Increasing protective activity of genistein by loading into transfersomes: A new potential adjuvant in the oxidative stress-related neurodegenerative diseases?

Langasco, Rita; Fancello, Silvia; Rassu, Giovanna; Cossu, Massimo; Cavalli, Roberta; Galleri, Grazia; Giunchedi, Paolo; Migheli, Rossana; Gavini, Elisabetta

doi:10.1016/j.phymed.2018.09.207

Cited by 39 publications

(20 citation statements)

References 34 publications

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“…These may have a positive effect on NPs uptake; indeed, as reported in our previous work, spherical-shaped particles have a higher probability of entering the cancer cells [3]. Surface charge also plays an important role in cellular accumulation; several research papers described how a negative charge can improve the NPs uptake in non-phagocytic cells such as Caco-2 cells [45,46]. Unloaded NPs [3] were characterized by a surface negative electrical charge; furthermore, literature data show that polymeric nanosystems encapsulating ICG had a negative zeta potential [47,48].…”

Section: Discussionsupporting

confidence: 59%

Indocyanine Green Loaded Polymeric Nanoparticles: Physicochemical Characterization and Interaction Studies with Caco-2 Cell Line by Light and Transmission Electron Microscopy

et al. 2020

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Biomedical applications of nanoparticles (NPs) have reached an increasing development in recent years. Recently, we demonstrated that newly synthesized poly (ethyl 2-cyanoacrylate) nanoparticles (PECA-NPs) are possible antitumor agents due to their cytotoxicity for cancer cells. Indocyanine green (ICG), an amphiphilic tricarbocyanine fluorescent dye, is widely used for the detection of tumoral extension in different organs during clinical surgery. Moreover, this fluorescent agent is unstable and it has a rapid clearance in physiological conditions in vivo. In this study, ICG was charged in PECA-NPs to improve its aqueous stability and make easier its use for the identification of tumor cells. Microscopic and ultrastructural aspects concerning the related in vitro interactions between ICG-loaded NPs and tumor cell culture were investigated. Obtained results showed an effective stabilization of ICG; furthermore, color inclusions inside the cells treated with ICG-loaded NPs demonstrated the internalization of NPs with associated ICG. Transmission electron microscopy (TEM) analysis demonstrated the cytoplasmic presence of coated vesicles (Ø ≤ 100 nm), hypothesizing their involvement in the mechanism of endocytosis. Therefore, ICG-loaded NPs could be proposed as agents for tumor diagnosis, hypothesizing also in the future a specific therapeutic treatment.

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Section: Discussionsupporting

confidence: 59%

Indocyanine Green Loaded Polymeric Nanoparticles: Physicochemical Characterization and Interaction Studies with Caco-2 Cell Line by Light and Transmission Electron Microscopy

et al. 2020

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“…One of the possible permeation mechanism of the systems can be attributed to the opening of epithelial tight junctions [59] but it cannot be excluded that nanoparticles improve GEN uptake from cells of mucosa as was previously demonstrated by Langasco et al (2019) [32].…”

Section: Resultsmentioning

confidence: 99%

“…Both MTT and trypan blue analysis showed that all formulations examined did not induce changes in PC12 cells viability ( p > 0.05). The concentration of GEN was chosen on the basis of the experiments performed in a previous publication [32]. Although nanoparticles pH ranged between 4.85 and 5.63 they did not show decrease in cell viability; this is an important feature as the pH plays a fundamental role in the solubility of chitosan and thus on the stability of nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

“…PC12 cells were treated for 24 h with two series of chitosan nanoparticles (NPAc2 and NPEt1) containing GEN 30 µM, prepared in acetone and ethanol, respectively. The amount of GEN was chosen on the basis of our previous work: it was demonstrated that GEN 30 µM do not induce cytotoxicity; on the contrary, increasing the GEN concentration determines the decreasing PC12 cell vitality [32].…”

Section: Methodsmentioning

confidence: 99%

“…Although the nose-to-brain pathway proved to be useful for a variety of active compounds, the amount of drug accesses the brain is reported to be low, normally less than 0.1% [29], because of the site of administration (mucociliar clearance, drug degradation) [30,31]. Our recent work demonstrated that nanosized carriers are able to improve GEN internalization in PC12 cells, reducing ROS and the amount of apoptotic cells generated by H 2 O 2 treatment, strengthening the neuroprotective activity of GEN [32]. Among different strategies to overcome the nasal mucosal barrier, polymeric nanoparticles have attracted great attention because they can efficiently deliver a wide range of therapeutic compounds; particularly, the employment of mucoadhesive polymers can increase nasal residence and the possibility of controlled and prolonged effects on drug release and intracellular uptake [33,34].…”

Section: Introductionmentioning

confidence: 99%

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Intranasal Delivery of Genistein-Loaded Nanoparticles as a Potential Preventive System against Neurodegenerative Disorders

et al. 2018

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Genistein has been reported to have antioxidant and neuroprotective activity. Despite encouraging in vitro and in vivo results, several disadvantages such as poor water solubility, rapid metabolism, and low oral bioavailability limit the clinical application of genistein. The aim of this study was to design and characterize genistein-loaded chitosan nanoparticles for intranasal drug delivery, prepared by the ionic gelation technique by using sodium hexametaphosphate. Nanoparticles were characterized in vitro and their cytotoxicity was tested on PC12 cells. Genistein-loaded nanoparticles were prepared, and sodium hexametaphosphate was used as a valid alternative to well-known cross-linkers. Nanoparticle characteristics as well as their physical stability were affected by formulation composition and manufacturing. Small (mean diameters of 200–300 nm) and homogeneous nanoparticles were obtained and were able to improve genistein penetration through the nasal mucosa as compared to pure genistein. Nanoparticle dispersions showed a pH consistent with the nasal fluid and preserved PC12 cell vitality.

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The influence of phytoestrogens on different physiological and pathological processes: An overview

Petrine¹,

Bianco-Borges²

2020

Phytotherapy Research

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Functional foods have nutritional properties and organic functions, which are beneficial to health. Certain types of functional food components are so‐called phytoestrogens, non‐steroidal compounds derived from the metabolism of precursors contained in plants, which originate secondary metabotypes known to induce biological responses and by mimicry or modulating the action of endogenous estrogen. These molecules are involved in several physiological and pathological processes related to reproduction, bone remodeling, skin, cardiovascular, nervous, immune systems, and metabolism. This review aimed to present an overview of phytoestrogens regarding their chemical structure, actions, and effects in the organism given several pathologies. Several studies have demonstrated beneficial phytoestrogen actions, such as lipid profile improvement, cognitive function, menopause, oxidative stress, among others. Phytoestrogens effects are not completely elucidated, being necessary future research to understand the exact action mechanisms, whether they are via estrogen receptor or whether other hidden mechanisms produce these effects. Thus, this review makes a general approach to the phytoestrogen actions, beneficial effects, risk and limitations. However, the complexities of biological effects after ingestion of phytoestrogens and the differences in their metabolism and bioavailability indicate that interpretation of either risk or benefits needs to be made with caution.

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Increasing protective activity of genistein by loading into transfersomes: A new potential adjuvant in the oxidative stress-related neurodegenerative diseases?

Cited by 39 publications

References 34 publications

Indocyanine Green Loaded Polymeric Nanoparticles: Physicochemical Characterization and Interaction Studies with Caco-2 Cell Line by Light and Transmission Electron Microscopy

Indocyanine Green Loaded Polymeric Nanoparticles: Physicochemical Characterization and Interaction Studies with Caco-2 Cell Line by Light and Transmission Electron Microscopy

Intranasal Delivery of Genistein-Loaded Nanoparticles as a Potential Preventive System against Neurodegenerative Disorders

The influence of phytoestrogens on different physiological and pathological processes: An overview

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