Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency

Kopylchuk, G. P.; Nykolaichuk, Ivanna; Lylyk, I. S.; Bioresources, Chernivtsi

doi:10.15407/ubj92.01.113

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Після чотиритижневого утримання тварин на експериментальній дієті моделювали гостре токсичне ураження ацетамінофеном. Введення токсину здійснювали з розрахунку 1250 мг/кг маси тварини у вигляді суспензії в 2 % розчині крохмального гелю один раз на день протягом 2 діб за допомогою спеціального зонда (Kopylchuk et al, 2020)…”

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Markers of Inflammation in Rats Under Toxic Injury Against Dietary Protein Deficiency

Kopylchuk,

Nykolaichuk,

Nikorych

2023

Biolohichni systemy

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The paper presents studies of biomarkers of the development of inflammatory reactions in the blood serum of rats under the conditions of toxic damage with acetaminophen against the background of dietary protein deficiency. The animals consumed a semi-synthetic diet during the experiment according to the recommendations of the American Institute of Nutrition. In order to simulate alimentary protein deprivation, rats received a low-protein diet containing 1/3 of the standard daily protein requirement daily for 28 days. The animals were modeled acute toxic damage with acetaminophen after four weeks of experimental diet. The administration of the toxin was carried out at doses of 1250 mg/kg animal body weight in suspension in 2 % starch gel solution once a day for 2 days by gavage. Determination of the level of C-reactive protein, procalcitonin, tumor necrosis factor-alpha, interleukin-6 in the blood serum of rats was carried out by the method of immunoenzymatic analysis. We have established that toxic damage by the drug xenobiotic - acetaminophen against the background of dietary protein deficiency is accompanied by a maximum increase in the level of C-reactive protein (15.5 times) and procalcitonin (10 times) in the blood serum of rats compared to the control value, which can be considered as prognostic biomarkers of the systemic inflammatory reaction under these experimental conditions. At the same time, under these experimental conditions, hyperproduction of tumor necrosis factor-alpha and interleukin-6 was registered in the blood serum of experimental groups of rats with maximum values when toxic doses of acetaminophen were administered to protein-deficient animals, which is consistent with changes in the level of C-reactive protein and procalcitonin. The fact we found makes it possible to assume that dietary protein deprivation increases the production of TNF-α and IL-6 as pro-inflammatory mediators in toxic liver damage, thus inducing primary damage to liver parenchymal cells..

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Markers of Inflammation in Rats Under Toxic Injury Against Dietary Protein Deficiency

Kopylchuk,

Nykolaichuk,

Nikorych

2023

Biolohichni systemy

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“…Тривалість експерименту становила 28 діб. Мітохондріальну та цитозольну фракції клітин нирок щурів отримували методом диференційного центрифугування (Kopylchuk et al, 2020). Активність піруватдегідрогеназного комплексу визначали за методом (Hinman, Blass, 1981), який базується на реакції окисного декарбоксилювання пірувату з од-ночасним відновленням NAD+, що реєструють спектрофотометрично при 340 нм.…”

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Pyruvate Dehydrogenase Complex Activity in the Rat`s Kidneys Under Conditions of Toxic Acetaminophen Damage With Protein Deficiency

Voloshchuk

Chereliuk

2022

Biolohichni systemy

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The aim of this work was to study the research the enzymatic activity of pyruvate dehydrogenase complex and lac-tate dehydrogenase and determination of the lactate/pyruvate ratio in rat kidneys under conditions of toxic damage by acetaminophen against the background of protein deficiency.The activity of the pyruvate dehydrogenase complex activity was determined according to a method based on the reaction of oxidative decarboxylation of pyruvate with simultaneous reduction of NAD+, which is measured spectrophotometrically at 340 nm. LDH activity was studied by an optimized optical method, which is based on the reaction of conversion of pyruvate to lactate with concomitant oxidation of NADH, which was measured using a spectrophotometer at λ = 340 nm. Lactate content was measured spectrophotometrically by the ability of FeCl3 to interact with lactate ions, resulting in the formation of a bright yellow iron lactate solution, which is recorded at a wavelength of 390 nm. The concentration of pyruvate was deter-mined by the modified Umbright method, which is based on the reaction of the interaction of pyruvate with 2,4-Dinitrophenylhydrazine (2,4-DNPH) in an alkaline medium, during which hydrazine is formed, which was recorded using a photoelectrocolorimeter at λ = 440 nm. The study was conducted on 4 groups of experimental animals: I - control animals (K); II – rats kept on a low-protein diet (LPD); ІІІ – animals in which acute toxic damage was caused by acetaminophen (TI); IV – rats on a low-protein diet, simulated acute toxic damage with acetaminophen (LPD/TI). It is demonstrated that under the conditions of dietary protein deficiency in the kidneys, there is a decrease in the activi-ty of the pyruvate dehydrogenase complex while maintaining the control indicators of the lactate/pyruvate ratio and lactate dehydrogenase activity. However, under the conditions of the toxic effect of acetaminophen against the back-ground of dietary protein deficiency in the kidneys, an intensification of the anaerobic pathway of energy supply is observed, as evidenced by a significant decrease in the activity of the pyruvate dehydrogenase complex, an increase in the lactate/pyruvate ratio against the background of lactate dehydrogenase activation. The obtained results can be used to justify approaches to correct energy deficit in animals under the conditions of acetaminophen overdose against the background of protein deficiency.

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The Origin and Metabolism of Tryptophan

Miao,

Hu,

et al. 2024

Tryptophan in Animal Nutrition and Human Health

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Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency

Cited by 7 publications

References 25 publications

Markers of Inflammation in Rats Under Toxic Injury Against Dietary Protein Deficiency

Markers of Inflammation in Rats Under Toxic Injury Against Dietary Protein Deficiency

Pyruvate Dehydrogenase Complex Activity in the Rat`s Kidneys Under Conditions of Toxic Acetaminophen Damage With Protein Deficiency

The Origin and Metabolism of Tryptophan

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