Indoleamines and calcium channels influence morphogenesis in in vitro cultures of<i>Mimosa pudica L.</i>

Ramakrishna, A; Giridhar, P.; Ravishankar, G. A.

doi:10.4161/psb.4.12.10101

Cited by 49 publications

(16 citation statements)

References 26 publications

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“…In brassica juncea plant, exogenously applied melatonin enhanced the IAA level, which further resulted in better root activity [ 67 ]. Whereas it influenced the root organogenesis positively in Mimosa pudica L. [ 68 ]. Therefore, it is assumed that melatonin influenced signal transduction and also had a role in regulating plant physiological and biological processes.…”

Section: Role Of Melatonin In Regulating Plant Growth and Physiolomentioning

confidence: 99%

Melatonin and Its Effects on Plant Systems

et al. 2018

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Melatonin (N-acetyl-5-methoxytryptamine) is a nontoxic biological molecule produced in a pineal gland of animals and different tissues of plants. It is an important secondary messenger molecule, playing a vital role in coping with various abiotic and biotic stresses. Melatonin serves as an antioxidant in postharvest technology and enhances the postharvest life of fruits and vegetables. The application of exogenous melatonin alleviated reactive oxygen species and cell damage induced by abiotic and biotic stresses by means of repairing mitochondria. Additionally, the regulation of stress-specific genes and the activation of pathogenesis-related protein and antioxidant enzymes genes under biotic and abiotic stress makes it a more versatile molecule. Besides that, the crosstalk with other phytohormones makes inroads to utilize melatonin against non-testified stress conditions, such as viruses and nematodes. Furthermore, different strategies have been discussed to induce endogenous melatonin activity in order to sustain a plant system. Our review highlighted the diverse roles of melatonin in a plant system, which could be useful in enhancing the environmental friendly crop production and ensure food safety.

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Section: Role Of Melatonin In Regulating Plant Growth and Physiolomentioning

confidence: 99%

Melatonin and Its Effects on Plant Systems

et al. 2018

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“…As both the receptors and mechanisms underlying the functions of melatonin in plants while an active area of research are still poorly understood, in vitro systems offer a valuable platform for their investigation (Lee and Back, 2016; Sanchez-Barcelo et al, 2016; Shi et al, 2016; Wei et al, 2016). One such important strategy, which has helped to confirm for example the importance of calcium signaling in melatonin responses in several species, is the inclusion of inhibitors in plant medium (Murch et al, 2001; Jones et al, 2007; Ramakrishna et al, 2009, 2011). The in vitro culture system also offers a unique opportunity compared to greenhouse and field trials, in that treatment conditions can be closely monitored and allow for treatment of many plants, and all cultures are maintained in aseptic conditions.…”

Section: Discussionmentioning

confidence: 99%

Melatonin in Plants and Plant Culture Systems: Variability, Stability and Efficient Quantification

et al. 2016

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Despite growing evidence of the importance of melatonin and serotonin in the plant life, there is still much debate over the stability of melatonin, with extraction and analysis methods varying greatly from lab to lab with respect to time, temperature, light levels, extraction solvents, and mechanical disruption. The variability in methodology has created conflicting results that confound the comparison of studies to determine the role of melatonin in plant physiology. We here describe a fully validated method for the quantification of melatonin, serotonin and their biosynthetic precursors: tryptophan, tryptamine and N-acetylserotonin by liquid chromatography single quadrupole mass spectrometry (LC-MS) in diverse plant species and tissues. This method can be performed on a simple and inexpensive platform, and is both rapid and simple to implement. The method has excellent reproducibility and acceptable sensitivity with percent relative standard deviation (%RSD) in all matrices between 1 and 10% and recovery values of 82–113% for all analytes. Instrument detection limits were 24.4 ng/mL, 6.10 ng/mL, 1.52 ng/mL, 6.10 ng/mL, and 95.3 pg/mL, for serotonin, tryptophan, tryptamine, N-acetylserotonin and melatonin respectively. Method detection limits were 1.62 μg/g, 0.407 μg/g, 0.101 μg/g, 0.407 μg/g, and 6.17 ng/g respectively. The optimized method was then utilized to examine the issue of variable stability of melatonin in plant tissue culture systems. Media composition (Murashige and Skoog, Driver and Kuniyuki walnut or Lloyd and McCown's woody plant medium) and light (16 h photoperiod or dark) were found to have no effect on melatonin or serotonin content. A Youden trial suggested temperature as a major factor leading to degradation of melatonin. Both melatonin and serotonin appeared to be stable across the first 10 days in media, melatonin losses reached a mean minimum degradation at 28 days of approximately 90%; serotonin reached a mean minimum value of approximately 60% at 28 days. These results suggest that melatonin and serotonin show considerable stability in plant systems and these indoleamines and related compounds can be used for investigations that span over 3 weeks.

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“…The importance of mediation of H 2 O 2 levels in root growth and morphogenesis as a function of melatonin is highlighted with H 2 O 2 mentioned in ∼22% of all papers on the topics of "root" and "melatonin." The importance of downstream signaling cascades has also been investigated, with calcium having been shown to play an essential role in mediating root growth (n = 7) including in Mimosa pudica L. (Ramakrishna et al, 2009). The use of transcriptomics approaches will likely continue to elucidate significant cross talk between auxin-dependent and auxin-independent mechanisms of melatonin action in plants which have been utilized extensively (n = 42) to study root physiology in response to melatonin treatment.…”

Section: Major Research Theme -Roots Are Importantmentioning

confidence: 99%

“…A later report investigated thidiazuroninduced morphogenesis in Echinaceae purpurea L. suggested interaction between melatonin and calcium, as treatment with calcium transport inhibitors lead to an increase in melatonin content (Jones et al, 2007). The first direct report of dependence of melatonin action in plants was reported in 2009, where melatonin induced shoot morphogenesis in Mimosa pudica L. (Ramakrishna et al, 2009). These effects were found to be inhibited by treatment with the calcium channel blocker verapamil, or addition of calcium chelators to the medium (Ramakrishna et al, 2009).…”

Section: Major Research Theme -Phytohormone and Plant Signalingmentioning

confidence: 99%

A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

Murch

Erland

2021

Front. Plant Sci.

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Melatonin (N-acetyl-5-methoxy-tryptamine) is a mammalian neurohormone, antioxidant and signaling molecule that was first discovered in plants in 1995. The first studies investigated plant melatonin from a human perspective quantifying melatonin in foods and medicinal plants and questioning whether its presence could explain the activity of some plants as medicines. Starting with these first handful of studies in the late 1990s, plant melatonin research has blossomed into a vibrant and active area of investigation and melatonin has been found to play critical roles in mediating plant responses and development at every stage of the plant life cycle from pollen and embryo development through seed germination, vegetative growth and stress response. Here we have utilized a systematic approach in accordance with the preferred reporting items for systematic reviews and meta-analyses (PRISMA) protocols to reduce bias in our assessment of the literature and provide an overview of the current state of melatonin research in plants, covering 1995–2021. This review provides an overview of the biosynthesis and metabolism of melatonin as well as identifying key themes including: abiotic stress responses, root development, light responses, interkingdom communication, phytohormone and plant signaling. Additionally, potential biases in the literature are investigated and a birefringence in the literature between researchers from plant and medical based which has helped to shape the current state of melatonin research. Several exciting new opportunities for future areas of melatonin research are also identified including investigation of non-crop and non-medicinal species as well as characterization of melatonin signaling networks in plants.

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Indoleamines and calcium channels influence morphogenesis in in vitro cultures ofMimosa pudica L.

Cited by 49 publications

References 26 publications

Melatonin and Its Effects on Plant Systems

Melatonin and Its Effects on Plant Systems

Melatonin in Plants and Plant Culture Systems: Variability, Stability and Efficient Quantification

A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

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