Induced Pluripotent Stem Cells as a Cell-Based Therapeutic in Stroke

Hess, David C.; Fakhri, Nasir; West, Franklin D.

doi:10.1007/978-3-319-15063-5_9

Cited by 1 publication

(2 citation statements)

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“…12,48,49 We examined and compared the cell phenotype change of single cell dispersed hiPSC-NPCs in both soft and stiff Me-HA hydrogels in SDM for a period of 28 days. The encapsulated hiPSC-NPCs in the both hydrogel types expressed βIII-tubulin (green) and S100B (red) as shown after immunofluorescent staining, suggesting they spontaneously differentiated into mixed populations of cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…8,9 However, the inferior survival rate of grafted cells and inappropriate cell scaffold platform limit the efficacy of hiPSC-based regenerative therapies. 10-12 Therefore, development of physiologically relevant in vitro model with hiPSC-derived NPCs (hiPSC-NPCs) plays an important role in not only understanding neural network development, behavior and activities under physiological or pathological situations, but also identifying key characteristics of cell-matrix interactions to direct the appropriate design of functional grafts for CNS injuries or diseases. In addition, the cell fate of engrafted hiPSC-derived progenitor or precursor cells are typically poorly controlled, leading to the formation of teratomas, which is one of the major challenges in hiPSC-based cell therapies.…”

Section: Introductionmentioning

confidence: 99%

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Three-dimensional hyaluronic acid hydrogel-based models for in vitro human iPSC-derived NPC culture and differentiation

Duan

et al. 2017

J. Mater. Chem. B

102

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Human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) are considered as a promising cell source for transplantation and have been used for organoid fabrication to recapitulate central nervous system (CNS) diseases in vitro. The establishment of three-dimensional (3D) in vitro model with hiPSC-NPCs and control of their differentiation is significantly critical for understanding biological processes and CNS disease and regeneration. Here we implemented 3D methacrylated hyaluronic acid (Me-HA) hydrogels with encapsulation of hiPSC-NPCs as in vitro culture models and further investigated the role of the hydrogel rigidity on the cell behavior of hiPSC-NPCs. We first encapsulated single dispersive hiPSC-NPCs within both soft and stiff Me-HA hydrogel and found that hiPSC-NPCs gradually self-assembled and aggregated to form 3D spheroids. Then, the hiPSC-NPCs were laden into Me-HA hydrogels in the form of spheroids to evaluate their spontaneous differentiation in response to hydrogel rigidity. The soft Me-HA hydrogel-encapsulated hiPSC-NPCs displayed robust neurite outgrowth and showed high levels of spontaneous neural differentiation. We further encapsulated Down Syndrome (DS) patient-specific hiPSC-derived NPCs (DS-NPCs) spheroids within our hydrogels. DS-NPCs remained excellent cell viability in both soft and stiff Me-HA hydrogels. Similarly, soft hydrogels promoted neural differentiation of DS-NPCs by significantly upregulating neural maturation markers. This study demonstrates that soft matrix promotes neural differentiation of hiPSC-NPCs and HA-based hydrogels with hiPSC-NPCs or DS-NPCs are effective 3D models for CNS disease study.

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Section: Resultsmentioning

confidence: 99%