Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells

Wan, Lam Yim Murphy; Turner, Paul C.; El-Nezami, Hani

doi:10.1016/j.fct.2013.03.034

Cited by 156 publications

(107 citation statements)

References 82 publications

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“…Deoxynivalenol (DON) and zearalenone (ZEN) are two major mycotoxins produced by Fusarium fungi. Because of the natural co-occurrence of these mycotoxins in food and the globalisation of food markets, there is an increasing concern about health hazards linked to human and animal exposure to mycotoxin mixtures (Ma and Guo 2008;Wan et al 2013). DON and ZEN are among the most frequently detected toxins in Europe.…”

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confidence: 99%

“…Feeding a diet contaminated with 9 mg DON/kg bw to pigs can disturb protein synthesis as well as humoral and cellular immune response, depending on the dose, exposure, and timing of the functional immune assay, and can affect liver and spleen cell structures (Tiemann and Danicke 2007). ZEN has been shown to be hepatotoxic, haematotoxic, immunotoxic, and genotoxic (Wan et al 2013).…”

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confidence: 99%

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The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis

et al. 2015

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ABSTRACT:The examination of caecal contents allows one to investigate the exposure of colon mucosa to dietary toxins and the chemical contaminants present in animal feed. The objective of the present study was to examine the influence of Fusarium mycotoxins (zearalenone, ZEN, and deoxynivalenol, DON), administered separately and as a mixture, on the genotoxicity of caecal water (CW) from gilts fed these mycotoxins. CW genotoxicity was evaluated with the comet assay using the LLC-PK1 porcine epithelial kidney cell line. It was shown that after the first week of the experiment, the presence of DON in animal feed led to increased CW genotoxicity in the proximal colon, while the presence of DON and ZEN + DON had a similar effect in the distal colon (ANOVA, P < 0.05). After the third week of experiment, elevated genotoxicity in the distal colon was observed for all experimental groups of gilts, and it was 62%, 52.4%, and 52.8% higher for ZEN-, DON-, and ZEN + DON-fed animals, respectively, than for control group animals. However, no effect on CW genotoxicity in the proximal colon was seen. After six weeks, the presence of ZEN in the proximal colon increased CW genotoxicity by 103% in comparison with the control group. In the distal colon, after the sixth week, CW genotoxicity in all groups of animals fed with mycotoxin was significantly (by 80% to 116%) higher than in the control group. ZEN and DON administered as a mixture did not lead to an increased genotoxicity compared to either agent administered separately. Generally, the mycotoxins clearly started to increase the genotoxicity of CW from the third week of administration.

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The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis

et al. 2015

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“…Similar results were reported by Alassane-Kpembi et al (1) in a study on pigs. Synergistic interactions can occur at different stages in the same toxicity pathway, or when the presence of one mycotoxin increases absorption or decreases the metabolic degradation of another mycotoxin (27,28,6).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars diagnosed with mixed mycotoxicosis

Zielonka

Jakimiuk

Obremski

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The study evaluated the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars exposed to deoxynivalenol and zearalenone in naturally contaminated feed. The evaluation was performed with the use of the MTT assay and 2 mitogens: lipopolysaccharide (LPS) and concanavalin A. Intensified proliferative processes in T and B lymphocytes were revealed. The mitogenic activity of LPS was more expressed in the lymphocytes of both iliac and jejunal lymph nodes in comparison with the control group. Proliferative activity was higher in iliac lymph nodes than in jejunal lymph nodes. A reverse trend was observed in the percentage of live cells, which was higher in jejunal lymph nodes during the evaluation of lymphocyte proliferation.

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“…W badaniach przeprowadzonych przez Wan i wsp. (38) wykazano zdolność tej mikotoksyny do indukcji ekspresji IL-1a, IL-1b, IL-6, IL-8, TNF-α oraz MCP1. Szczególnie istotną z punktu widzenia indukcji stanów zapalnych wydaje się IL-8.…”

Section: Materiał I Metodyunclassified

Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin

Dąbrowski

Zielonka

Baranowski

et al. 2017

Medycyna Weterynaryjna

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Środki spożywcze i materiały paszowe pochodzenia roślinnego są bardzo często wektorami niskich dawek mikotoksyn fuzaryjnych (28). Ze względu na szeroki zakres ich zastosowań w przemyśle spożywczym i w produkcji pasz, a także wysoki udział produktów zbożowych w diecie człowieka i zwierząt są ważnym elementem odpowiedzialnym za stan zdrowia ludzi oraz wydajność procesu produkcyjnego zwierząt (10). Najczęściej spotykanymi mikotoksynami fuzaryjnymi są zearalenon (ZEN) oraz deoksyniwalenol (DON).Zearalenon i jego metabolity powodują zaburzenia w rozrodzie zwierząt, ponieważ w swej aktywności są podobne do estradiolu (40). α-ZEL jest dominującym metabolitem ZEN występującym w organizmie świń, podczas gdy u innych gatunków zwierząt, jak np. u brojlerów, krów i owiec dominującym produktem metabolizmu ZEN jest β-ZEL o dużo mniejszej aktywności metabolicznej (9). Efekt aktywności ZEN zależy od nasilenia i charakteru procesów metabolicznych mających miejsce w organizmie zwierząt (18). Zależy również od statusu immunologicznego (5, 20) układu rozrodczego, który podlega ciągłym zmianom na skutek zmian hormonalnych wynikających z okresu dojrzewania płciowego, etapu cyklu rozrodczego czy ciąży. Wykazano, że ZEN może być immunotoksyczny w niskich stężeniach (33). W odpowiedzi na obecność mikotoksyny, wrażliwe komórki przechodzą wiele zmian w zakresie istotnych procesów metabolicznych (proliferacji i różnicowania się komórek, apoptozy, syntezy cząsteczek biologicznie aktywnych, itp.) (37). Pomimo że badania nad ZEN zostały ostatnio zintensyfikowane, to istnieje niewiele badań nad jego wpływem na barierę jelitową i potencjalnego związku pomiędzy ekspozycją na ZEN a stanami zapalnymi jelit (13). Dąbrowski M., Zielonka Ł., Baranowski M., Gajęcka M., Rykaczewska A., Gajęcki M. T.Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin Mixed low-dose mycotoxin can also be a cause of temporary immunity decline, as well as a factor responsible for disturbances during the maturation of the immune system.

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Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells

Cited by 156 publications

References 82 publications

The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis

The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosis

Evaluation of the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars diagnosed with mixed mycotoxicosis

Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin

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