The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammary inoculated with 1.5×10 8 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1 st group left without treatment as control positive, 2 nd group treated intramammary infusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3 rd group treated intramammary infusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4 th group treated intramammary infusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14-and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.