Indomethacin electrospun nanofibers for colonic drug delivery: In vitro dissolution studies

Abstract

Generally, although the conventional drug delivery systems, such as using only pH-dependent polymers or time-dependent release systems are popular, the individuals' variations of physiological conditions usually lead to premature or imperfect drug release from each of these systems. Therefore, a combination of pH- and time-dependent polymers could be more reliable for delivering drugs to the lower GI tract such as colon. To this end, electrospinning method was used as a fabrication approach for preparing electrospun nanofibers of indomethacin aimed for colon delivery. Formulations were prepared based on a 3 full factorial design. Independent variables were the drug:polymer ratio (with the levels of 3:5, 4.5:5 and 6:5w/w) and Eudragit S:Eudragit RS w/w ratio (20:80, 60:40 and 100:0). The evaluated responses were drug release at pH 1.2, 6.4, 6.8 and 7.4. Combinations of Eudragit S (ES), Eudragit RS (ERS) and drug based on factorial design were loaded in 10ml syringes. Electrospinning method was used to prepare electrospun nanofibers from electrospinning solutions. Conductivity and the viscosity of the solutions were analyzed prior to electrospinning. After collection, the nanofibers were evaluated in terms of morphology and drug release. It was shown that the ratio of drug:polymer and polymer:polymer were pivotal factors to control the drug release from nanofibers. A formulation containing Eudragit S:Eudragit RS (60:40) and drug:polymer ratio of 3:5 exhibited the most appropriate drug release as a colon delivery system with a minor release at pH 1.2, 6.4 and 6.8 and major release at pH 7.4. Nanofibers resulted from this formulation were also more uniform and contained fewer amounts of beads. It was demonstrated that the electrospinning could be regarded as a modern approach for the preparation of colon drug delivery systems leading to marketable products.

Characterization and in vitro evaluation of electrospun aligned‐fiber membranes of poly(L‐co‐D,L‐lactic acid)

“…Random and aligned fibers produced by electrospinning have been studied for different applications in the biomedical area, such as bone tissue engineering, vascular grafts, drug delivery, wound dressing, nerve regeneration, and so on. It is important to highlight that orientated fibers can influence cell adhesion, cell growth and modulate elongated cellular patterns that are typical of the morphology found in native tissues .…”

Preparation of Poly(lactic‐co‐glycolic acid)‐Based Composite Microfibers for Postoperative Treatment of Tumor in NIR I and NIR II Biowindows

“…Electrospinning has been reported as a facile and powerful technology to generate synthetic or natural polymer‐based fibrous materials . Electrospun nano/microfibers are featured with tunable fiber diameter, high porosity, ease of surface functionalization, and flexible mechanical durability, which render electrospun nano/microfibers a promising application future in energy storage, environmental remediation, catalysis, sensors, and drug delivery systems . As one of the U.S. Food and Drug Administration (FDA)‐approved biocompatible and biodegradable polymers, poly(lactic‐ co ‐glycolic acid) (PLGA) has been frequently employed in electrospinning to form nano/microfibers for biomedical applications such as antitumor and antibacterial .…”

“…[25,26] Electrospun nano/microfibers are featured with tunable fiber diameter, high porosity, ease of surface functionalization, and flexible mechanical durability, [26,27] which render electrospun nano/microfibers a promising application future in energy storage, [28] environmental remediation, [28] catalysis, [29] sensors, [30] and drug delivery systems. [31,32] As one of the U.S. Food and Drug Administration (FDA)-approved biocompatible and biodegradable polymers, poly(lactic-co-glycolic acid) (PLGA) has been frequently employed in electrospinning to form nano/microfibers for biomedical applications such as antitumor [33] and antibacterial. [34] However, to the best of our knowledge, the in vivo tumor therapy of PLGA nano/microfibers remains a challenge.In a previous study, we constructed a photothermal agentand chemotherapeutic drug-containing PLGA/MoS 2 /DOX composite implant based on the "liquid-solid" phase transformation of PLGA/MoS 2 /DOX oleosol.…”

Terapia Fotodinâmica Em Eletrofiação: Revisão De Técnicas E Aplicações

“…[11][12][13] As aplicações para as nanofibras se estendem em vários ramos distintos, tais como medicina, engenharia de tecidos, filtração, energia e eletrônica, sensores e roupas protetivas. 14 Sua utilização na medicina já relatada abrange a entrega de fármacos, 15 curativos 16 e dispositivos hemostáticos. 17 Quando citada a engenharia de tecidos, as mesmas podem ser como vasos sanguíneos 18 e regeneração de nervos, 19 scaffold 3D para a regeneração de tecidos, 18 membrana porosa para a regeneração da pele.…”