Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance

Singh, S. D.; Rathore, Meenal; Goyary, Danswrang; Singh, Rupesh Kumar; Anandhan, Sivalingam; Sharma, Dinesh Kumar; Ahmed, Zakwan

doi:10.1007/s00299-011-1007-0

Cited by 39 publications

(35 citation statements)

References 34 publications

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“…It has also been shown that the overexpression of Arabidopsis AtCBF1 in tomatoes improves chilling tolerance (Singh et al 2011;Zhang et al 2011). In the present study, SlICE1 enhanced the expression of SlCBF1 and the cold-regulated SlDRCi7 gene ( Figure 3D), and promoted cold tolerance ( Figures 3A-C).…”

Section: Discussionmentioning

confidence: 48%

“…Many COR genes are regulated by CBF/DREB1 (C-repeat binding factor/ dehydrin responsive element binding protein 1) (JagloOttoson et al 1998;Liu et al 1998). e expression of Arabidopsis AtCBF1/DREB1B in tomato plants improves cold tolerance (Singh et al 2011) and induces several oxidative stress-responsive genes, such as CAT1 (which encodes catalase), to protect the plants from cold stress (Hsieh et al 2002). e overexpression of Arabidopsis AtCBF1/DREB1B in tobacco plants induces the activity of Cu/Zn superoxide dismutase (Yang et al 2010).…”

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confidence: 99%

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SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

Miura¹,

Shiba²,

Ohta³

et al. 2012

Plant Biotechnology

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Many abiotic and biotic stresses can reduce plant growth and development. Low temperature is one of the most harmful abiotic stresses, particularly for plants that are tropical or subtropical in origin. To elucidate the molecular mechanisms underlying the cold-stress response, components involved in the signal transduction of cold stress have been characterized. In this study, we characterized a basic helix-loop-helix (bHLH) transcription factor encoding gene, SlICE1, from tomato (Solanum lycopersicum), which shows similarity with Arabidopsis ICE1. e expression of SlICE1 was observed in younger leaves, owers, and green and red fruits. To characterize the function of SlICE1, overexpressing tomato lines were produced. SlICE1-overexpressing tomatoes exhibited chilling tolerance, and SlICE1 enhanced the expression of coldresponsive genes, such as SlCBF1 and SlDRCi7, as well as accumulation of ascorbic acid. e SlICE1 protein was degraded a er cold treatment. ese results indicate that SlICE1 enhances cold tolerance in tomatoes.

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Section: Discussionmentioning

confidence: 48%

mentioning

confidence: 99%

SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

Miura¹,

Shiba²,

Ohta³

et al. 2012

Plant Biotechnology

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“…Moreover, transgenic tomato plants contained higher levels of proline than wild-type plants under normal or water-deficient conditions. Singh et al (2011) obtained similar results by introducing into tomatoes the AT-CBF1 gene, which is driven by the inducible promoter RD29A (which contained several cis-acting elements, including DRE, ABRE). However, Singh et al (2011) did not observe any morphological disorders.…”

Section: Applicable Tomato Transformationmentioning

confidence: 56%

“…Singh et al (2011) obtained similar results by introducing into tomatoes the AT-CBF1 gene, which is driven by the inducible promoter RD29A (which contained several cis-acting elements, including DRE, ABRE). However, Singh et al (2011) did not observe any morphological disorders. The use of RD29A promoter instead of constitutive promoter in the tomatoes led to the development of cold-tolerant transgenic plants without any phenotypic abnormalities.…”

Section: Applicable Tomato Transformationmentioning

confidence: 56%

Tomato (Solanum lycopersicum L.) in the service of biotechnology

Gerszberg

Hnatuszko-Konka

Kowalczyk

et al. 2014

Plant Cell Tiss Organ Cult

168

110

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Originating in the Andes, the tomato (Solanum lycopersicum L.) was imported to Europe in the 16th century. At present, it is an important crop plant cultivated all over the world, and its production and consumption continue to increase. This popular vegetable is known as a major source of important nutrients including lycopene, bcarotene, flavonoids and vitamin C as well as hydroxycinnamic acid derivatives. Since the discovery that lycopene has anti-oxidative, anti-cancer properties, interest in tomatoes has grown rapidly. The development of genetic engineering tools and plant biotechnology has opened great opportunities for engineering tomato plants. This review presents examples of successful tissue culture and genetically modified tomatoes which resistance to a range of environmental stresses improved, along with fruit quality. Additionally, a successful molecular farming model was established.

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“…Measurement of relative water content (RWC) helps assessing the capability of a cell to tolerate the scarcity of water in its liquid form and has thus been tracked often as a parameter to study the capability of the cell to tolerate cold stress (Singh et al 2011). Proline accumulates in plant cells under a broad range of stress conditions and protects folded protein structures against denaturation.…”

Section: Introductionmentioning

confidence: 99%

Female plants of Hippophae salicifolia D. Don are more responsive to cold stress than male plants

Gupta

Grover

Pandey

et al. 2012

Physiol Mol Biol Plants

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Hippophae salicifolia D. Don (Seabuckthorn) grows in stressful environment of high altitude under conditions of low temperature and low availability of water. We have studied gender based differences in physiochemical response to cold stress in male and female plants of Seabuckthorn. After 24 h of cold stress about 32 and 66 % higher electrolyte leakage (EL) was recorded in male and female plants respectively. Relative water content (RWC) at the end of 24 h stress was higher in male plants (~64 %) compared to female plants (~60 %). Proline content in leaf samples of cold stressed male and female plants also increased upon cold stress. After 24 h about 2.7 fold higher amount of proline was assessed in male and female in comparison to control plants. Similarly, about two fold increase in the specific activities of superoxide dismutase (SOD) and catalase was also observed upon cold stress in male and female plants. These findings have important inferences for community of molecular biologists exploring seabuckthorn genome for agronomically important genes.

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Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance

Cited by 39 publications

References 34 publications

SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

Tomato (Solanum lycopersicum L.) in the service of biotechnology

Female plants of Hippophae salicifolia D. Don are more responsive to cold stress than male plants

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