Abstract. Daryono BS, Subiastuti AS, Fatmadanni A, Sartika D. 2019. Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L. ‘Melonia’) based on ISSR markers. Biodiversitas 20: 1069-1075. Cucumis melo L. ‘Melonia’ was new Indonesian melon cultivars produced by segregation of Meloni cultivar. The Meloni cultivar has a yellowish-skinned color, orange flesh fruit, and sweet taste. The stability of phenotype and genotype characters of this new cultivars were assessed using 27 morphological traits and 4 ISSR primers, respectively. Phenotype characters in F2 and F3 populations have been stable on 25 of 27 morphological traits used, except in weight of seed cavity and color of skin fruit. The 4 ISSR markers were produced 41 fragments, contained 28 monomorphic DNA bands and 13 polymorphic DNA bands. All of DNA bands were scored and used for genetic similarity analysis using MVSP 3.1A Program. The highest genetic variation was produced by UBC-808 primer with a polymorphic percentage of 38.46%. All of ‘Melonia’ populations were clustered together with 100% similarity percentage.  Compared to other cultivars, ‘Melonia’ had high similarity with ‘Meloni’ in 87.8%, meanwhile, ‘Melonia’ and Meloni cultivars had relationship with Melona at a similarity value of 70.7%.

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Butternut squash (Cucurbita moschata) is a Cucurbitaceae plant that has been widely cultivated in Indonesia. Butternut squash is known to have various cultivars. A new cultivar introduced by the Faculty of Biology UGM is named ‘Citra Laga’ which is expected to be able to compete with the imported cultivars. The number of cultivars within a species may indicate genetic variation. This research was conducted to observe genetic variation and the phenetic relationship between ‘Citra Laga’ and the imported butternut squash cultivars based on the molecular marker ISSR. The ISSR analysis between ‘Citra Laga’ and the imported cultivars showed an average low polymorphism rate by 18.61% with a high similarity percentage of 83.7%. Thus, it can be said that the genetic variation is low and ‘Citra Laga’ is not genetically much different from the imported cultivars.

Viability and vigor are components of seed quality assessment which are influenced by internal and external factors of the seed, i.e., salinity and temperature. Salinity is related to the interaction between osmotic pressure and seed germination. This study used 3 types of seeds, they are peanuts (Arachis hypogaea L.), mung bean (Vigna radiata (L.) R. Wilczek), and corn (Zea mays L.). The purposes of this study were to determine seed viability by using Triphenyl tetrazolium chloride (TTC), to analyze the effects of different osmotic pressures and temperatures on seed viability and vigor. Peanut seed viability test was conducted by using 1% TTC solution. Testing the effect of osmotic pressure on seed viability and vigor was conducted by using various concentrations of NaCl (0.01 M; 0.05 M; 0.5 M; 0.1 M; 1 M). The germination test of the three seeds was conducted by using temperatures of 30°C and 35°C, each with three replications. The test parameters measured included germination, germination rate, germination speed index, vigor index, simultaneous growth, and the speed of seed growth. The results showed that TTC solution was effectively used to determine seed viability. Mung bean had the highest germination rate, LP, IKP, IV, simultaneous growth, and speed of seed growth compared to the other tested seeds in all salinity and temperature treatments. The 30 ⁰C temperature treatment resulted in the best viability and seed vigor for the three tested plants. Treatments of temperature and salinity affect the quality of the three plant seeds which can then be used to determine the quality of peanut, mung bean, and corn seeds.   Keywords: germination, salinity, triphenyl tetrazolium chloride, viability, vigour