Indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonas diminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

“…The enzyme is encoded by an opd (organophosphate degradation) gene and is capable of hydrolyzing a wide range of oxon and thion organophosphates [20,21]. The opd gene has been detected in soil microorganisms belonging to different taxonomic groups, which points to its horizontal spread using mobile genetic elements (transposons, plasmids, phages) as transfer vehicles [22][23][24][25][26][27][28].…”

The Organophosphate Degradation (opd) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p-Nitrophenol Degradation

“…In pPDL2, insertion of Tn3 in orf345 appears to have created orf306 and generated the genetic organization seen in this plasmid. That organization, which resembles a complex transposon, suggests possible reasons for the existence of identical opd regions in taxonomically diverse microbes (30).…”

“…The dissimilar opd plasmids pCMS1 and pPDL2 are mobilizable in nature and possess absolute sequence identity in the DNA region containing the opd gene, the IS element IS21, and orf306. However, in pCMS1, the sequence coding orf306 is extended to give an ORF coding for a 345-amino acid carboxyesterase that appears to form an operon along with an ORF coding for amidase (30). In pPDL2, insertion of Tn3 in orf345 appears to have created orf306 and generated the genetic organization seen in this plasmid.…”

Organophosphate Hydrolase Is a Lipoprotein and Interacts with Pi-specific Transport System to Facilitate Growth of Brevundimonas diminuta Using OP Insecticide as Source of Phosphate

“…OPH is encoded by the opd (organophosphate degrading) gene found on dissimilar plasmids and the opd gene has recently been shown to be a part of an integrative mobilizable element (IME) (4). Due to the mobile nature of the opd island, identical opd genes are found among bacterial strains isolated from different geographical regions (4,5). Although its physiological substrate is unknown, OPH hydrolyzes paraoxon at a rate approaching the diffusion limit (k cat /K m 10 8 M Ϫ1 s Ϫ1 ) (6).…”