Induced Polyploidy in Arachis hypogaea L. var. Talam using Catharanthus roseus Phenolic Extract

Muarifin, Ahmad; Ilh, Ayudha Bahana; Sartika, Dian; Da, Budi Setiadi

doi:10.3923/ajps.2021.263.270

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…The morphological characters of stem length, leaf length, leaf width, and wet weight are higher and shows statistical difference to control. The phenotypic data obtained from this study are in line with three previous studies [9,10,18]. Meanwhile based on studies in peanut plants, any level of periwinkle extract application can boost the performance of peanut plants [10].…”

Section: Advances In Biological Sciences Research Volume 22supporting

confidence: 88%

“…The phenotypic data obtained from this study are in line with three previous studies [9,10,18]. Meanwhile based on studies in peanut plants, any level of periwinkle extract application can boost the performance of peanut plants [10]. The presence of vincristine and vinblastine compounds in periwinkle plants is assumed to have the ability to double the set of chromosomes in plants.…”

Section: Advances In Biological Sciences Research Volume 22supporting

confidence: 86%

“…Using 0.05% Bio-Catharantin with 8 hours incubation can doubled the chromosome number and increase the morphological size of habitus and fruit of melon cultivar Melodi Gama-1 [9]. The chromosome number of peanut was also doubled using 0.15% Bio-Catharantin with 24 hours of treatment [10]. Another Bio-Catharantin application on Citrus aurantiifolia and Citrus maxima cv.…”

Section: Introductionmentioning

confidence: 95%

“…As a result, collitetraploid plants are formed. Based on the previous research, Bio-Catharantin as periwinkle leaves extract, can increase the ploidy of melon (Cucumis melo L.) [9] and peanut (Arachis hypogaea L.) [10]. Using 0.05% Bio-Catharantin with 8 hours incubation can doubled the chromosome number and increase the morphological size of habitus and fruit of melon cultivar Melodi Gama-1 [9].…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Bio-Catharantin Induction to Increase Red Spinach (Alternanthera amoena Voss.) Production

Shafura,

Janah,

Huda

et al. 2022

Advances in Biological Sciences Research

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Red spinach (Alternanthera amoena Voss.) is a plant with high nutritional value and contains anthocyanin compounds that act as antioxidant compounds to prevent the formation of free radicals in the body, besides that anthocyanin can also treat anemia. The increase in demand for red spinach in the market has not been matched by the amount of production. The productivity of spinach can be increased by polyploid induction. The advantages of polyploid plants are increased quality (taste, aroma, and other metabolites), size of vegetative and generative structures, pest resistance, and more adaptability. Based on the latest research, it was found that Bio-Catharantin as a periwinkle leaf extract product can induce polyploidization in shallot (Allium cepa L.) and watermelon (Citrullus lanatus) plants. This study was carried out from July to August 2021. The treatments include control, seed induction by Bio-Catharantin 0.2% solution, and seed induction by Bio-Catharantin 0.4% solution for 12 hours, 24 hours, and 48 hours. The results showed that induction with Bio-Catharantin in plants was followed by changes in morphological characters. The results of ANOVA-Duncan analysis showed that there was a significant difference in seed induction by Bio-Catharantin 0.2% solution for 12 hours treatment. Chromosomal observations could not be identified because the chromosome size was not visible and hardly seen under the microscope. Flow cytometry test showed that plants treated with Bio-Catharantin were unable to produce polyploidization of red spinach. The result of the chlorophyll test is in line with the morphological characters.

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Section: Advances In Biological Sciences Research Volume 22supporting

confidence: 88%

Section: Advances In Biological Sciences Research Volume 22supporting

confidence: 86%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effectiveness of Bio-Catharantin Induction to Increase Red Spinach (Alternanthera amoena Voss.) Production

Shafura,

Janah,

Huda

et al. 2022

Advances in Biological Sciences Research

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Flow Cytometry Analysis of In Vitro Induced Polyploidy in Plants

Sudheer,

Banadka,

Nagella

et al. 2023

Genome Size and Genetic Homogeneity of Regenerated Plants: Methods and Applications

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Polyploidy is the condition of having more than two sets of chromosomes. The mechanism of polyploidy helps in deriving special traits like an increase in biomass, an increase in the size of various organ systems, and secondary metabolite content for the progeny. Various chemical compounds (colchicine, trifluralin, and oryzalin) that have the capacity to alter the mitotic cycle were used for the purpose of inducing polyploidy. Various techniques, such as counting of chromosome number, chloroplast number, determination of pollen diameter, and estimation of leaf stomatal density and size, were developed to analyze the polyploidy of the plants. However, these methods are not reliable for their regular use. Thus, of all the above-mentioned approaches, the estimation of ploidy level by flow cytometry (FCM) has been the most popular over the last few decades. Flow cytometry is now extensively used for the verification of haploidy, aneuploidy, and polyploidy. The ease of sample preparation, fast acquisition, and accurate measurements have made the method popular in the domains of plant cell biology, systematics, evolution, genetics, and biotechnology. The current chapter discusses the induction of polyploidy and its importance in plant breeding. It also emphasizes the importance of FCM in the analysis of polyploidy and enumerates the various polyploidy studies involving the application of FCM.

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Artificial Polyploid Plant Induction of Local North Sumatra Garlic (Allium sativum L.) with Oryzalin Treatment in M1 Generation

Sinuraya,

Rodiansah,

Hanafiah

2023

Asian J. of Plant Sciences

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Induced Polyploidy in Arachis hypogaea L. var. Talam using Catharanthus roseus Phenolic Extract

Cited by 3 publications

References 17 publications

Effectiveness of Bio-Catharantin Induction to Increase Red Spinach (Alternanthera amoena Voss.) Production

Effectiveness of Bio-Catharantin Induction to Increase Red Spinach (Alternanthera amoena Voss.) Production

Flow Cytometry Analysis of In Vitro Induced Polyploidy in Plants

Artificial Polyploid Plant Induction of Local North Sumatra Garlic (Allium sativum L.) with Oryzalin Treatment in M1 Generation

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