Indole alkaloids from Ervatamia chinensis

Guo, Ling‐Li; He, Hongping; Di, Ying‐Tong; Li, Shi-Fei; Cheng, Yuanyuan; Yang, Wei; Li, Yan; Yu, Jian; Zhang, Yu; Hao, Xiao‐Jiang

doi:10.1016/j.phytochem.2011.11.002

Cited by 69 publications

(49 citation statements)

References 25 publications

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“…[1827] Also, the better extraction of alkaloids such as jubanin A, amphibian H and spinanine A may explain the better effect of chloroform and chloroform-methanol extracts of Z. spina-christi on both tested cell lines. [28] It hase been shown that some alkaloids possess good cytotoxic activity against different cell lines[2930] The cytotoxic effect of butanolic extract could be explained by the presence of saponin such as betulic acid in this extract. [31]…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells

2014

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Background:It has been shown that plants from the family Rhamnaceae possess anticancer activity. In this study, we sought to determine if Ziziphus spina-christi, a species from this family, has cytotoxic effect on cancer cell lines.Materials and Methods:Using maceration method, different extracts of leaves of Z. spina-christi were prepared. Hexane, chloroform, chloroform-methanol (9:1), methanol-water (7:1) methanol, butanol and water were used for extraction, after preliminary phytochemical analyses were done. The cytotoxic activity of the extracts against Hela and MDA-MB-468 tumor cells was evaluated by MTT assay. Briefly, cells were seeded in microplates and different concentrations of extracts were added. After incubation of cells for 72 h, their viability was evaluated by addition of tetrazolium salt solution. After 3 h medium was aspirated, dimethyl sulfoxide was added and absorbance was determined at 540 nm with an ELISA plate reader. Extracts were considered cytotoxic when more than 50% reduction on cell survival was observed.Results:Hexane, chloroform, chloroform-methanol, butanol, methanol-water and aqueous extracts of Z. spina-christi significantly and concentration-dependently reduced viability of Hela and MAD-MB-468 cells. In the both cell lines, chloroform-methanol extract of Z. spina-christi was more potent than the other extracts.Results:From the finding of this study it can be concluded that Z. spina-christi is a good candidate for further study for new cytotoxic agents.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells

2014

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“…ibogaine (6), ibogaline (7), desethyl-voacangine (8), voachalotine (9), and affinisine (10) from the chloroform extract of stalk of T. australis (Müell. Arg) Miers by gas chromatography-mass spectrometry (GC-MS) (Scheme 3).…”

Section: Bioactive Compounds From the Genus Tabernaemontanamentioning

confidence: 99%

“…Many of plants in the Tabernaemontana species are used in traditional medicine and for other purposes for the treatment of sore throat, hypertension, and abdominal pain [4][5][6]. Plants of Tabernaemontana genus are a prolific source of the monoterpene indole alkaloids and they have been shown to produce many skeletal types such as secotabersonine alkaloids, bisvobtusine-type alkaloids, and bis-vobasinyl-ibogan indole alkaloids [7][8][9]. These alkaloids are a diverse class of natural products, comprising over 2000 members and possess a range of chemical structures and a wealth of biological activities such as anticancer, antimalarial, and antiarrhythmic agents [10].…”

Section: Introductionmentioning

confidence: 99%

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

Athipornchai

2018

Asian J Pharm Clin Res

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Plants in the genus Tabernaemontana have been using in Thai and Chinese traditional medicine for the treatment several diseases. The great majority constituents of Tabernaemontana species have already been subjected to isolation and identification of monoterpene indole alkaloids present in their several parts. Many of monoterpene indole alkaloids exhibited a wide array of several activities. The biogenesis, classification, and biological activities of these alkaloids which found in Tabernaemontana plants were discussed in this review and its brings the research up-to-date on the bioactive compounds produced by Tabernaemontana species, directly or indirectly related to human health.

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“…The SCC2095 (oral squamous cell carcinoma cell line) cell line was used in the anti-proliferation assay according to the previously described procedure (Guo et al, 2012). The cells were cultured in MDMEM medium (Hyclone, USA), supplemented with 10% of fetal bovine serum in 5% CO 2 at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Developing an activity and absorption-based quality control platform for Chinese traditional medicine: Application to Zeng-Sheng-Ping(Antitumor B)

Yin

Yang

et al. 2015

Journal of Ethnopharmacology

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Ethnopharmacological relevance Zeng-Sheng-Ping (ZSP) is a marketed Chinese traditional medicine used for cancer prevention. Aim of the study Currently, for the quality control of Chinese traditional medicines, marker compounds are not selected based on bioactivities and pharmaceutical behaviors in most of the cases. Therefore, even if the “quality” of the medicine is controlled, the pharmacological effect could still be inconsistent. The aim of this study is to establish an activity and absorption-based platform to select marker compound(s) for the quality control of Chinese traditional medicines. Materials and methods We used ZSP as a reference Chinese traditional medicine to establish the platform. Activity guided fractionation approach was used to purify the major components from ZSP. NMR and MS spectra were used to elucidate the structure of the isolated compounds. MTT assay against oral carcinoma cell line (SCC2095) was performed to evaluate the activities. UPLC-MS/MS was used to quantify the pure compounds in ZSP and the active fraction. The permeabilities of the identified compounds were evaluated in the Caco-2 cell culture model. The intracellular accumulation of the isolated compounds was evaluated in the SCC2095 cells. Results The major compounds were identified from ZSP. The contents, anti-proliferation activities, permeabilities, and intracellular accumulations of these compounds were also evaluated. The structure of these purified compounds were identified by comparing the NMR and MS data with those of references as rutaevine (1), limonin (2) , evodol (3), obacunone (4), fraxinellone (5), dictamnine (6), maackiain (7), trifolirhizin (8), and matrine (9). The IC50 of compounds 5, 6, and 7 against SCC2095 cells were significantly lower than that of ZSP. The uptake permeability of compounds 5, 6, and 7 were 2.58 ± 0. 3 × 10−5, 4.33 ± 0.5 × 10−5, and 4.27 ± 0.8 × 10−5 respectively in the Caco-2 cell culture model. The intracellular concentrations of these compounds showed that compounds 5, 6, and 7 were significantly accumulated inside the cells. Conclusion Based on the activity against oral carcinoma cell line as well as the absorption permeability, compound 5, 6, and 7 are selected as quality control markers for ZSP. A activity and absorption-based platform was established and successfully used for the quality control of ZSP.

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Indole alkaloids from Ervatamia chinensis

Cited by 69 publications

References 25 publications

Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells

Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

Developing an activity and absorption-based quality control platform for Chinese traditional medicine: Application to Zeng-Sheng-Ping(Antitumor B)

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