Induced biosynthesis of resveratrol and the prenylated stilbenoids arachidin-1 and arachidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage

Condori, Jose; Sivakumar, G.; Hubstenberger, John F.; Dolan, Maureen C.; Соболев, В. С.; Medina-Bolívar, Fabricio

doi:10.1016/j.plaphy.2010.01.008

Cited by 84 publications

(88 citation statements)

References 39 publications

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“…Several attempts have also been made to enhance hairy root growth and their production of important bioactive compounds (Yu et al, 1996;Yu et al, 2006;Satdive et al, 2007;Shinde et al, 2009). Optimizing the composition of inorganic nutrients of the media for hairy root cultures is essential to gain high production of secondary metabolites (Condori et al, 2010;Shinde et al, 2010). It has been reported that concentration of nitrogen and appropriate ratio of nitrogen sources in the culture medium greatly affected the growth and production of secondary metabolites in hairy root cultures (Oksman-Caldentey et al, 1994;Yu et al, 1996;Lourenco et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Parizi

Farsi

Nematzadeh³

et al. 2015

AAS

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Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH 4 + to NO 3 -ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B 5 and ½ B 5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM) of NH 4 + to NO 3 -displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots. Key words: Valeriana officinalis, hairy root, medium composition IZVLEČEK VPLIV RAZLIČNIH GOJITVENIH GOJIŠČ NA RAST TRANSFORMIRANIH KORENIN ZDRAVILNE ŠPAJKE (Valeriana officinalis L.)Transformirana kultura korenin zdravilne špajke je bila vzpostavljena z bakterijsko okužbo Agrobacterium rhizogenes, sev ATCC 15834. Preučevana so bila različna gojišča MS, B5 (1,0X in 0,5X koncentracija), N6 in modificiran MS brez fitohormonov. Dodatno so bila v MS gojišču preučevana različna razmerja med NH 4 + in NO 3 -. Učinki teh tretmajev na rast transformiranih korenin so bili ovrednoteni po 21 dneh. B 5 in ½ B 5 sta bili najboljši osnovni gojišči za rast transformiranih korenin. MS gojišče dopolnjeno z dušikovimi spojinami v razmerju 20:20 (mM) NH 4 + : NO 3 -je vplivalo na največjo rast in biomaso korenin. Raziskava je pokazala, da imata sestava rastnega gojišča in različna razmerja dušikovih spojin značilen vpliv na rast transformiranih korenin zdravilne špajke.

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Section: Introductionmentioning

confidence: 99%

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Parizi

Farsi

Nematzadeh³

et al. 2015

AAS

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“…The extraction was carried out using the agitation conditions usually applied during the normal culture process of hairy roots, and other stronger agitation methods were tested to explore the possibility of minimizing the extraction time. The shaking method using rotary shakers is usually applied during the growth of hairy root cultures [5,15], therefore shaking for 24 h using a rotary shaker at a speed of 100 rpm at 25°C in darkness was selected as the extraction method for subsequent studies.…”

Section: Extraction Time and Agitation Methodsmentioning

confidence: 99%

“…However, the organic solvent/aqueous medium ratio is usually 1:1 (v/v) thus large amounts of solvent are needed [3,5,6,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Development of a liquid-liquid extraction method of resveratrol from cell culture media using solubility parameters

Balkhi

Mohammad

Tisserant

et al. 2016

Separation and Purification Technology

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The extraction of bioactive compounds, produced by plant cell cultures, directly from their culture medium, which contains other by-products, is a great challenge. Resveratrol extraction from its grapevine cell cultures is considered here as an example to optimize improve the extraction processes from plant cell cultures using solubility parameters.Successive liquid-liquid extraction (LLE) processes were exploited to extract resveratrol from the culture medium with an extraction ratio approaching 100%, high selectivity and minimum amounts of solvents. The calculations of partition coefficients as a function of solubility parameters demonstrated that benzyl benzoate is the most suitable intermediate solvent to extract resveratrol from its aqueous medium. The calculations also illustrated the high ability of methanol and ethanol to extract resveratrol from benzyl benzoate. The physicochemical properties of benzyl benzoate and processing conditions were exploited to separate it from aqueous media and organic solvents. The agitation method, component ratios and extraction time were studied to maximize the extraction yield. Under the best conditions, the recovery of resveratrol from different culture media approached ~100% with a selectivity of ~92%.2 Ultimately, the improved extraction processes of resveratrol are markedly efficient, selective, rapid and economical.

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“…Hairy root cultures of peanut 'Hull' line 3 established by the Medina-Bolivar laboratory (Condori et al, 2010) were maintained in 50 mL of modified Murashige and Skoog medium (Condori et al, 2010) with 3% Suc in 250-mL flasks. In order to induce the synthesis and secretion of stilbenoids, the spent medium of 9-d-old hairy root cultures was discarded and replaced with 50 mL of fresh Murashige and Skoog medium containing 3% Suc with 100 mM MeJA and 9 g L 21 CD (Cavasol) as elicitors and incubated in the dark at 28°C for an additional 72 h as described previously (Yang et al, 2015a).…”

Section: Analyses Of Stilbenoids From Peanut Hairy Root Culturesmentioning

confidence: 99%

“…In order to elucidate the biosynthesis of peanut prenylated stilbenoids, we established hairy root cultures of peanut (Condori et al, 2010) and recently demonstrated that a sustainable production of the prenylated stilbenoids arachidin-1 and arachidin-3 can be achieved upon cotreatment of these cultures with methyl jasmonate (MeJA) and cyclodextrin (CD; Yang et al, 2015a). In this study, we took advantage of this bioproduction system and identified arachidin-2 and a new prenylated stilbenoid, named arachidin-5, as the prenylated products of the hairy root microsomal fraction using resveratrol and piceatannol as substrates, respectively.…”

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confidence: 99%

A Stilbenoid-Specific Prenyltransferase Utilizes Dimethylallyl Pyrophosphate from the Plastidic Terpenoid Pathway

et al. 2016

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Prenylated stilbenoids synthesized in some legumes exhibit plant pathogen defense properties and pharmacological activities with potential benefits to human health. Despite their importance, the biosynthetic pathways of these compounds remain to be elucidated. Peanut (Arachis hypogaea) hairy root cultures produce a diverse array of prenylated stilbenoids upon treatment with elicitors. Using metabolic inhibitors of the plastidic and cytosolic isoprenoid biosynthetic pathways, we demonstrated that the prenyl moiety on the prenylated stilbenoids derives from a plastidic pathway. We further characterized, to our knowledge for the first time, a membrane-bound stilbenoid-specific prenyltransferase activity from the microsomal fraction of peanut hairy roots. This microsomal fraction-derived resveratrol 4-dimethylallyl transferase utilizes 3,3-dimethylallyl pyrophosphate as a prenyl donor and prenylates resveratrol to form arachidin-2. It also prenylates pinosylvin to chiricanine A and piceatannol to arachidin-5, a prenylated stilbenoid identified, to our knowledge, for the first time in this study. This prenyltransferase exhibits strict substrate specificity for stilbenoids and does not prenylate flavanone, flavone, or isoflavone backbones, even though it shares several common features with flavonoid-specific prenyltransferases.

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Induced biosynthesis of resveratrol and the prenylated stilbenoids arachidin-1 and arachidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage

Cited by 84 publications

References 39 publications

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Development of a liquid-liquid extraction method of resveratrol from cell culture media using solubility parameters

A Stilbenoid-Specific Prenyltransferase Utilizes Dimethylallyl Pyrophosphate from the Plastidic Terpenoid Pathway

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