Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet

Abstract

Over the last few years, the variation in liquid chemistry due to the development of radicals generated by cold atmospheric plasma (CAP) has played an important role in plasma medicine. CAP direct treatment or CAP activated media treatment in cancer cells shows promising anticancer activity for both in vivo and in vitro studies. However, the anticancer activity or antimicrobial activity varies between plasma devices due to the different abilities among plasma devices to generate the reactive oxygen and nitrogen species (RONS) at different ratios and in different concentrations. While the generation of RONS depends on many factors, the feeding gas plays the most important role among the factors. Hence, in this study we used different compositions of feeding gas while fixing all other plasma characteristics. We used Ar, Ar-O2 (at different ratios), and Ar-N2 (at different ratios) as the working gases for CAP and investigated the structural changes in proteins (Hemoglobin (Hb) and Myoglobin (Mb)). We then analyzed the influence of RONS generated in liquid on the conformations of proteins. Additionally, to determine the influence of H2O2 on the Hb and Mb structures, we used molecular dynamic simulation.

The protective action of osmolytes on the deleterious effects of gamma rays and atmospheric pressure plasma on protein conformational changes

“…All chemicals and reagents were used without further purification. The concentrations of OH, NO, H 2 O 2 , NO 2 − , and NO 3 − were measured by methods provided in our previous work 69 – 72 . The NO is detected using 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM), the excitation/emission maxima is 495/515 nm for the DAF-FM-T (product of the reaction between the NO and DAF-FM).…”

“…Each sample spectrum was obtained by subtracting the appropriate blank media without myoglobin from the experimental protein spectrum. The percentages of secondary structures were then calculated using Yang’s method 69 .…”

“…Crystal structures of the human myoglobin were obtained from the (RCSB) protein data bank website ( http://www.rcsb.org ; the PDB IDs were 1MBN) 69 . These structures were treated with the Protein Preparation Wizard of the Schrödinger suite for molecular dynamics (MD) simulations.…”

Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas

“…There have been recent studies on the precise molecular mechanism governing the interactions between plasmas with bio-macromolecules and plasmas have been shown to cause loss of biological functions and structural changes in biomolecules in vitro. [27][28][29][30][31][32][33][34][35][36] P. Attri et al studied the structural changes in hemoglobin (Hb) in the presence of RS generated by the dielectric barrier discharge (DBD) plasma in the presence of various gases such as O 2 , N 2 , Ar, He, NO (10%) + N 2 and air. 33 S. Choi et al used lysozyme as a model enzyme and veried the plasma-induced structural changes by circular dichroism (CD), uorescence, and X-ray crystallography.…”

Inactivation of myeloma cancer cells by helium and argon plasma jets: The effect comparison and the key reactive species

“…8 While modulating the cancer cells inactivation effect, some studies focus on the external parameters of the plasma source, such as the applied voltage, the discharge power, the waveform and frequency of the voltage, and the electrode structure. [9][10][11] Some try to change the concentration of downstream reactive species. 12,13 However, regardless of which parameters are adjusted in the upstream gas region, the effect of the application is achieved by regulating the type and concentration of reactive species in the downstream liquid region where the tissues and cells exist.…”