Indication of bioactive candidates among body volatiles of gregarious adult locusts Locusta migratoria manilensis by electroantennography (EAG) test

Li, Jia; Zhang, Long

doi:10.5897/ajb11.1098

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…These cells have not been found in insects except in our previous studies as contaminated samples in L. migratoria and conceivably haemocytes in B. nigroptera . 24 , 39 In this study, many species contained megakaryocytes with relatively low abundance. Because of the limited cytoplasm and absence of specialised inclusions, the capacity of megakaryocytes to synthesise specific enzymes or peptides is low.…”

Section: Discussionmentioning

confidence: 65%

Haemocyte variations in 35 species of grasshoppers and locusts

Zhang

2021

Science Progress

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Introduction: Grasshoppers and locusts are widely distributed worldwide, causing significant losses in agriculture. The origin and functions of their haemocytes are not entirely understood. Objectives: Insect haemocytes arbitrate cellular defence and participate in humoral defences. Due to their importance, the haemocytes of 35 species of grasshoppers and locusts from China were morphologically examined in this study. We aim to highlight a simple method for the morphological examination of insect haemocytes. Methods: The haemocytes were observed, counted and compared under a light microscope after Wright-Giemsa staining. Results: High complexity in form and shape were observed in the haemocytes. These include prohaemocytes, plasmatocytes, granulocytes, vermicytes, podocytes and megakaryocytes. No clear relationship was seen between the haemocyte type and their phylogenetic relationship among the three families examined. The high abundance of plasmatocytes and granulocytes suggests their importance in the immunity of grasshoppers and locusts. The minor haemocyte populations including prohaemocytes, vermicytes and podocytes may not be always present in individuals. Conclusion: All examined species shared similarities in their haemocyte types. Wright-Giemsa staining is a simple and efficient method for evaluating haemocytes.

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Section: Discussionmentioning

confidence: 65%

Haemocyte variations in 35 species of grasshoppers and locusts

Zhang

2021

Science Progress

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“…To extend our knowledge of OR genes expressed in S. americana (Same) we analyzed the transcriptomes of the antennae and the palps. Because female and male locusts are sexually dimorphic and show some different behaviors 32 , 33 , we separately analyzed tissue from adult females and males. All together, we identified 179 transcripts of putative ORs (named here as SameORs) expressed in the two main olfactory organs.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of odorant receptors expressed in the two main olfactory organs, antennae and palps, of Schistocerca americana

Boronat-García

Iben

Domínguez-Martín

et al. 2022

Sci Rep

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Locusts depend upon their sense of smell and provide useful models for understanding olfaction. Extending this understanding requires knowledge of the molecular and structural organization of the olfactory system. Odor sensing begins with olfactory receptor neurons (ORNs), which express odorant receptors (ORs). In insects, ORNs are housed, in varying numbers, in olfactory sensilla. Because the organization of ORs within sensilla affects their function, it is essential to identify the ORs they contain. Here, using RNA sequencing, we identified 179 putative ORs in the transcriptomes of the two main olfactory organs, antenna and palp, of the locust Schistocerca americana. Quantitative expression analysis showed most putative ORs (140) are expressed in antennae while only 31 are in the palps. Further, our analysis identified one OR detected only in the palps and seven ORs that are expressed differentially by sex. An in situ analysis of OR expression suggested ORs are organized in non-random combinations within antennal sensilla. A phylogenetic comparison of OR predicted protein sequences revealed homologous relationships among two other Acrididae species. Our results provide a foundation for understanding the organization of the first stage of the olfactory system in S. americana, a well-studied model for olfactory processing.

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Electroantennographic activity of 21 aliphatic compounds that bind well to a locust odorant‐binding protein

Zhang

2022

Arch Insect Biochem Physiol

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Odorants that bind well to odorant‐binding proteins (OBPs) often trigger olfactory responses and have important biological significance. The locust Locusta migratoria (Meyen) (Orthoptera: Acrididae) is a serious agricultural pest. Twenty‐one saturated aliphatic compounds with carbon–oxygen bonds and straight chains of 10–17 carbon atoms bind well to an L. migratoria OBP. In this study, olfactory activities of these aliphatic compounds on L. migratoria adult males were tested by electroantennography (EAG) and comparatively analyzed. Four alcohols (undecanol, dodecanol, tridecanol, and tetradecanol), two ketones (2‐dodecanone and 2‐tridecanone), and two esters (ethyl octanoate and ethyl nonanoate) triggered strong EAG responses, and there was no significant difference between them. The results suggest that the eight compounds are more likely to have important biological significance than the other compounds. Moreover, we found that there is not necessarily a positive correlation between the olfactory activity of odorants and their binding ability with OBP. The study contributes to understanding the odorants with biological significance for L. migratoria and the molecular mechanism of the locust's olfaction.

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Indication of bioactive candidates among body volatiles of gregarious adult locusts Locusta migratoria manilensis by electroantennography (EAG) test

Cited by 6 publications

References 26 publications

Haemocyte variations in 35 species of grasshoppers and locusts

Haemocyte variations in 35 species of grasshoppers and locusts

Identification and analysis of odorant receptors expressed in the two main olfactory organs, antennae and palps, of Schistocerca americana

Electroantennographic activity of 21 aliphatic compounds that bind well to a locust odorant‐binding protein

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