Individual and Synergistic Effects of Tea Tree Oil and Neem Extract on Candida albicans Adhesion to Denture Soft Liner

Singhania, Ashika; Sathe, Seema; Ranka, Rajul; Godbole, Surekha

doi:10.7759/cureus.27869

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Many studies have found that TTO can inhibit LPS- [ 14 , 15 ], bacteria- [ 16 ], and Indiana vesiculovirus- [ 17 ] induced host cell inflammation. TTO has diverse components, which mainly include T-4-O, γ-terpinene, α-terpineol, and 1,8-cineole [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Terpinen-4-ol Improves Lipopolysaccharide-Induced Macrophage Inflammation by Regulating Glutamine Metabolism

Liu,

Tang,

Zhang

et al. 2024

Foods

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Terpinen-4-ol (T-4-O) is an important component of tea tree oil and has anti-inflammatory effects. Currently, there are very few studies on the mechanisms by which T-4-O improves lipopolysaccharide (LPS)-induced macrophage inflammation. In this study, LPS-stimulated mouse RAW264.7 macrophages were used as a model to analyze the effects of T-4-O on macrophage inflammatory factors and related metabolic pathways in an inflammatory environment. The results showed that T-4-O significantly decreased the expression levels of inflammatory cytokines induced by LPS. Cellular metabolism results showed that T-4-O significantly decreased the ratio of the extracellular acidification rate and oxygen consumption rate. Non-targeted metabolomics results showed that T-4-O mainly affected glutamine and glutamate metabolism and glycine, serine, and threonine metabolic pathways. qPCR results showed that T-4-O increased the transcript levels of GLS and GDH and promoted glutamine catabolism. Western blotting results showed that T-4-O inhibited the mTOR and IκB, thereby decreasing NF-κB activity. The overall results showed that T-4-O inhibited mTOR phosphorylation to promote glutamine metabolism and increased cell oxidative phosphorylation levels, thereby inhibiting the expression of LPS-induced inflammatory cytokines.

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Section: Discussionmentioning

confidence: 99%

Terpinen-4-ol Improves Lipopolysaccharide-Induced Macrophage Inflammation by Regulating Glutamine Metabolism

Liu,

Tang,

Zhang

et al. 2024

Foods

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“…Its antifungal effect has been demonstrated in several investigations [ 98 , 99 ]. Exposing the DBR samples to neem extract was found effective in minimizing the adherence of C. albicans [ 100 ]. In a recent investigation, neem powder was incorporated into heat and auto-polymerized DBR at 0.5, 1, 1.5, 2, and 2.5 wt.% [ 67 ].…”

Section: Methods Used For Tuning Bioactive and Antimicrobial Dbr Mate...mentioning

confidence: 99%

Prospects on Tuning Bioactive and Antimicrobial Denture Base Resin Materials: A Narrative Review

Al‐Dulaijan

Balhaddad

2022

Polymers

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Denture base resin (DBR) materials are used in dentistry in constructing removable dentures and implant-supported prostheses. A plethora of evidence has demonstrated that DBR materials are associated with a high risk of denture stomatitis, a clinical complication where the soft oral tissues underneath the resin-based material are inflamed. The prevalence of denture stomatitis among denture wearers is high worldwide. Plaque accumulation and the infiltration of oral microbes into DBRs are among the main risk factors for denture stomatitis. The attachment of fungal species, mainly Candida albicans, to DBRs can irritate the underneath soft tissues, leading to the onset of the disease. As a result, several attempts were achieved to functionalize antimicrobial compounds and particles into DBRs to prevent microbial attachment. This review article explored the advanced approaches in designing bioactive and antimicrobial DBR materials. It was reported that using monomer mixtures, quaternary ammonium compounds (QACs), and organic and inorganic particles can suppress the growth of denture stomatitis-related pathogens. This paper also highlighted the importance of characterizing bioactive DBRs to be mechanically and physically sustainable. Future directions may implement a clinical translational model to attempt these materials inside the oral cavity.

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“…Each culture plate was incubated for 72 hours at 37° C andthe number of colony forming unit in each plate of each disinfectant at various dilutions were measured using a digital colony counter (10DCC01 DBK digital colony counter, India).The values were expressed as number of colonies forming units per mL(CFU/mL). 12…”

Section: Adherence Testingmentioning

confidence: 99%

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

MDS¹

2022

Journal of Pharmaceutical Negative Results

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Objective This study aimed to assess the effectivenessof Vaccinium macrocarpon (cranberry)extract on Candida albicans adhesion tocranberry added denture base biomaterial. Method Different proportions of cranberryextract were incorporated into polymer of polymethyl methacrylate (PMMA) self - cure resin material forfabricationfifty of 4 mm x 4mm x 2mm samples. Group I contained 0 wt/wt% cranberry was used as control group. 0.5wt/wt%, 1.0 wt/wt%, 1.5 wt/wt% and 2 wt/wt% was graded as Group II to V in order.Addition of 0.1mL of Candida albicans suspension was done in each well of tissue culture plate and incubated for 48 hours. 2% cranberrywasused as disinfectant solution in four serial dilutions at undiluted, 1:10, 1:100, 1:1000 ratio. Immersion of resin samples in disinfectant solution was done for 10 minutes. A colony forming unit was measuredafter 0.1mL of the solution was plated on as sabouraud dextrose agar (SDA) plate and cultured for 72 hours. Data were subjected to statistical analysis. Results The mean colony forming units (CFU) per mL showed that there were severalconfluent colonieswith all proportions of cranberry extract added poly methyl methacrylate (CA-PMMA) resin samplesin undiluted and 1:10 dilution state with both distilled water and cranberry disinfectants. While immersion indistilled waterat 1 :100 dilution, mean CFU/mL was 2.99 0.29in all groups of CA-PMMA. In 1 :1000 dilution state, the mean SD was 117 0.42.Immersion in cranberry solution at 1:100 and 1:1000 showed 2.65 0.66 and 1.26 0.59 for all groups of CA-PMMA. On comparison between distilled water and cranberry as disinfectants, a statistically significant difference was noted with all groups as p < 0.043. Insignificant variation was observed within the groups with both disinfectants as p > 0.05. Conclusion Adherence of Candida albicans to cranberry added self-cure poly methyl methacrylate biomaterial was evidenced to be less irrespective of extract proportions of 1%, 1.5% and 2% in PMMA.Cranberry’s effectiveness as disinfectant showed lesscandida growth when compared to distilled water immersion. This infers that cranberry has anti-adherence property when used both as a polymer constituent material as well as disinfectant solution.

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Individual and Synergistic Effects of Tea Tree Oil and Neem Extract on Candida albicans Adhesion to Denture Soft Liner

Cited by 4 publications

References 20 publications

Terpinen-4-ol Improves Lipopolysaccharide-Induced Macrophage Inflammation by Regulating Glutamine Metabolism

Terpinen-4-ol Improves Lipopolysaccharide-Induced Macrophage Inflammation by Regulating Glutamine Metabolism

Prospects on Tuning Bioactive and Antimicrobial Denture Base Resin Materials: A Narrative Review

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

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