Indirect ELISA for the diagnosis of brucellosis in water buffaloes (Bubalus bubalis) in Italy

Guarino, Alfredo; Fusco, Giovanna; Serpe, Luigi; Gallo, Pasquale; Matteo, Andrea Di; Urbani, G; Tittarelli, Manuela; Ventura, Mauro Di; Condoleo, Roberto

doi:10.1136/vr.149.3.88

Cited by 21 publications

(19 citation statements)

References 5 publications

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“…However, by using LPS the specificity was slightly lower than the sensitivity (99.2%) observed by Abalos et al (2000) when using the same antigen. According to Guarino et al (2001), the high percentage of positivity was due to the ability of this test to detect very low levels of antibodies present in the early stage of infection, while RBPT and SAT cannot detect it. The use of smooth LPS as antigen in the I-ELISA might be one of the reasons for higher sensitivity.…”

Section: Discussionmentioning

confidence: 96%

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Ghanem

El-Khodery

Saad³

et al. 2009

Trop Anim Health Prod

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The present study was delineated to investigate the prevalence and risk factors of camel brucellosis in Northern Somalia (Somaliland). The study was carried out at three main districts of camel-rearing regions of Somaliland (Awdal, Waqoyi Galbed and Togdheer) in the period from July to November, 2008. A total of 1246 camel blood sera were randomly collected from 42 sporadic small scale camel herds. Two serological tests were used to screen all serum samples, Rose Bengal Plate Test (RBPT) and indirect ELISA (I-ELISA). Multivariate logistic regression was constructed to study the risk factors associated with Brucella seropositive cases. The overall prevalence of camel brucellosis in districts under investigation was 3.9% by RBPT and 3.1% by (I-ELISA). Multivariate logistic regression on animal level showed that locality (P < 0.05; OR: 6.254; CI, 1.186-32.976), herd size (P < 0.001; OR: 5.493; CI, 2.956-10-207), rearing with other ruminants (P < 0.001; OR: 12.433; CI, 3.957-39.060), and contact with other camels (P < 0.05; OR: 5.311; CI, 1.093-25.800) were the potential risk factors. However, herd size (P < 0.05; OR: 5.425; CI, 1.181-24.932), and rearing with other ruminants (P < 0.05; OR: 20.466; CI, 1.456-28.638) were recorded as risk factors on the herd level. The results of the present investigation indicate that the Brucella spp. exists within the camel herds in Somaliland. Further studies need to be done on Brucella infection in the other ruminants to determine which measure should be followed for control of brucellosis.

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Section: Discussionmentioning

confidence: 96%

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Ghanem

El-Khodery

Saad³

et al. 2009

Trop Anim Health Prod

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“…Different researchers have described various reasons for the higher sensitivity of I-ELISA. According to Guarino et al (2001), the high percentage of positivity was due to the ability of this test to detect very low levels of antibodies present in the early stage of infection, while RBPT and SAT cannot detect it. The use of smooth LPS as antigen in the I-ELISA dev might be one of the reasons for higher sensitivity as the stronger immune responses are elicited against LPS in infected animals.…”

Section: Discussionmentioning

confidence: 99%

“…At present, application of the ELISA technique is considered a better test in early detection of infection than complement fixation test (Rojax and Alonso 1995). Indirect enzyme linked immunosorbent assays (I-ELISAs) have been developed and used in various countries for sero-diagnosis of brucellosis in cattle and other animals (Omer et al 2001;Dajer et al 1998;Molnar et al 1998;Romero et al 1995), however, such kind of work in buffaloes is limited (Guarino et al 2001).…”

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confidence: 99%

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Munir¹,

Rehman

Kausar³

et al. 2008

Acta Vet. Brno

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Brucellosis is an important zoonotic disease causing significant economic losses worldwide. Early detection of this disease is essential for its control and eradication. Presently, an Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) was developed using lipopolysaccharide (LPS) as antigen and compared with the commercial kit using one hundred negative and positive sera each from buffaloes. The agreement for the positive result between the developed and commercial I-ELISA was 78% and for the negative it was 100%. At 52.49%, 53.09%, 53.26%, 53.86% and 53.94% cut off the sensitivity was 100%, 100%, 97.53%, 88.93% and 86.42%, while the specificity was 84.03%, 84.87%, 85.71%, 87.39% and 87.39%, respectively, for developed I-ELISA. This developed test can be used for the screening of herds as the relative sensitivity is higher. Brucella abortus, I-ELISA, lipopolysaccharides (LPS)

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“…O sistema de manejo extensivo, o insipiente programa de controle sanitário no Brasil, os grandes rebanhos, as condições ambientais das propriedades e a crença equivocada de que a espécie é imune a algumas doenças que acometem bovinos dificultam o controle da brucelose em bubalinos (GUARINO et al, 2001). FOSGATE et al (2002) sugerem haver características epidemiológicas diferentes entre bovinos e bubalinos, ratificando a necessidade de mais estudos nesse tema, pois a maioria dos trabalhos sobre a brucelose animal refere-se à espécie bovina.…”

Section: B Cetaceae (Isolados De Cetáceos)unclassified

Estudo comparativo de diferentes técnicas sorológicas para diagnóstico de infecções por Brucella abortus em búfalos (Bubalus bubalis)

Silva¹

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Indirect ELISA for the diagnosis of brucellosis in water buffaloes (Bubalus bubalis) in Italy

Cited by 21 publications

References 5 publications

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Estudo comparativo de diferentes técnicas sorológicas para diagnóstico de infecções por Brucella abortus em búfalos (Bubalus bubalis)

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