Indiscriminate ingestion of entomopathogenic nematodes and their symbiotic bacteria by Aedes aegypti larvae: a novel strategy to control the vector of Chikungunya, dengue and yellow fever

Shah, Farooq A.; Abdoarrahem, M. M.; Berry, Colin; Touray, Mustapha; Hazır, Selçuk; Butt, Tariq M.

doi:10.3906/zoo-2107-2

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…Similarly, Ahmed et al 28 reported that the P. luminescens bacterial toxin complexes showed promising larvicidal activities against the mosquito vector, Culex pipiens. Conversely, Shah et al 62 found that X. nematophila bacterial supernatants and cell suspensions caused more than 91% larval mortality at a concentration of 50% and 48 h after treatment, while P. laumondii bacterial cell suspensions killed 75%, but bacterial supernatants were not effective at any tested concentration.…”

Section: Discussionmentioning

confidence: 97%

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

El-Rah¹,

Abdelazeem²,

Elbrense³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: The housefly poses a threat to the public health of humans and domestic animals since it can carry and transmit pathogens. Despite there are many attempts to control this insect, most of them depend on conventional pesticides. Thus, the current study aimed to evaluate the efficacy of whole-cell suspension, cell-free supernatant and crude cells of the symbiotic bacteria Photorhabdus sp. and Xenorhabdus sp., as bio-control agents for housefly stages. Materials and Methods: The Photorhabdus sp. and Xenorhabdus sp., were isolated from the entomopathogenic nematodes, Heterorhabditis indica and Steinernema feltiae, respectively.The phenotypic, as well as the enzymatic characterizations of both bacteria, were determined. In addition, histopathological changes of the alimentary canal of M. domestica adults treated with whole-cell suspensions (at 3×10 8 cells mLG 1 ) of both bacteria were carefully examined using transmission electron microscopy. Results: The results showed that both symbiotic bacteria significantly suppressed larvae, pupae and adults of M. domestica, particularly when they were applied as whole-cell suspensions. For example, the highest concentration of whole-cell suspension, cell-free supernatant and crude cells of Photorhabdus sp., induced larval mortalities by 94.7, 64.0 and 45.3%, while those of Xenorhabdus sp., induced larval mortalities by 58.7, 46.7 and 30.7% at 96 hrs, respectively. The results also showed that whole-cell suspensions of both symbiotic bacteria caused severe histopathological changes in the ultrastructure of the treated adultsʼ alimentary canal. Conclusion: Both symbiotic bacteria can be effectively used, particularly the whole-cell suspension, as bio-control agents against the housefly either in the larval or adult stage.

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Section: Discussionmentioning

confidence: 97%

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

El-Rah¹,

Abdelazeem²,

Elbrense³

et al. 2022

Pakistan J. of Biological Sciences

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“…albopictus were reared in insect cages (40 × 40 × 40 cm) in a controlled insectary with a temperature of 30°C, 70 ± 10% relative humidity and a 12 h light: 12 h dark photoperiod. Mosquitoes were fed every 2-3 days on de brinated sheep blood using an arti cial blood feeder (Shah et al 2021). Sugary water (10%) was available at all times.…”

Section: Methodsmentioning

confidence: 99%

Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Touray,

Cimen,

Bode

et al. 2023

Preprint

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Viral diseases like yellow fever, dengue, zika have an alarming impact on public health affecting millions of people around the globe. These diseases can be transmitted by Aedes albopictus – a species originally endemic to South Asia but now widely distributed in several countries outside Asia. Xenorhabdus and Photorhabdus spp., are enteric Gram-negative bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products (NP) with various activities including larvicidal activity. This study demonstrated that supernatants of different Xenorhabdus and Photorhabdus bacteria effectively deterred Ae. albopictus mosquito oviposition in a concentration dependent manner. Xenorhabdus cabanillasii displayed deterrent effects at 50-5% concentrations with oviposition activity index values ranging between -0.87 and -0.35, whereas the index values for X. nematophila were between -0.82 and -0.52 at concentrations of 50-10%. The other bacteria (X. szentirmaii, X. doucetiae, and P. kayaii) were effective at concentrations ≥20%. Using promoter exchange mutants of selected biosynthetic gene clusters in a Δhfq background generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such compounds with deterrence effects could be useful in mosquito control programs as preventing breeding site in specific habitats can greatly influence mosquito species establishment, population densities, and dispersion in conducive areas.

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“…The optimum range for H. bacteriophora is 22-26°C (Doucet et al, 1996) and 25°C for S. feltiae (Belair et al, 2003). The virulent EPN species, identified in the study of Shah et al (2021), were known to work better at higher temperatures, i.e. 20-30 °C (Kim and Alston, 2008;Power et al, 2009).…”

Section: Insect Host Physiologymentioning

confidence: 99%

“…As easily shown in the current literature, many authors (Shapiro-Ilan and Brown, 2013;Shaurub et al, 2014;Lacey et al, 2015;Leonar et al, 2022;Kaliaskaret al, 2022) reported that insect death within 24-72 h post-infection with EPNs, regardless the insect species and the EPN species. On the other hand, several authors (Peschiutta et al, 2014;Cardoso et al, 2015;Liu et al, 2020;Ansari and Hussain, 2020;Shah et al, 2021) described the success of some infected individuals inavoiding the lethal effects of both EPN and its symbiotic bacteria using some innate immune defences. These host individuals developmentally transform into the next stage, but usually unhealthy because certain biological and physiological processes have been adversely affected.…”

Section: Impact Of Epns On Some Biological and Physiological Processe...mentioning

confidence: 99%

Virulence of Entomopathogenic Nematodes and their Symbiotic Bacteria against Insect Pests, with Special Reference to Agrotis ipsilon (Lepidoptera: Noctuidae): A Comprehensive Review.

Ghoneim,

Hassan

2024

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Indiscriminate ingestion of entomopathogenic nematodes and their symbiotic bacteria by Aedes aegypti larvae: a novel strategy to control the vector of Chikungunya, dengue and yellow fever

Cited by 13 publications

References 23 publications

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Virulence of Entomopathogenic Nematodes and their Symbiotic Bacteria against Insect Pests, with Special Reference to Agrotis ipsilon (Lepidoptera: Noctuidae): A Comprehensive Review.

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