Induced apoptosis in melanocytes cancer cell and oxidation in biomolecules through deuterium oxide generated from atmospheric pressure non-thermal plasma jet

Abstract

Recently, atmospheric-pressure non-thermal plasma-jets (APPJ) are being for the cancer treatment. However, APPJ still has drawbacks such as efficiency and rise in temperature after treatment. So, in this work, a synergetic agent D2O vapour is attached to APPJ which not only increase the efficiency of plasma source against cancer treatment, but also controlled the temperature during the treatment. OD generated by the combination of D2O + N2 plasma helped in enhancing the efficiency of APPJ. We observed OD induced apoptosis on melanocytes G361 cancer cells through DNA damage signalling cascade. Additionally, we observed that plasma induces ROS, which activated MAPK p38 and inhibits p42/p44 MAPK, leading to cancer cell death. We have also studied DNA oxidation by extracting DNA from treated cancer cell and then analysed the effects of OD/OH/D2O2/H2O2 on protein modification and oxidation. Additionally, we attempted molecular docking approaches to check the action of D2O2 on the apoptosis related genes. Further, we confirmed the formation of OD/OH simultaneously in the solution using optical emission spectroscopy. Moreover, the simultaneous generation of D2O2/H2O2 was detected by the use of confocal Raman spectroscopy and density measurements.

Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet

“…The experimental data obtained by our group and by other groups on the RONS demonstrated that H 2 O 2 is one of the main and stable species that can result in structural changes of biomolecules due to its oxidation process and it can enhance anticancer therapy9101235 Therefore, for better understanding, we verified the molecular dynamic simulation using 10% H 2 O 2 with Hb and Mb. We measured the RMSD values, which indicate that the presence of H 2 O 2 does not affect the stability of Hb and Mb structures in MD simulations.…”

“…CAP is therefore extremely appropriate for the sterilization of immobilized microorganisms on a material surface under dry and wet conditions, as well as for a wide variety of other practical applications5678. Additionally, CAP shows promising anticancer activities through apoptosis and cell cycle arrest, which have been shown to lead to the destruction of tumor cells in both in vitro and in vivo experiments910111213. Both anticancer activity and the inactivation of microbes by CAP are due to the generation of plasma agents such as photons, electric field, charged particles, and radicals, all of which have different effects on all biomolecular systems.…”

New arylated benzo[h]quinolines induce anti-cancer activity by oxidative stress-mediated DNA damage

“…All the cells were maintained in RPMI and DMEM supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 1% L-glutamine (300 μg/mL), 1% penicillin (100 IU/ml) and streptomycin (100 mg/ml) (Hyclone, USA)323647. Cultured cells were grown at 37 °C, 95% relative humidity and 5% CO 2 and passaged twice a week.…”

“…Cell viability was measured by the MTT assay343648. Briefly, 2 × 10 5 cells/well were seeded in 96-well plates and incubated for 12 h at 37 °C in a 5% CO2 atmosphere to allow cell adhesion.…”

“…A 100 μL solution of compound ( 3e, 3f, 3h and 3j ) was added respectively to a 100-μL of fresh medium in wells to give final concentrations of 5–0.312 μM/mL after serial dilution. A negative control (without drug), a positive control with anticancer drug doxorubicin was included in each assay3436.…”