Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub, Cajanus cajan

Datta, C.; Basu, P. S.

doi:10.1016/s0944-5013(00)80047-6

Cited by 164 publications

(88 citation statements)

References 13 publications

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“…Patten and Glick [25,26] have also reported increase in IAA production up to 96 h and attributed to the greater availability of the precursor. Datta and Basu [9] studied the IAA-degrading enzymes responsible for decrease in IAA production after 96-h incubation period. The decrease in IAA production might be due to the production of IAAdegrading enzymes.…”

Section: Iaa Production Profilementioning

confidence: 99%

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra)

2013

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Indole-3-acetic acid (IAA) production was monitored in nine plant growth-promoting rhizobacteria isolated from the rhizospheric soil of alfalfa (Medicago sativa). The isolate producing maximum amount of IAA was identified as Pseudomonas putida UB1 by 16S rRNA partial gene sequencing. Further investigations were carried out for optimal L-tryptophan concentration and other growth parameters for IAA production by P. putida. IAA production increased when the L-tryptophan medium for IAA production was supplemented with sucrose 0.5 %, (NH 4 ) 2 SO 4 10 mg/ml, and tryptophan 0.2 mg/ml at pH 7.5. Maximum IAA production was achieved at 96 h. Production of IAA was confirmed by extraction of crude IAA and subsequent thin-layer chromatography analysis. The specific spot from the extracted IAA was found to correspond with a spot of standard IAA with the same R f value (0.36). The crude IAA was further purified by passing it through a silica gel column. P. putida UB1 and its purified IAA were demonstrated to show stimulatory effect on mustard (Brassica nigra) plants in vitro with their incorporation in MS medium as a novel approach.

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Section: Iaa Production Profilementioning

confidence: 99%

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra)

2013

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“…4a, the production of IAA by RC6b in LB medium supplemented with L-tryptophan (1 mg mL À1 ) exhibited a maximum IAA accumulation (96.5 mg L À1 ) at 72 h of incubation; thereafter, it was decreased and maintained constant for a period of time. This decrease was probably attributed to the release of IAA degrading enzymes such as IAA oxidase and peroxidase (Datta and Basu, 2000). Since root borne nutrients particularly L-tryptophan are considered as an important components for bacterial IAA production as well as for their growth in the rhizosphere, the strain RC6b was further tested for its ability to produce IAA in culture media supplemented with various concentrations of L-tryptophan.…”

Section: Plant Growth Promoting Traits Of P Myrsinacearum Rc6bmentioning

confidence: 99%

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

et al. 2013

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h i g h l i g h t sIsolated Phyllobacterium myrsinacearum RC6b effectively mobilized metals in soils. RC6b inoculation enhanced growth and Cd and Zn uptake of Sedum plumbizincicola. Possible mechanisms are plant beneficial activities and soil metal mobilization. RC6b is a good candidate for microbially assisted phytoremediation. a r t i c l e i n f o t r a c tThe aim of this study was to investigate the effects of metal mobilizing plant-growth beneficial bacterium Phyllobacterium myrsinacearum RC6b on plant growth and Cd, Zn and Pb uptake by Sedum plumbizincicola under laboratory conditions. Among a collection of metal-resistant bacteria, P. myrsinacearum RC6b was specifically chosen as a most favorable metal mobilizer based on its capability of mobilizing high concentrations of Cd, Zn and Pb in soils. P. myrsinacearum RC6b exhibited a high degree of resistance to Cd (350 mg L À1 ), Zn (1000 mg L À1 ) and Pb (1200 mg L À1 ). Furthermore, P. myrsinacearum RC6b showed multiple plant growth beneficial features including the production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, siderophore and solubilization of insoluble phosphate. Inoculation of P. myrsinacearum RC6b significantly increased S. plumbizincicola growth and organ metal concentrations except Pb, which concentration was lower in root and stem of inoculated plants. The results suggest that the metal mobilizing P. myrsinacearum RC6b could be used as an effective inoculant for the improvement of phytoremediation in multi-metal polluted soils.

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“…The IAA production by the B. cereus isolates started after the inoculation and maximum IAA production was obtained in the stationary growth phase at 72h followed by a decrease. As stated previously, the decrease in IAA production after 72 h might be due to the synthesis of IAA degrading enzymes such as IAA oxidase and peroxidase [17,18]. Unyayar et al [19] reported that the amount of these enzymes were high in the stationary phase.…”

Section: Screening Of B Cereus Isolates For Iaa Productionmentioning

confidence: 78%

“…This may be due to providing energy for cells and improved [8,22]. Many researchers reported that glucose addition to the culture medium increased IAA synthesis [17,23]. An asterisk (*) denotes a value significantly greater than the other value (P<0.05).…”

Section: Effect Of Glucose On Iaa Productionmentioning

confidence: 99%

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Özdal¹,

Ozdal²,

Sezen³

et al. 2016

CSJ

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Abstract. Four different strains of Bacillus cereus producing indole-3-acetic acid (IAA) were isolated from various rhizospheric soils and characterized biochemically. The isolates were screened for (IAA) production and BC-On strain was found to be the best IAA producer with 46.25 mg/l. Maximum IAA production was obtained in the stationary growth phase at 72h. Significant correlation was also observed between IAA production and growth of the B. cereus strains. Among the isolates, BC-On strain was further used for immobilization studies. Maximum IAA production was obtained at initial pH of 8.0 and temperature of 35 °C after 18 h of fermentation. The immobilized cells could be effectively reused thirteen times and the IAA concentration of 300 mg/l was determined during this period. Results showed that immobilized cells can be used in the continuous process for the production of IAA. The productivity obtained by immobilization was higher than the one obtained by submerged cultivation and immobilization reduced the fermentation time.Keywords: Indole-3-acetic acid, Rhizobacteria, Bacillus cereus, Immobilization Immobilize Bacillus cereus Hücreleri Tarafından İndol-3-Asetik Asitin BiyosenteziÖzet. İndol-3-asetik asit (İAA) üreten dört farklı Bacillus cereus straini çeşitli rizosfer topraklarından izole edildi ve biyokimyasal olarak karakterize edilmiştir. İzolatlar İAA üretimi için taranmış ve BC-On straini 46.25 mg/l ile en iyi İAA üreticisi olarak bulunmuştur. En fazla IAA üretimi 72. saatte durağan büyüme fazında elde edilmiştir. B. cereus strainlerinin IAA üretimi ve büyüme arasında önemli bir ilişki gözlenmiştir. İzolatlar arasında, BC-On straini immobilizasyon çalışmalarında kullanılmıştır. En fazla IAA üretimi 18 saatlik fermentasyon sonrasında başlangıç pH'sı 8.0 ve 35 °C elde edilmiştir. İmmobilize edilmiş hücreler etkili olarak on üç kez kullanılabilmiş ve bu süre boyunca 300 mg/l İAA konsantrasyonu tespit edilmiştir. Sonuçlar immobilize hücrelerin IAA üretimi için sürekli bir işlemde kullanılabilir olduğunu göstermiştir. İmmobilizasyon ile elde edilen verimlilik, derin fermentasyon ile elde edilenden daha yüksek olduğu bulunmuş ve immobilizasyon fermentasyon süresini kısaltmıştır.

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Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub, Cajanus cajan

Cited by 164 publications

References 13 publications

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra)

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra)

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

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