Indirect photometric detection in capillary zone electrophoresis

Foret, František; Fanali, Salvatore; Ossicini, L.; Boček, Petr

doi:10.1016/s0021-9673(01)83558-5

Cited by 251 publications

(88 citation statements)

References 21 publications

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“…Recently [12], we found analytic solutions for the transport of monofunctional acids or bases in buffers based on monofuncResults of the two approaches are numerically the same. Together with the results cited above [8][9][10][11][12] they yield a common result of general importance. In this work we will restrict the discussion to cases of relatively small overload, such that extreme effects as, e.g., peak splitting, described and explained by Ermakov et al [13], do not occur.…”

Section: The Electromigration Dispersion Emd Constantsupporting

confidence: 65%

“…The phenomenon, especially when caused by conductivity changes, has been investigated for non-protolyzing solutes and buffers by Mikkers et al [8] and for weak acids and bases by Foret et al [9]. The effect of pH Changes has received little attention; recently, Beckers [10] applied a numerical procedure to obtain insight into this phenomenon.…”

Section: The Electromigration Dispersion Emd Constantmentioning

confidence: 99%

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Optimum conditions for preparative operation of capillary zone electrophoresis

Cifuentes

Kok

et al. 1995

Journal of Chromatography A

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Optimum conditions for preparative operation of capillary zone electrophoresisCifuentes, A.; Xu, X.; Kok, W.T.; Poppe, H. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractThe possibilities to use capillary zone electrophoresis (CZE) as a preparative tool have been studied, mostly from a theoretical point of view. The preparative performance of CZE is quantitated by the production rate, the amount of analyte that can be purified per unit of time. The production rate available with CZE is shown to depend in the first place on the chemical characteristics of the analyte-buffer combination. A figure of merit can be defined for a buffer system, describing its susceptibility to analyte overloading. Methods to find an optimal bufer system are referred to. It is shown that the loadability of the system is inversely proportional to the plate number required for the separation.A second important factor for the maximum production rate is shown to be the thermal management of the instrumental system. The average increase of the solution temperature and the non-uniform migration velocity by temperature differences within the solutions are discussed. It is shown that in practical cases the temperature rise of the solutions will be the main limiting factor when high fields are used. With low fields, which in general give a higher production rate, siphoning of the solution becomes the dominant limitation for the production rate.With cylindrical capillaries the highest production rate that can be obtained under practical conditions is in the order of 5.10 -13 mol s -1. Experimental and theoretical results indicate that with rectangular capillaries the production rate can be increased by a factor of three.

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Section: The Electromigration Dispersion Emd Constantsupporting

confidence: 65%

Section: The Electromigration Dispersion Emd Constantmentioning

confidence: 99%

Optimum conditions for preparative operation of capillary zone electrophoresis

Cifuentes

Kok

et al. 1995

Journal of Chromatography A

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“…Often it is stated that the influence of the presence of sample components on the composition of the BGE can be neglected if the sample concentrations are at least a factor of 100 lower than that of the BGE [1]. In that case, the composition of the BGE can be considered to be constant through the 0021-9673/95/$09.50 © 1995 Elsevier Science B.V. All rights SSDI 0021-9673(94) whole system.…”

Section: Introductionmentioning

confidence: 99%

“…Component peaks often have a strong tailing or fronting character if the mobilities of the sample components differ from those of the co-ions of the BGE, whereas experimentally determined mobilities, calculated from the migration times of the top of the peaks, differ considerably from values in the literature, indicating a large influence of electrodispersive effects. Of course, these effects can be minimized by choosing a BGE with a co-ion, the mobility of which is reserved nearly equal to that of the sample component to be determined [1]. Nevertheless it is of interest to study whether arithmatic corrections can be carried out for electrodispersive effects.…”

Section: Introductionmentioning

confidence: 99%

Calculation of the composition of sample zones in capillary zone electrophoresis I. Mathematical model

Beckers

1995

Journal of Chromatography A

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“…CZE-in-UV has also been employed in the separation of amino acids. [13][14][15][16][17] Lee et al reported that p-aminosalicylic acid (PAS) and 4-(N,N-dimethylamino) benzoic acid were adopted as BGEs for the separation of 20 amino acids by CZE-in-UV. 14,15 However, its separation time required 40 min.…”

Section: Introductionmentioning

confidence: 99%

Determination of Amino Acids in Tobacco Samples by Capillary Electrophoresis/Indirect Absorbance Detection with Isolation of the Electrolysis Compartment and p-Aminobenzoic Acid as a Background Electrolyte

Yang

Wang

et al. 2006

ANAL. SCI.

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A fast, convenient and sensitive method of capillary zone electrophoresis (CZE) and indirect UV detection was proposed for the determination of 16 amino acids. p-Aminobenzoic acid (PAB) was selected as a background electrolyte (BGE). An isolated cell included a BGE buffer part and an electrode buffer one, which were jointed with a glass frit. The isolated cell can prevent PAB from the electrode reaction and improve the stability of the detection baseline. The separation conditions of amino acids were investigated, such as different BGEs, BGE concentration, buffer pH and electroosmotic flow (EOF) modifiers. Under the selected separation conditions, 14 amino acid peaks could be separated in 12 min. The detection limits of the amino acids were in the range of 1.7 -4.5 μmol/L. The isolated cell is suitable for reagents reacting on the electrodes in capillary electrophoresis. The proposed method has been successfully applied to the determination of the amino acids in tobacco samples.

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Indirect photometric detection in capillary zone electrophoresis

Cited by 251 publications

References 21 publications

Optimum conditions for preparative operation of capillary zone electrophoresis

Optimum conditions for preparative operation of capillary zone electrophoresis

Calculation of the composition of sample zones in capillary zone electrophoresis I. Mathematical model

Determination of Amino Acids in Tobacco Samples by Capillary Electrophoresis/Indirect Absorbance Detection with Isolation of the Electrolysis Compartment and p-Aminobenzoic Acid as a Background Electrolyte

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