Indirect Spectrophotometric Determination of Famotidine and Ciprofloxacin Hydrochloride in Pharmaceuticals Using N-Bromosuccinimde and Janus Green B Dye.

Abstract

An indirect simple and sensitive spectrophotometric method has been developed for the determination of famotidine (FAM) and ciprofloxacin hydrochloride (CIP) in pure and pharmaceutical dosages. The method is based on the oxidation of FAM and CIP with known excess of N-Bromosuccinimde in acidic medium and subsequent occupation of unreacted oxidant in decolorization of Janus green B dye and measure the absorbance of residual dye at 618 nm. Calibration curves of residual Janus green B dye in the presence of FAM or CIP were rectilinear over the ranges 1.0-10.0 and 1.0-7.5 µg/ml with molar absorptivity 3.61×10 4 and 5.14×10 4 l.mol -1 .cm -1 respectively. The accuracy (average recovery) was ranged between 99.68 and 100.23% and precision (RSD%) is less than 3.80%. The developed method was successfully applied for the determination of the studied drugs in their pharmaceutical preparations. The results obtained are in agreement with certified values of pharmaceutical preparations and also with standard addition procedure and standard method applied in state company for drugs industry and medical appliance,SDI.

Simultaneous Quantitative Determination of Ciprofloxacin and Hydrocortisone by H-Point Standard Addition Method

“…Figure 2. The chemical structure of hydrocortisone Some spectrophotometric methods have been reported to determine ciprofloxacin [9][10][11][12][13][14][15][16] and hydrocortisone [17][18][19][20][21][22][23]. Due to their intrinsic simplicity, sufficient sensitivity, affordability, and widespread presence in all quality control laboratories, these procedures are the most practical ones.…”

Spectrophotometric Method for the Determination of Ciprofloxacin in Pure and Pharmaceutical Preparations: Development and Validation

“…Several analytical methods have been reported for the quantification of ciprofloxacin, including high-performance liquid chromatography (HPLC) [4,5], spectrofluorometry [6], flow injection analysis [7], voltammetry [8], derivative spectrophotometric methods [9], titrimetric and spectrophotometric techniques using cerium (IV) sulfate [10], and various other spectrophotometric techniques [11,12]. Some studies have focused on the development of spectrophotometric methods based on charge-transfer complexation techniques [13], indirect spectrophotometric determination using N-Bromosuccinimide and Janus Green B dye [14], and the use of capillary electrophoresis with transient pseudo-isotachophoresis [11,12,15]. The objective of this study is to develop a spectrophotometric analytical method for the quantification of ciprofloxacin hydrochloride in pharmaceutical preparations.…”