SUMMARYA total of 241 group A rotavirus-positive stool samples collected from diarrhoeic patients in Thailand between July 1988 and June 1991 were characterized for their serotypes by enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies and by a polymerase chain reaction (PCR). In July 1988-June 1989, serotype 1 was the most prevalent (63-4%), followed by serotype 4 (1IO0%) and serotype 2 (8-5 %). In July 1989-June 1990, 59-8 % were serotype 1, 24-3 % were serotype 2, and 6 1 % were serotype 3. In contrast, in July 1990-June 1991, serotype 3 was detected in the highest frequency (40-5 %), 29-9 % were serotype 1, and 27-3 % were serotype 2. Thus, a distinct yearly change of serotype distribution of rotavirus in Thailand was observed in the three consecutive years. In particular, it was of note that the prevalence of serotype 3 greatly increased, in contrast to the previous studies in which almost no serotype 3 rotaviruses were detected in the years 1983-8 in Thailand.Rotavirus is a major cause of diarrhoea in infants and young children worldwide [1,2]. The mortality rate in rotavirus diarrhoea is high in areas where therapy for dehydration is not sufficiently available. Because of the importance of this disease as a major global health problem, the World Health Organization considers the development of an effective rotavirus vaccine to have a high public health priority. However, the antigenic complexity of rotavirus has hampered the development of an effective vaccine [1,3]. Epidemiological studies on distribution of human rotavirus serotypes provide the basis for interpreting the results of field trials of candidate vaccines.