IncX3 Epidemic Plasmid Carrying
 bla
 NDM-5
 in Escherichia coli from Swine in Multiple Geographic Areas in China

Ho, Pak‐Leung; Wang, Ya; Liu, Chunjiao; Lai, Eileen L.; Law, Pierra Y.; Cao, Huiluo; Chow, Kin-Hung

doi:10.1128/aac.02295-17

Cited by 53 publications

(45 citation statements)

References 17 publications

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“…The IncX3-type plasmid appears to be an efficient vector for carbapenemase genes, as it has been reported to carry the bla NDM , bla OXA-48 -like, and bla KPC genes (25)(26)(27) and can disseminate the genes both inside (28) and outside (29,30) of the clinical setting. IncX3 plasmids also harbor the hns gene, a homolog of which was reported to allow plasmids to be transferred to a bacterial host by minimizing their fitness cost (31).…”

Section: Discussionmentioning

confidence: 99%

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Sugawara

Akeda

Hagiya

et al. 2019

Antimicrob Agents Chemother

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The spread of carbapenemase-producing Enterobacteriaceae (CPE), contributing to widespread carbapenem resistance, has become a global concern. However, the specific dissemination patterns of carbapenemase genes have not been intensively investigated in developing countries, including Myanmar, where NDM-type carbapenemases are spreading in clinical settings. In the present study, we phenotypically and genetically characterized 91 CPE isolates obtained from clinical (n = 77) and environmental (n = 14) samples in Yangon, Myanmar. We determined the dissemination of plasmids harboring genes encoding NDM-1 and its variants using whole-genome sequencing and plasmid analysis. IncFII plasmids harboring blaNDM-5 and IncX3 plasmids harboring blaNDM-4 or blaNDM-7 were the most prevalent plasmid types identified among the isolates. The IncFII plasmids were predominantly carried by clinical isolates of Escherichia coli, and their clonal expansion was observed within the same ward of a hospital. In contrast, the IncX3 plasmids were found in phylogenetically divergent isolates from clinical and environmental samples classified into nine species, suggesting widespread dissemination of plasmids via horizontal transfer. Half of the environmental isolates were found to possess IncX3 plasmids, and this type of plasmid was confirmed to transfer more effectively to recipient organisms at a relatively low temperature (25°C) compared to the IncFII plasmid. Moreover, various other plasmid types were identified harboring blaNDM-1, including IncFIB, IncFII, IncL/M, and IncA/C2, among clinical isolates of Klebsiella pneumoniae or Enterobacter cloacae complex. Overall, our results highlight three distinct patterns of the dissemination of blaNDM-harboring plasmids among CPE isolates in Myanmar, contributing to a better understanding of their molecular epidemiology and dissemination in a setting of endemicity.

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Section: Discussionmentioning

confidence: 99%

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Sugawara

Akeda

Hagiya

et al. 2019

Antimicrob Agents Chemother

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“…[7][8][9] These plasmids are known to disseminate various carbapenemase genes, including bla KPC and bla NDM . 10,11 To date, only two OXA-181-producing E. coli isolates have been reported in China, namely, in Sichuan and Henan. 12,13 Here, we present the first report of the identification of a K. pneumoniae isolate harboring bla OXA-181 in China.…”

Section: Introductionmentioning

confidence: 99%

First Report of OXA-181-Producing Klebsiella pneumoniae in China

Liu

Fang

Zeng

et al. 2020

IDR

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We present here the first report of an OXA-181-producing Klebsiella pneumoniae isolated from the fecal specimen of a patient in China. The OXA-181-encoding gene bla OXA-181 was located on a 51 kb IncX3-type plasmid. Conjugation assay and wholegenome sequencing analysis revealed that this transferrable plasmid in the K. pneumoniae isolate might have originated from Escherichia coli and have the potential to mediate the spread of bla OXA-181 .

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“…In 2012, our group reported the involvement of IncX3 plasmids (represented by pNDM-HN380) in the dissemination of bla NDM-1 in multiple geographic areas in China ( Ho et al, 2012b ). Subsequently, pNDM-HN380-like plasmids carrying bla NDM-1 or variants have also been described in India, the Arabian Peninsula, Europe, and Australia ( Wailan et al, 2015 ; Petrosillo et al, 2016 ; Riazzo et al, 2017 ; Choudhury et al, 2018 ; Ho et al, 2018a ). In a recent survey of more than a thousand carbapenem-resistant Enterobacteriaceae originating from hospitals located in 25 provinces and municipalities in China, the prevalence of bla NDM was found to be 31% ( Zhang et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Highly Conjugative IncX3 Epidemic Plasmid Carrying blaNDM in Enterobacteriaceae Isolates in Geographically Widespread Areas

Tong

Chow

et al. 2018

Front. Microbiol.

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The emergence of New Delhi metallo-β-lactamase (NDM) in common enterobacterial species is a major concern for healthcare. Early reports have revealed that the spread of NDM involved diverse and heterogeneous plasmids. Recently, the involvement of a rare, IncX3 subtype plasmid has been increasingly recognized. Here, we studied the prevalence of IncX plasmid subtypes in 198 carbapenem-resistant Enterobacteriaceae, originating from a territory-wide active surveillance in Hong Kong in 2016. The complete sequences and biological features of the blaNDM-carrying plasmids were investigated. A total of 62 NDM-type, 21 OXA-48 type, 14 IMP-type, 8 KPC-type, 4 IMI-type producers, and 89 non-carbapenemase-producers were tested for presence of IncX subtypes. IncX3 (n = 60) was the most common subtype, followed by IncX4 (n = 6) and IncX1 (n = 2). The prevalence of IncX3 subtype in isolates producing NDM, other carbapenemase types and non-carbapenemase producers were 75.8, 21.3, and 3.4%, respectively (P < 0.001). An IncX3 plasmid (size ∼50 kb) was confirmed to carry blaNDM in 47 isolates of different enterobacterial species. Thirteen IncX3 plasmids originating from six healthcare regions in Hong Kong were completely sequenced. The results showed that the IncX3 plasmids carrying blaNDM share a high degree of sequence identity with a previously reported plasmid, pNDM-HN380 (GenBank accession JX104760), over the backbone and genetic load regions. A blast search further revealed the occurrence of identical or nearly identical IncX3 plasmids carrying blaNDM in other part of China, Korea, Myanmar, India, Oman, Kuwait, Italy, and Canada. Two IncX3 carrying blaNDM were investigated further. Conjugation experiments demonstrated that the IncX3 plasmids could be efficiently transferred to multiple enterobacterial species at frequencies that are comparable or higher than the epidemic IncFII plasmid carrying blaCTX-M (pHK01). In addition, efficient transfer of the NDM plasmids occurred over a range of temperatures. In conclusion, this study demonstrated the important role played by IncX3 in the dissemination of NDM and the occurrence of pNDM-HN380-like plasmids in geographically widespread areas. The high mobility of IncX3 plasmid across different enterobacterial species highlights the ability of this plasmid replicon to be an important vehicle in worldwide dissemination of NDM.

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IncX3 Epidemic Plasmid Carrying bla _NDM-5 in Escherichia coli from Swine in Multiple Geographic Areas in China

Cited by 53 publications

References 17 publications

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

<p>First Report of OXA-181-Producing <em>Klebsiella pneumoniae</em> in China</p>

Occurrence of Highly Conjugative IncX3 Epidemic Plasmid Carrying blaNDM in Enterobacteriaceae Isolates in Geographically Widespread Areas

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IncX3 Epidemic Plasmid Carrying bla NDM-5 in Escherichia coli from Swine in Multiple Geographic Areas in China

Cited by 53 publications

References 17 publications

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

<p>First Report of OXA-181-Producing <em>Klebsiella pneumoniae</em> in China</p>

Occurrence of Highly Conjugative IncX3 Epidemic Plasmid Carrying blaNDM in Enterobacteriaceae Isolates in Geographically Widespread Areas

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IncX3 Epidemic Plasmid Carrying bla _NDM-5 in Escherichia coli from Swine in Multiple Geographic Areas in China