and food safety and many efforts are currently devoted to clarify this complex ecological and evolutionary issue. Horizontal gene transfer (HGT) has an important role in the spread of antibiotic resistance genes (ARGs). However, among the different HGT mechanisms, the capacity of environmental bacteria to acquire naked exogenous DNA by natural competence is still poorly investigated. This study aimed to characterize the ability of the environmental Escherichia coli strain ED1, isolated from the crustacean Daphnia sp., to acquire exogenous DNA by natural competence. Transformation experiments were carried out varying different parameters, i.e., cell growth phase, amount of exogenous DNA and exposition to artificial lake water (ALW) and treated wastewater to mimic environmentallike conditions that may be encountered in the agri-food system. Results were compared with those showed by the laboratory E. coli strain DH5α. Our experimental data, supported by genomic sequencing, showed that, when exposed to pure water, ED1 strain was able to acquire exogenous DNA with frequencies (10 −8-10 −9) statistically higher than the ones observed for DH5α strain (10 −10). Interestingly, higher values were retrieved for ED1 than DH5α strains exposed to ALW (10 −7 vs. 10 −9 , respectively) or treated wastewater (10 −8 vs. 10 −10 , respectively). We tested, therefore, ED1 strain ability to colonize the rhizosphere of lettuce, a model plant representative of rawconsumed vegetables of high economic importance in the ready-to-eat food industry. Results showed that ED1 strain was able to efficiently colonize lettuce rhizosphere, revealing a stable colonization for 14 days-long period. In conclusion, ED1 strain ability to acquire exogenous DNA in environmental-like conditions by natural competence, combined with its ability to efficiently and stably colonize plant rhizosphere, poses the attention to food and human safety showing a possible route of diffusion of antibiotic resistance in the agri-food system, sustaining the "One Health" warnings related to the antibiotic spread.

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“…ARGs can proliferate in diverse matrices including microplastics (Lu et al, 2019a(Lu et al, , 2020a(Lu et al, , 2020b. ARGs generally propagate in the environment through vertical gene transfer by transmitting genetic information from mother to daughter cells and horizonal gene transfer (HGT) under mediation by mobile genetic elements (MGEs) such as transposons, plasmids, bacteriophages, and intergrons (Pazda et al, 2019;Wu et al, 2020). ARGs can be induced under the natural conditions while they also proliferate under the coselective pressure from antibiotics, heavy metals, or other agents (Li et al, 2020).…”

Section: G R a P H I C Abstract Abstractmentioning

confidence: 99%

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

Lü

Wang

2021

Front. Environ. Sci. Eng.

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Increasing prevalence of antibiotic resistance genes in manured agricultural soils in northern China

Cited by 64 publications

References 45 publications

Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil

Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil

An Environmental Escherichia coli Strain Is Naturally Competent to Acquire Exogenous DNA

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

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