Indigofera oblongifolia leaf extract regulates spleen macrophage response during Plasmodium chabaudi infection

Abstract

Malaria is a major health problem that still affects numerous countries. The current study aimed to identify the role of leaf extract in regulating mouse spleen macrophages during the progression of infection. Three doses of the leaf extract (100, 200, and 300 mg/kg) were administered to mice inoculated with infected erythrocytes. The weight of the infected mice improved after the treatment with. The infection causes disorganization of macrophage distribution in the spleen. After the mice had been treated with the leaf extract, the macrophages appeared to be reorganized in the white and red pulp areas. In addition, the leaf extract (IOLE) significantly increased the total antioxidant capacity of the mice spleens infected with. The phagocytic activity of spleen macrophages was increased in the infected group as indicated by the significant decrease in the number of fluorescent particles in the spleen sections. This number increased in the mice spleens after treatment with IOLE. Based on these results, it is suggested that IOLE regulate macrophage response of the spleen during the blood stage of malaria in mice.

Effect of Indigofera oblongifolia on the Hepatic Oxidative Status and Expression of Inflammatory and Apoptotic Genes during Blood-Stage Murine Malaria

“…I. oblongifolia leaf extract (IE) contains polyphenols, flavonoids, and organic acids [16]. IE showed antimalarial and antioxidant activities and provides protection to the spleen from the parasite P. chabaudi in mice [4–6]. In the present study, we demonstrate the role of IE in the modulation of cytokine expression and apoptosis in mouse liver infected with blood-stage malaria.…”

“…In addition, catalase degrades hydrogen peroxide (H 2 O 2 ) into H 2 O and O 2 and causes reduction in the level of ROS. Several studies have highlighted the antioxidant role of IE especially in the spleen and kidney [5, 6, 17]. In this study, the hepatic GSH level and catalase activity were significantly reduced upon infection, while treatment with IE resulted in the restoration of the GSH level and catalase activity.…”

“…In the sinusoid, sporozoites cross the sinusoidal cell layer to infect hepatocytes and grow and develop into erythrocyte-invasive forms [3]. Several studies have investigated the effector role of the liver during blood-stage malaria [4–6]. Malaria infection is associated with both acquired immune [7, 8] and innate immune [9] responses, characterized with early and intense proinflammatory cytokine-mediated effector mechanisms that kill or remove parasite-infected cells [10].…”

Biologically active isoquinoline alkaloids covering 2019–2022

“…It reduced H 2 O 2 -induced growth inhibition and DNA damage as well as apoptosis in C2C12 cells by suppressing the accumulation of intracellular reactive oxygen species via activation of the Nrf2/HO-1 pathway 297 . Additionally, the alkaloid improved the antioxidant status of intestinal tissue in mice 298 . The related alkaloid coptisine (165) exerted an antioxidant activity against AAPH-induced toxicity by activating Akt and JNK/Nrf2/NQO1 pathways 299 .…”