Increasing the trapping mass range to m/z=109—A major step toward high resolution mass analysis of intact RNA, DNA and viruses

Abstract

This work demonstrates sampling of singly-charged particles up to 200 nm in diameter at atmospheric pressure into vacuum and trapping large numbers (>106) at a point in front of the end cap electrode of a linear quadrupole ion guide/trap for on-demand injection into the acceleration region of a time-of-flight mass spectrometer in a well-collimated ion packet. This procedure was shown to yield trapping efficiencies that ranged from 4–5 percent for 10 nm diameter urea particles (~ 400 kDa) to 1 percent for 200 nm urea particles (~ 3 × 109 Da). Analysis of the inlet optimization procedure suggests that the inlet can be adapted to sample and trap beyond the 200 nm range. Review of the most likely places for ion loss in the sampling process suggests that the sampling and trapping efficiencies can be improved well beyond the 4–5 percent shown. Moreover, it suggests that sampling of smaller than 10 nm ions could achieve efficiencies in the 10’s of percent range thereby suggesting new levels of sensitivity can be achieved for small ions (< 200 kDa). Finally, demonstration of trapping large numbers of 200 nm (3 × 109 Da) ions for on-demand ejection in well collimated temporally discrete ion packets is a prelude to resolved mass analysis in that range.

MS/MS networking guided analysis of molecule and gene cluster families

“…Second, our understanding of biosynthetic pathways and the function of specific enzymes found in gene clusters-especially for complex peptides made by nonribosomal peptide synthetases (NRPSs)-has dramatically increased (11)(12)(13)(14)(15)(16)(17)(18)(19). Third, the last decade has seen very significant advances in MS with respect to ion sources and the sensitivity of the instruments themselves (20)(21)(22)(23)(24)(25)(26)(27). Ambient ionization methods combined with significant improvements in sensitivity and mass accuracy of MS instrumentation now enable the detection of intact molecules directly from surfaces (7,(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40).…”

Digital mass filter analysis in stability zones A and B

“…The duty cycle axial ejection process was first modeled by our group using an ion trajectory program (SIMION, http://www.sisweb.com). The axil trapping and ejection process was also experimentally demonstrated by capturing millions of singly charged aerodynamically sized urea particles that ranged in size up to 200 nm (approximately 3 × 10 9 Da) . Our group demonstrated the agility of DWT by isolating large‐protein ions in narrow mass windows without significant ion loss .…”

“…1 The axil trapping and ejection process was also experimentally demonstrated by capturing millions of singly charged aerodynamically sized urea particles that ranged in size up to 200 nm (approximately 3 × 10 9 Da). 2 Our group demonstrated the agility of DWT by isolating large-protein ions in narrow mass windows without significant ion loss. 3 This same methodology was used to preconcentrate specific low-abundance ions to saturation for subsequent analysis in the same study.…”

Линейная Ионная Ловушка С Детерминированным Напряжением Общего Вида

“…В настоящее время ионные ловушки являются уни-версальным инструментом для пространственной лока-лизации широкого класса объектов от единичных ионов и кластеров до органических соединений [1][2][3]. Все началось в 1912 г., когда будущий нобелевский лауреат Дж.…”

“…Последние обстоятельства открывают новые возможности при реализации устройств на основе единичных ионов -квантовые стандарты частоты, квантовые процессоры. DOI: 10.21883/JTF.2017.04.44323.1920 Введение В настоящее время ионные ловушки являются уни-версальным инструментом для пространственной лока-лизации широкого класса объектов от единичных ионов и кластеров до органических соединений [1][2][3]. Все началось в 1912 г., когда будущий нобелевский лауреат Дж.…”