Independence of Intestinal Epithelial Cell Turnover from Cessation of Absorption of Macromolecules (Closure) in the Neonatal Mouse, Rabbit, Hamster and Guinea Pig

Rundell, John O.; Lecce, James G.

doi:10.1159/000240445

Cited by 23 publications

(16 citation statements)

References 8 publications

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“…During the immediate neonatal period these changes in mucosal architecture are accompanied by an increase in the length and number of microvilli on villus enterocytes (8), a decline in their glycogen content (14), and a cessation of their capacity to internalise macromolecules (1 5). The latter appears to occur independently of the rate of epithelial cell replacement (16), and may be due to an exhaustion of membrane available for endocytosis (17). During the late suckling period there is also POSTNATAL DAY a gradual decline in mucosal lactase activity, and a slow rise in mucosal sucrase and maltase activities (9,18).…”

Section: Resultsmentioning

confidence: 99%

Changes in Upper Intestinal Epithelial Morphology and Kinetics in the Growing Guinea Pig

Weaver

Carrick

1989

Pediatr Res

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ABSTRACT. Weaning, the process of intestinal adaptation from milk to solid diet, demands changes in gastrointestinal function. We aimed to measure upper intestinal mucosal morphology and cytokinetics during early life, to determine whether the marked changes seen at weaning in altricial species, such as rat and mouse also occur in the precocial guinea pig. A total of 79 animals was studied. Jejunal morphology was measured by microdissection and crypt cell production rate by a metaphase arrest technique in animals at 1, 7, 14, 21, and 28 days after birth. There was a 40% decline in villus height from 986 to 576 pm during

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Section: Resultsmentioning

confidence: 99%

Changes in Upper Intestinal Epithelial Morphology and Kinetics in the Growing Guinea Pig

Weaver

Carrick

1989

Pediatr Res

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“…Several factors have been identified to alter the turnover of the enterocytes, and may therefore account for variability seen in the data set; these factors include the age of the subjects, in which several publications have reported slower enterocyte turnover in neonatal or infant pigs, guinea pigs, rats, mice, and hamsters as compared with adults (Creamer et al, 1961;Koldovsky et al, 1966;Grey, 1968;Rundell and Lecce, 1972;Al-Nafussi and Wright, 1982;Holt et al, 1983;Cremaschi et al, 1986;Fan et al, 2001;Leaphart et al, 2008). In addition, altered enterocyte turnover has been reported in rats subject to numerous environmental changes and disease states, including small intestinal resection, dietary changes, diabetes, and irradiation (Menge et al, 1982(Menge et al, , 1983Cheeseman, 1986;Thomson et al, 1994).…”

Section: Downloaded Frommentioning

confidence: 99%

Meta-Analysis of the Turnover of Intestinal Epithelia in Preclinical Animal Species and Humans

Darwich

Aslam

Ashcroft

et al. 2014

Drug Metabolism and Disposition

173

108

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Due to the rapid turnover of the small intestinal epithelia, the rate at which enterocyte renewal occurs plays an important role in determining the level of drug-metabolizing enzymes in the gut wall. Current physiologically based pharmacokinetic (PBPK) models consider enzyme and enterocyte recovery as a lumped first-order rate. An assessment of enterocyte turnover would enable enzyme and enterocyte renewal to be modeled more mechanistically. A literature review together with statistical analysis was employed to establish enterocyte turnover in human and preclinical species. A total of 85 studies was identified reporting enterocyte turnover in 1602 subjects in six species. In mice, the geometric weighted combined mean (WX) enterocyte turnover was 2.81 6 1.14 days (n = 169). In rats, the weighted arithmetic mean enterocyte turnover was determined to be 2.37 days (n = 501). Humans exhibited a geometric WX enterocyte turnover of 3.48 6 1.55 days for the gastrointestinal epithelia (n = 265), displaying comparable turnover to that of cytochrome P450 enzymes in vitro (0.96-4.33 days). Statistical analysis indicated humans to display longer enterocyte turnover as compared with preclinical species. Extracted data were too sparse to support regional differences in small intestinal enterocyte turnover in humans despite being indicated in mice. The utilization of enterocyte turnover data, together with in vitro enzyme turnover in PBPK modeling, may improve the predictions of metabolic drug-drug interactions dependent on enzyme turnover (e.g., mechanism-based inhibition and enzyme induction) as well as absorption of nanoparticle delivery systems and intestinal metabolism in special populations exhibiting altered enterocyte turnover.

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“…IgG was separated from the IgG-rich fractions by chromatography on DEAE-cellulose using 0.02 m phosphate buffer, pH 8, for equilibration and elution [15]; the albumen was further purified by chromatography using 0.02 m acetate buffer for equilibrium and elution [17], followed by gel filtration on Sephadex G-100. The IgG and albumen were then radioactively labelled with 131I, and 125I, respective ly [16] and the radioactivities of all experimental samples determined by means of a Panax double-channel scintillation counter.…”

Section: Radioactively Labelled Proteinsmentioning

confidence: 99%

Studies <i>in vivo</i> and <i>in vitro</i> of the Transfer of Rat IgG and Rat Albumen Across the Intestinal Walls of Young Rats

Jones¹

1974

Neonatology

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Preparations of radioactively labelled rat albumen and IgG have been fed together in equal concentrations to 3-, 14- and 24-day-old rats. Quantitative experiments showed that both proteins were absorbed at 3 days of age, but by the 14th day, the transmission of rat albumen had ceased. No proteins were absorbed by 24-day-old rats. Gel filtration studies of the intra-lumenal contents collected 30 min after feeding have demonstrated clear differences in the relative gastric digestion of the administered proteins. In marked contrast to the in vivo studies, it has been shown that everted intestinal sacs prepared from 14- and 24-day-old rats are able to transport both rat albumen and IgG in vitro, significantly more of the former than of the latter protein being transferred under the given experimental conditions.

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Independence of Intestinal Epithelial Cell Turnover from Cessation of Absorption of Macromolecules (Closure) in the Neonatal Mouse, Rabbit, Hamster and Guinea Pig

Cited by 23 publications

References 8 publications

Changes in Upper Intestinal Epithelial Morphology and Kinetics in the Growing Guinea Pig

Changes in Upper Intestinal Epithelial Morphology and Kinetics in the Growing Guinea Pig

Meta-Analysis of the Turnover of Intestinal Epithelia in Preclinical Animal Species and Humans

Studies <i>in vivo</i> and <i>in vitro</i> of the Transfer of Rat IgG and Rat Albumen Across the Intestinal Walls of Young Rats

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