Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

Filippi, Miriam; Garello, Francesca; Pasquino, Chiara; Arena, Francesca; Giustetto, Pierangela; Antico, Federica; Terreno, Enzo

doi:10.1002/jbio.201800035

Cited by 29 publications

(22 citation statements)

References 46 publications

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“…Direct cell labeling by ICG is useful for monitoring the early stages (1 to 2 weeks) of cell transplantation. [23,24] Transporters that can selectively uptake ICG have great potential to trace the longitudinal fate of implanted cells, including cellular proliferations, and migration. Keeping this in mind, we first identified the fluorescence output, using different concentration of ICG on 293T cells expressing OATP1B3, OATP1B1, and NTCP.…”

Section: Oatp1b3 Mediated Icg Uptake Results In High Fluorescence Outmentioning

confidence: 99%

Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell‐Based Medicine

et al. 2020

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Hepatic transporters facilitate the uptake of xenobiotic compounds and confer immense potential as multimodality reporters, as they can uptake multitude of bioluminescent, fluorescence, near Infra-red (NIR), and magnetic resonance imaging (MRI) compounds. Here, it is aimed to identify the prospects of three human hepatocyte membrane proteins, organic anion transporters (OATP) 1B1, OATP1B3, and sodium-taurocholate cotransporting polypeptide in tracing the transplanted cells dynamics, by the uptake of clinically approved MRI compound, gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (GD-EOB DTPA) or NIR fluorescent dye, Indocyanine Green (ICG). First, OATP1B1 is introduced as new MRI reporter which is the human orthologue of previously established rodent (Oatp1a1) reporter. Comparative sequential assays by assimilating MR image parameters reveal OATP1B3 as superior reporter on T1-weighted images and display highest contrast enhancement reported to-date when using a clinical 3T MR scanner (≈21-fold in vitro; ≈8-fold in vivo). Stably expressing these hepatic transporters have no effect on human adipose derived mesenchymal stem cells (hADSCs) characteristics. However, only OATP1B3 able to trace as few as 2 × 10 5 hADSCs intramuscular xenograft survival time on NIR and 3T MRI. These data suggest that OATP1B3 is relatively a robust Gd-EOB-DTPA/ICG-dependent multimodality reporter in visualizing dynamic processes for cell-based therapies.

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Section: Oatp1b3 Mediated Icg Uptake Results In High Fluorescence Outmentioning

confidence: 99%

Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell‐Based Medicine

et al. 2020

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“…(c) Representative PA monitoring of the cell engraftment over days (excitation wavelength: 810 nm) after spectral unmixing of signal in the right hindlimb. (Reproduced with permission from Filippi et al ()). ICG, indocyanine green; MSC, medicinal signaling cell…”

Section: Pai With Exogenous Contrast Angetsmentioning

confidence: 99%

“…Recently, ICG dye was used to label murine bone marrow-derived mesenchymal stem cells, also known as medicinal signaling cells (MSCs; Filippi et al, 2019). The transplantation of MSCs holds great promise for the treatment of a plethora of human diseases.…”

Section: Small Organic Moleculesmentioning

confidence: 99%

Recent advances in photoacoustic contrast agents for in vivo imaging

Upputuri

Pramanik

2020

WIREs Nanomed Nanobiotechnol

133

119

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Photoacoustic imaging (PAI) is a noninvasive hybrid imaging modality offering rich optical contrast and high depth‐to‐resolution ratio deep‐tissue imaging. Endogenous chromophores present in the body such as hemoglobin, lipid, melanin, and so on provide strong photoacoustic contrast due to their strong light absorption in certain optical window. To enhance the performance of PAI further, researchers have developed several exogenous contrast agents such as metallic nanoparticles, carbon‐based nanomaterials, quantum dots, organic small molecules, semiconducting polymer nanoparticles, and so on. These exogenous contrast agents not only help improving the imaging contrast, but also make targeted molecular imaging possible. In this review article, we first discuss the state‐of‐the‐art PAI techniques with endogenous contrast mechanism. Later, we provide an overview of recent progress in the development of exogenous photoacoustic contrast agents for in vivo imaging applications. Finally, we present the pros/cons of the existing PA contrast agents along with future challenges of contrast agent‐based PAI for biomedical applications. This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices Diagnostic Tools > In Vivo Nanodiagnostics and Imaging

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“…Obvisouly, contrast agents should also possess reactive group for conjugation to targeting moieties. An example is the use of indocyanine green (originally used for fluorescent imaging but it also possesses suitable absorbance properties for PAI 16). This contrast agent was for example targeted to breast cancer using an anti-B7-H3 antibody (a novel breast cancer molecular target) followed by PA imaging 17.…”

Section: Molecular Imaging Componentsmentioning

confidence: 99%

Monoclonal antibody-based molecular imaging strategies and theranostic opportunities

2020

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Molecular imaging modalities hold great potential as less invasive techniques for diagnosis and management of various diseases. Molecular imaging combines imaging agents with targeting moieties to specifically image diseased sites in the body. Monoclonal antibodies (mAbs) have become increasingly popular as novel therapeutics against a variety of diseases due to their specificity, affinity and serum stability. Because of the same properties, mAbs are also exploited in molecular imaging to target imaging agents such as radionuclides to the cell of interest in vivo. Many studies investigated the use of mAb-targeted imaging for a variety of purposes, for instance to monitor disease progression and to predict response to a specific therapeutic agent. Herein, we highlighted the application of mAb-targeted imaging in three different types of pathologies: autoimmune diseases, oncology and cardiovascular diseases. We also described the potential of molecular imaging strategies in theranostics and precision medicine. Due to the nearly infinite repertoire of mAbs, molecular imaging can change the future of modern medicine by revolutionizing diagnostics and response prediction in practically any disease.

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Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

Cited by 29 publications

References 46 publications

Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell‐Based Medicine

Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell‐Based Medicine

Recent advances in photoacoustic contrast agents for in vivo imaging

Monoclonal antibody-based molecular imaging strategies and theranostic opportunities

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