Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Abstract

Brucellosis is an important zoonotic disease causing significant economic losses worldwide. Early detection of this disease is essential for its control and eradication. Presently, an Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) was developed using lipopolysaccharide (LPS) as antigen and compared with the commercial kit using one hundred negative and positive sera each from buffaloes. The agreement for the positive result between the developed and commercial I-ELISA was 78% and for the negative it was 100%. At 52.49%, 53.09%, 53.26%, 53.86% and 53.94% cut off the sensitivity was 100%, 100%, 97.53%, 88.93% and 86.42%, while the specificity was 84.03%, 84.87%, 85.71%, 87.39% and 87.39%, respectively, for developed I-ELISA. This developed test can be used for the screening of herds as the relative sensitivity is higher. Brucella abortus, I-ELISA, lipopolysaccharides (LPS)

Development And Validation of An In-House Brucella Smooth Lipopolysaccharide as A Coating Antigen in ELISA For Serodiagnosis of Bovine Brucellosis

“…In house S-LPS have been successfully produced. This study differs from other performed research[15][16][17][18] in that the buffer used with in-house made ELISA was not in house prepared but it is commercially prepared and validated buffers, so, this (95%-CI: 56.59-74.49%) were found positive for Brucella antibodies by in house i-ELISA, commercial i-ELISA and CFT, respectivelyAmerican Journal of Biomedical Science & Research…”

Brucellosis in human and domestic animals in Bangladesh: A review

“…Buffaloes are known to be affected with B. abortus and less frequently with B. melitensis (Munir et al, 2008;Ahmed et al, 2010). Similar to cattle, Brucella infections are known to result in late gestation (6-9 months) abortions (Sanjrani et al, 2013), infertility (Sukumar et al, 2012) and latent infection of mammary gland lymph nodes with shedding of organisms in the milk (Ahmed et al, 2010), yet abortions are less common in buffaloes (The Center for Food Security and Public Health Iowa State University, 2009) with the disease being endemic in most buffalo raising countries.…”