Indifferent and Haemolytic Streptococci Possessing Group-Antigen F

Ottens, Henk; Winkler, Kurt

doi:10.1099/00221287-28-1-181

Cited by 78 publications

(60 citation statements)

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“…Ottens & Winkler (1962) examined non-haemolytic streptococci from the dental root-canal, and found that a number of them possessed the Lancefield-group antigen C, F or G. These workers recognized that the non-haemolytic members of groups C and G differed from the familiar 'pyogenic' ,-haemolytic members of these groups, and also detected the type antigens of the group-F streptococcus (Bliss, 1937) in many of the non-haemolytic streptococci of the dental root-canal, whether or not a group antigen was present. Colman & Williams (1965, 1972; see also Colman, 1968) compared representative strains from Guthof (1956) and Ottens & Winkler (1962) with minute-colony forming f,-haemolytic streptococci of groups F and G (Long & Bliss, 1934;Bliss, 1937) and with the type strain of Streptococcus MG (Mirick et al 1944). They concluded that all of them should be included in the species S. milleri, but characterized it by a somewhat different set of biochemical characters from those given by Guthof (1956), by the sugar components in the cell wall, and by the frequent possession of one of a series of antigens that included the type antigens of group-F streptococci (the so-called Ottens antigens).…”

Section: Introductionmentioning

confidence: 99%

The cultural and biochemical characters ofStreptococcus milleristrains isolated from human sources

Ball

Parker

1979

J. Hyg.

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SUMMARYA collection of 346 strains of Streptococcus milleri from a variety of human sources was examined culturally and biochemically, and for the presence of Lancefield group antigens. Most of the strains were non-haemolytic and ungroupable, but 25 % were fi haemolytic and 19 % were a haemolytic; 28 % possessed a group antigen (A, 5 %; C, 6 %, F, 14 %, G, 3 %). These antigens were present in 69 % of f8-haemolytic but in only 13 % of a-haemolytic or non-haemolytic strains; fi haemolysis occurred in 82 % of group-F strains, 43 % of other groupable strains and 11 % of ungroupable strains.The following reactions were given by > 80 % of S. milleri strains: hydrolysis of arginine and aesculin, a positive Voges-Proskauer reaction, and acidification of trehalose, lactose, salicin and sucrose. A minority of strains showed enhancement of growth by CO2, bile tolerance, NaCl tolerance, and ability to acidify other sugars, notably mannitol, raffinose and melibiose. Departures from the modal pattern of biochemical reactions showed a weak correlation with the type of haemolysis and the presence or absence of a group antigen but were not sufficiently systematic for clear-cut subdivisions to be recognized within the species. S. milleri therefore appeared to comprise a 'central' group of non-haemolytic strains that rarely formed a Lancefield-group antigen, in which the aesculin reaction was nearly always positive, lactose was usually acidified, and a considerable minority showed enhancement of growth by C02 and bile tolerance.Deviations from this pattern were of two main types. (1) 'Loss' of one or more of these reactions, which tended to be associated with ,-haemolysis and presence of a group antigen. In these respects, a-haemolytic strains tended to occupy an intermediate position. (2) 'Gain' of the ability to acidify additional sugars, notably raffinose and melibiose or mannitol; this occurred mainly among otherwise typical non-haemolytic strains that were rarely groupable.Only 12 % of isolations from the bloodstream of patients suffering from systemic infections were , haemolytic and only 18 % possessed a group antigen, but a considerably greater proportion of those from visceral abscesses were fi haemolytic (28 %). Among isolations from superficial lesions in some body sites there were considerably greater proportions of f8-haemolytic and groupable strains; thus, nearly one-half of those isolated from the abdomen other than the female genital tract were f,-haemolytic and over one-half were groupable. On the other hand, strains from the teeth and gums were nearly always non-haemolytic and

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Section: Introductionmentioning

confidence: 99%

The cultural and biochemical characters ofStreptococcus milleristrains isolated from human sources

Ball

Parker

1979

J. Hyg.

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“…The P-haemolytic strains, which joined the cluster at a below-average level of similarity, differed from the non-haemolytic strains by their inability to ferment lactose. All group-F strains except one were found within the S. milleri cluster; type-I1 and type-I11 antigens (Ottens and Winkler, 1962) were exclusively present in S. milleri strains, with the exception of NCTC10446 (group T). The characteristics of the S. milleri-like strains outlined here correspond well with other descriptions (Ottens and Winkler, 1962;Colman and Williams, 1967;Edwardsson, 1974;Mejire, 1975a;Mejire and Edwardsson, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…All group-F strains except one were found within the S. milleri cluster; type-I1 and type-I11 antigens (Ottens and Winkler, 1962) were exclusively present in S. milleri strains, with the exception of NCTC10446 (group T). The characteristics of the S. milleri-like strains outlined here correspond well with other descriptions (Ottens and Winkler, 1962;Colman and Williams, 1967;Edwardsson, 1974;Mejire, 1975a;Mejire and Edwardsson, 1975). The findings of this study are consistent with the view that streptococci resembling S. milleri in their physiological properties should be so named, even if they possess a group A, C, F or G antigen, and whether or not they are p-haemolytic (Colman andWilliams, 1967, 1972;Parker and Ball, 1976;Poole and Wilson, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…No vaccine strain for type I was available at the time of this investigation; type V of Ottens and Winkler (1962) has proved not to be a distinct type upon further investigation (Willers, personal communication, 1976). The " 23 " and " 25 " (HS 8650-de Moor) strains described by Willers and Alderkamp (1967) and Willers, Michel and Benner (1973~) were also used for the production of precipitating rabbit antibodies.…”

Section: Serological Proceduresmentioning

confidence: 99%

“…Typing sera for the types 11, 111, and IV of group F (Ottens and Winkler, 1962) were produced in the usual manner by inoculating rabbits with pepsin-treated, heat-killed cells of the appropriate streptococcal reference strains obtained from Dr J. M. N. Willers, University of Utrecht, Netherlands. No vaccine strain for type I was available at the time of this investigation; type V of Ottens and Winkler (1962) has proved not to be a distinct type upon further investigation (Willers, personal communication, 1976).…”

Section: Serological Proceduresmentioning

confidence: 99%

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