Indicators of Sorption Ability and Permeability of Erythrocyte Membranes in Children and Newborns, Living in the Area of Increased Radiation Background

Badalova, Zebo Abdulkhayrovna; Додхоев, Д. С.

doi:10.25005/2074-0581-2019-21-4-597-602

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…The χ2 (Pearson) criterion was used to assess the statistical significance of the differences between two or more relative data. Group differences were considered statistically significant at p-value <0.05 [1].…”

Section: T a B L Ementioning

confidence: 99%

Influence of cardiopulmonary bypass on the erythrocyte membranes and the method of its protection

et al. 2021

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The damage to erythrocytes during cardiopulmonary bypass (CPB) remains a recent problem. The aim of this research was to study the effect of fructose-1,6-diphosphate on the state of the erythrocyte membrane during CPB and the level of phosphorus in blood as a marker of the energy potential in the cell. Patients were divided into two groups. The control group 1 (Gr 1) consisted of 75 individuals. The group 2 (Gr 2) included patients to whom fructose-1,6-diphosphate (FDP) was administrated according to the developed scheme as follows 10 g of the drug was diluted in 50 ml of a solvent, 5 g of the drug was injected intravenously with the use of perfusor immediately before initiation of CPB at a rate of 10 ml/min and 5 g at the 30th minute of CPB (before the stage of warming) the same way. When comparing two groups the best results in hemolysis (p<0.01), mechanical (p<0.01). osmotic resistance of erythrocytes (p<0.01), the time of acid hemolysis (p<0.01) and the permeability of the erythrocyte membrane in postperfusion period were in Gr 2. Вefore cardiac surgery hypophosphatemia was detected in 18% out of 150 and in 32% out of 150 patients – a lower limit of normal phosphorus content in the blood. After CPB in Gr 1 phosphorus content in blood was 0.85±0.32 mmol/l and hypophosphatemia was in 53% out of 75 patients. This indicates a pronounced energy deficit in this group. In Gr 2 phosphorus level was 1.7±0.31 mmol/l and there was no hypophosphatemia. As a result, FDP as an endogenous high-energy intermediate metabolite of the glycolytic pathway leads to resistance to hemolysis, protects the erythrocyte membrane from damage and increases the energy potential of the cell during CPB.

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Section: T a B L Ementioning

confidence: 99%

Influence of cardiopulmonary bypass on the erythrocyte membranes and the method of its protection

et al. 2021

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Protection of Red Blood Cells During Cardiopulmonary Bypass

Cherniy

Sobanska

Shestak

2021

Clin. and prev. med.

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Aim. This study was aimed at developing biocompatibility of extracorporeal components due to the use of «adaptation composition» (AdC), reducing the negative impact of perfusion on the state of red blood cell due to the use of fructose-1,6-diphosphate (FDP) to decrease intraoperative hemolysis and tissue hypoxia. Material and methods. The study included 225 patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). The first group included patients who underwent surgery with the treatment of an extracorporeal circuit by AdC, the second group included patients who were administered the drug with the active substance FDP, the third group was the control group. The oxygenator was treated with AdC and PDF was administrated according to the protocol. Patient blood was sampling for complete blood cell count (CBC) and blood smears were at 4 stages of surgery: before CPB, at 10 min. CPB-time, at 60 min. CPB-time (rewarming stage) and after separation from CPB. Several parameters were studied: plasma free hemoglobin (plfHb), the erythrocytes osmotic, mechanical resistance of erythrocytes, erythrocyte membrane permeability (EMP), acid hemolysis, the blood smears were stained according to Pappenheim. Results. The best erythrocyte indices, hemolysis, osmotic, mechanical, acid, urea resistance of erythrocytes were observed in the groups where fructose-1,6-diphosphate and «adaptation composition» were used. At the preoperative stage, hypophosphatemia was detected in 16.8% out of 225 patients and 26.6% out of 225 patients have a clear tendency to ones. After CPB, there was no hypophosphatemia in the group where fructose-1,6-diphosphate was administered. The higher peripheralization of reticulocytes, echinocytes, and spherocytes was observed in the control group after perfusion. Conclusion. The use of AdC and FDP during CPB helps to reduce hemolysis and the better state of erythrocytes. Cardiopulmonary bypass (CPB) can negatively affect the state of red blood cell. The effect of fructose-1,6-diphosphate and «adaptation composition» on the state of erythrocytes during perfusion was studied.

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Method of Erythrocyte Protection in Urgent Cardiac Surgery

Cherniy

Sobanska

2021

Emerg Med Serv

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Aim: The use of a simple, safe and convenient method of treatment extracorporeal circuit with «adaptation composition » (AdC) for the reduction of negative impact on the state of erythrocytes and tissue hypoxia. Material and methods: The research included 150 patients. They were divided into two groups. The first group included patients who underwent surgery with the treatment of an extracorporeal circuit by AdC, the second group was the control group. Сomplete blood count parameters, plasma free hemoglobin concentration, erythrocyte mechanical and osmotic resistance, erythrocyte membrane permeability and acid hemolysis were studied. Results: Use of AdC prevents adsorption the blood cells on the surface of the extracorporeal circuit and protects the erythrocytes during cardiopulmonary bypass in urgent cardiac surgery. Conclusions: The treatment of oxygenator with AdC reduces the negative influence СРВ on state of RBC. Membranes of erythrocytes were more resistant to traumatic factors in the group with AdC.

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Indicators of Sorption Ability and Permeability of Erythrocyte Membranes in Children and Newborns, Living in the Area of Increased Radiation Background

Cited by 3 publications

References 8 publications

Influence of cardiopulmonary bypass on the erythrocyte membranes and the method of its protection

Influence of cardiopulmonary bypass on the erythrocyte membranes and the method of its protection

Protection of Red Blood Cells During Cardiopulmonary Bypass

Method of Erythrocyte Protection in Urgent Cardiac Surgery

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