2017
DOI: 10.1073/pnas.1615395114
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Independent active and thermodynamic processes govern the nucleolus assembly in vivo

Abstract: Membraneless organelles play a central role in the organization of protoplasm by concentrating macromolecules, which allows efficient cellular processes. Recent studies have shown that, in vitro, certain components in such organelles can assemble through phase separation. Inside the cell, however, such organelles are multicomponent, with numerous intermolecular interactions that can potentially affect the demixing properties of individual components. In addition, the organelles themselves are inherently active… Show more

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Cited by 104 publications
(103 citation statements)
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“…What is notable about binary-or pseudo-binary-mixtures is that changing the concentration within the dome will not affect the compositions of the coexisting liquids, and will only alter the partition constant, λ. In biological systems, examples of UCST have been observed in vitro for the low-complexity domain of the RNA binding protein FUS [56], a disordered Nauge protein [57], and lipid bilayers [58], and in vivo for the nucleolar proteins Fibrillarin, Nopp140, RNA polymerase I, and Pitchoune [59].…”
Section: A Thermodynamics Of Phase Separationmentioning
confidence: 99%
“…What is notable about binary-or pseudo-binary-mixtures is that changing the concentration within the dome will not affect the compositions of the coexisting liquids, and will only alter the partition constant, λ. In biological systems, examples of UCST have been observed in vitro for the low-complexity domain of the RNA binding protein FUS [56], a disordered Nauge protein [57], and lipid bilayers [58], and in vivo for the nucleolar proteins Fibrillarin, Nopp140, RNA polymerase I, and Pitchoune [59].…”
Section: A Thermodynamics Of Phase Separationmentioning
confidence: 99%
“…Its recruitment during telophase is accompanied by the resumption of transcription. Work from the Wieschaus Lab using Drosophila and our work in human cells indicate that an active process centered on rDNA, namely transcription, is required for DFC formation . In human cells, mitotic bookmarking by UBF is a prerequisite for nucleolar formation.…”
Section: A Model For Nucleolar Formation In Human Cellsmentioning
confidence: 75%
“…S1A) and similar dynamics during granule fusion (Wang, Smith et al 2014). Active and passive liquid-like phases have also been described in nucleoli and stress granules, which also solidify when extruded from cells (Brangwynne, Mitchison et al 2011, Jain, Wheeler et al 2016, Falahati and Wieschaus 2017. Active scaffolds may therefore be a common underlying feature of RNA granules.…”
mentioning
confidence: 85%