Increasing procoagulant activity of circulating microparticles in patients living with HIV

Snopková, Svatava; Matýšková, Miloslava; Havlíčková, Kateřina; Jarkovský, Jiří; Svoboda, Michal; Zavřelová, Jiřina; Svačinka, Radek; Penka, Miroslav; Husa, Petr

doi:10.1016/j.medmal.2019.09.013

Cited by 7 publications

(10 citation statements)

References 38 publications

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“…In our previous study, MPs levels were analyzed in HIV-positive patients using the same method of detection 6 . Based on our results, MPs levels of HIV-positive patients both treated (8.9 nM) and untreated (9.0 nM) were higher than in the control group (3.2 nM) but lower than the newly studied HCV positive group.…”

Section: Discussionmentioning

confidence: 99%

“…Between 70 to 90% of MPs detected in the blood are derived from platelets, but other cells such as endothelial, leukocytes, erythrocytes, and even malignant cells could also shed MPs (ref. 5,6 ). They are released during cell activation by both chemical (eg, cytokines, thrombin, cytotoxic chemotherapy, hypercholesterolemia, and tobacco smoke exposure) and physical (eg, shear stress, hypoxia) stimulation 6 .…”

Section: Microparticlesmentioning

confidence: 99%

“…5,6 ). They are released during cell activation by both chemical (eg, cytokines, thrombin, cytotoxic chemotherapy, hypercholesterolemia, and tobacco smoke exposure) and physical (eg, shear stress, hypoxia) stimulation 6 . They also shed during apoptosis 7 and senescence of cells 8 .…”

Section: Microparticlesmentioning

confidence: 99%

“…MPs may transfer complete cell surface receptor signaling pathways into the recipient cell that is specific for the MPs releasing cell, exchange genetic information, or transfer antigen via MHC class II molecules 11 . They act as a communication tool for extracellular communication and transport between cells 6 . MPs participate in a variety of physiological functions, mainly including the promotion of blood clotting, endothelial repair, angiogenesis, and inflammatory responses, although their role in body physiology is not yet fully understood 12,13 .…”

Section: Microparticlesmentioning

confidence: 99%

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Circulating microparticles in patients with chronic hepatitis C and changes during direct-acting antiviral therapy

Husa

Snopková

Zavřelová

et al. 2021

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. Microparticles (MPs) are heterogeneous vesicles derived from membranes of different cells. Between 70 to 90% of MPs detected in blood originate from platelets. The release of MPs is associated with proinflammatory and procoagulant states. Elevated levels of MPs have been found in different diseases. We investigated MPs levels in patients with chronic hepatitis C (CHC) and changes in level during treatment using direct-acting antivirotics (DAA). Patients and Methods. Thirty-six patients with CHC and forty healthy volunteers were included in the study. Concentrations of MPs were determined indirectly by measuring their procoagulant activity in plasma at baseline, end of therapy (EOT), and 12 weeks after EOT when the sustained virological response was assessed (SVR12). Results. All patients achieved SVR12, which was associated with rapid improvement of markers of liver damage and function as well as liver stiffness (P=0.002). MPs levels were significantly higher in CHC patients than in healthy volunteers (P<0.001). No statistically significant decrease was found observed between baseline and SVR12 (P=0,330). Analysis of subpopulations with minimal fibrosis F0-1 (P=0.647), advanced fibrosis F2-4 (P=0.370), women(P=0.847), men (P=0.164) and genotype 1 (P=0.077) showed no significant changes during the follow-up period. Conclusions. MPs levels are higher in CHC patients and remain elevated shortly after achieving SVR. Higher concentrations of MPs in plasma are probably caused by a chronic uncontrolled exaggerated inflammatory response caused by CHC. Longer observation would probably confirm the significance of MPs levels decrease because normalization of liver function, inflammation, and structure after SVR requires more than 12 weeks.

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Section: Discussionmentioning

confidence: 99%

Section: Microparticlesmentioning

confidence: 99%

Section: Microparticlesmentioning

confidence: 99%

Section: Microparticlesmentioning

confidence: 99%

See 2 more Smart Citations

Circulating microparticles in patients with chronic hepatitis C and changes during direct-acting antiviral therapy

Husa

Snopková

Zavřelová

et al. 2021

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…A similar case control study of 15 PLWH established on ART in the USA, showed significantly elevated levels of all types of microparticles compared to 15 participants without HIV infection(23), where microparticles from PLWH directly impaired endothelial cell function. A larger study of PLWH from the Czech Republic also found an increase in microparticles compared to participants without HIV, but found no difference amongst those on ART compared to those who were at their first presentation (26). However, none of these studies assessed the relationship between CMPs and arterial stiffness.…”

Section: Discussionmentioning

confidence: 99%

Circulating Microparticles are increased amongst people presenting with HIV and advanced immune suppression in Malawi and correlate closely with arterial stiffness

Kelly¹,

Gurung

Tinago³

et al. 2020

Preprint

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Objectives We aim to investigate whether circulating microparticle (CMPs) subsets were raised amongst people living with HIV in Malawi, and whether they associated with arterial stiffness. Methods ART-naive adults with a new HIV diagnosis and CD4 <100 cells/uL had microparticle characterisation and carotid femoral Pulse Wave Velocity (cfPWV) at 2 weeks post ART initiation. HIV uninfected controls were matched on age, systolic BP and diastolic BP in a 1:1 ratio. Circulating microparticles were identified from platelet poor plasma and stained for endothelial, leucocyte, monocyte and platelet markers. Results The median (IQ) total CMP count was 12 fold higher in participants with HIV compared to those without (p<0.0001) and was associated with arterial stiffness (spearman rho 0.42, p<0.001). In adjusted analysis, every log increase in circulating particles showed a 20% increase in cfPWV (95% CI 4 to 40%, p=0.02). In terms of subsets, endothelial and platelet derived microparticles were most strongly associated with HIV. Endothelial derived Eselectin+ CMPs were 22.5 fold higher and platelet derived CD42a+ CMPs were 12 fold higher (both p<0.0001). Endothelial and platelet derived CMPs also correlated most closely with arterial stiffness [spearman rho: Eselectin+ 0.57 and CD42a 0.56, both p<0.0001). Conclusions Circulating microparticles associate strongly with HIV and arterial stiffness amongst adults in Malawi. Endothelial damage and platelet activation are of particular importance and future translational studies should consider prioritising this pathway.

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Circulating extracellular vesicles as new inflammation marker in HIV infection

Falasca

Lanuti

Ucciferri

et al. 2020

Aids

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Background: Extracellular vesicles, released by cell pullulation, are surrounded by a phospholipid bilayer and carry proteins as well and genetic material. It has been shown that extracellular vesicles mediate intercellular communication in several conditions, such as inflammation, immunodeficiency, tumor growth, and viral infections. Here, we analyzed circulating levels of extracellular vesicles in order to clarify their role in chronic inflammation mechanisms characterizing HIV patients. Methods: We analyzed and subtyped circulating levels of extracellular vesicles, through a recently developed flow cytometry method. In detail, endothelial-derived extracellular vesicles (CD31+/CD41a−/CD45−, EMVs), extracellular vesicles stemming from leukocytes (CD45+, LMVs) and platelets (CD41a+/CD31+) were identified and enumerated. Moreover, we analyzed the extracellular vesicle protein cargo with proteomic analysis. Results: Circulating levels of total extracellular vesicles, EMVs and LMVs were significantly lower in the HIV+ patients than in healthy subjects, whereas platelet-derived extracellular vesicles resulted higher in patients than in the healthy population. Proteomic analysis showed the upregulation of gammaIFN and IL1α, and down-regulation of OSM, NF-kB, LIF, and RXRA signaling resulted activated in this patients. Conclusion: These data demonstrate, for the first time that HIV infection induces the production of extracellular vesicles containing mediators that possibly feed the chronic inflammation and the viral replication. These two effects are connected as the inflammation itself induces the viral replication. We, therefore, hypothesize that HIV infection inhibits the production of extracellular vesicles that carry anti-inflammatory molecules.

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Increasing procoagulant activity of circulating microparticles in patients living with HIV

Cited by 7 publications

References 38 publications

Circulating microparticles in patients with chronic hepatitis C and changes during direct-acting antiviral therapy

Circulating microparticles in patients with chronic hepatitis C and changes during direct-acting antiviral therapy

Circulating Microparticles are increased amongst people presenting with HIV and advanced immune suppression in Malawi and correlate closely with arterial stiffness

Circulating extracellular vesicles as new inflammation marker in HIV infection

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