Induced seed protein mutant of barley

Ingversen, John; Køie, B.; Doll, Hans

doi:10.1007/bf01946777

Cited by 117 publications

(28 citation statements)

References 3 publications

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“…Mutations affecting the overall lysine content of cereal endosperm have received considerable attention because lysine is the limiting essential amino acid in cereal grains when used as food for monogastric animáis (Mertz et al 1964;Nelson et al 1965;Munck et al 1970;Singh and Axtell 1973;Ingversen et al 1973;Dolí 1973Dolí , 1983. However, these so-called high-lysine mutations do not specifically affect lysine biosynthesis and their effect on lysine content is the indirect result of profound alterations in the pattern of protein accumulation throughout development and, at least in some cases, they also affect the metabolism of carbohydrates and lipids, as well as seed morphology.…”

Section: Introductionmentioning

confidence: 99%

“…While a major portion of the B hordein structural genes at the Hor-2 locus has been deleted in Ris0 56 (Kreis et al 1983), in the other two mutations, both of which map in sepárate loci of chromosome 7 (synonym 5H; Karlsson 1976Karlsson , 1977, trans-acting regulatory genes seem to be affected . The lys 3a mutation drastically reduces the net accumulation of all hordein polypeptides except D-hordein (Ingversen et al 1973;Hopp et al 1983;Thompson and Bartels 1983;Kreis et al 1984). The reduction in storage protein accumulation seems to bring about an increase in the synthesis of other polypeptides and in the level of free amino acids (Brandt 1976;Shewry et al 1980).…”

Section: Introductionmentioning

confidence: 99%

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The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Rodrı́guez-Palenzuela¹,

Royo²,

Gómez³

et al. 1989

Mol Gen Genet

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Summary.A cDNA encoding trypsin inhibitor CMe from barley endosperm has been cloned and characterized. The longest open reading frame of the cloned cDNA codes for a typical signal peptide of 24 residues followed by a sequence which is identical to the known amino acid sequence of the inhibitor, except for an lie/Leu substitution at position 59. Southern blot analysis of wheat-barley addition lines has shown that chromosome 3H of barley carries the gene for CMe. This protein is present at less than 2%-3% of the wild-type amount in the mature endosperm of the mutant Ris0 1508 with respect to Bomi barley, from which it has been derived, and the corresponding steady state levéis of the CMe mRNA are about 1%. One or two copies of the CMe gene (synonym Itcl) per haploid genome have been estimated both in the wild type and in the mutant, and DNA restriction patterns are identical in both stocks, so neither a change in copy number ñor a major rearrangement of the structural gene account for the markedly decreased expression. The mutation at the lys 3a locus in Ris0 1508 has been previously mapped in chromosome 7 (synonym 5H). A single dose of the wild-type alíele at this locus (Lys 3a) restores the expression of gene CMe (alíele CMe-1) in chromosome 3H to normal levéis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Rodrı́guez-Palenzuela¹,

Royo²,

Gómez³

et al. 1989

Mol Gen Genet

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“…The high-lysine genes usually decrease the synthesis of the lysine deficient storage protein, hordein (4,11,29,33). Hordein polypeptides are synthesized during endosperm development, deposited in protein bodies and may account for half of the total endosperm protein at seed maturity (7, Abbreviations: CM = Carlsberg Monoclonal; FITC = fluorescein isothiocyanate; lg = immunoglobulin; kD = kilodalton; MAb = monoclonal antibody; p = protein; PAGE = polyacrylamide gel electrophoresis; SDS = Sodium dodecyi sulphate; Tris = tris-(hydroxymethyl)-aminomethane.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibodies to hordein polypeptides

Ullrich

Rasmussen

Høyer‐Hansen

et al. 1986

Carlsberg Res. Commun.

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Monoclonal antibodies to hordein polypeptides have been produced and characterized. All antibodies reacted with more than one polypeptide, indicating that different hordein polypeptides have common epitopes. Of the seventeen isolated hybridoma lines, six secreted antibodies which reacted both with B-and C-hordein, five recognized only B-hordein and one secreted antibodies specific for C-hordein polypeptides. Two clones produced antibodies to an unidentified polypeptide doublet with an apparent molecular weight of 43 kD. These two polypeptides have at least one epitope in common with B-hordein polypeptides and are not extractable in water but in alcohol. They are not visible by Coomassie blue staining.The mutant M56 fails to synthesize B-hordein polypeptides due to a major deletion. It contains polypeptides in the B-hordein region of the gel, which have epitopes recognized by the B-hordein antibodies, but are different from B-hordeins. The 43 kD hordein doublet was more abundant in the mutant than in the wild-type endosperm, suggesting that these polypeptides like the C-hordein polypeptides are synthesized in larger amounts as a consequence of the absent B-hordein polypeptide synthesis. Mutant 1508 which has an impaired synthesis of both B-and C-hordein polypeptides was also found to be defective in the synthesis of the 43 kD polypeptide doublet. It was shown that immunofluoreseent techniques may be of practical importance to identify mutants with elevated or reduced ~tmounts of specific hordein polypeptides. Due to the higher sensitivity compared to SDS-PAGE, immunoblotting can be a useful tool for genotype identification in breeding programmes.

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“…A final viscosity analysis of seeds of M 4 plants led to the selection of four lines for detailed investigations. The M 5 plants from these four lines and the high lysine mutant Riso 1508 (18) were obtained from the Carlsberg Research Farm, Hyidagerg~rd, Denmark.…”

Section: Selection Of Mutantsmentioning

confidence: 99%

“…Since M-1460 also has enlarged embryos and shriveled endosperms, it is possible that M-1460 might be a high lysine mutant of the Riso-1508 type (18). This possibility was examined by comparing the amino acid composition of total seed protein from Minerva, M-737, M-1460 and Riso 1508 (Table II).…”

Section: 1chemical and Morphological Propertiesmentioning

confidence: 99%

Selection and characterization of low β-glucan mutants from barley

Aastrup

1983

Carlsberg Res. Commun.

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Four barley lines have been selected from 40,000 M 2 plants derived from sodium azide-treated seeds of the high [3-glucan cultivar Minerva, on the basis of low viscosity of acid extracts of flour. Two lines show a minor decrease in total ~-glucan content, and two lines are low in total [3-glucan, exhibit rapid endosperm modification and display a mealy endosperm. The thickness of the endosperm cell walls in the low [~-glucan mutants is 3.0-3.5/am compared with 6.5 lam for the parent variety Minerva. One of the low B-glucan mutants, has increased 13-glucanase activity which might partly explain the increased rate of its modification during malting. The other mutant, M-737, has approximately the same amount of [~-glucanase as Minerva and appears to be a genuine low [3-glucan mutant. Malting studies confirmed the important role of endosperm cell walls as a barrier for enzyme dissemination.

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Induced seed protein mutant of barley

Cited by 117 publications

References 3 publications

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Monoclonal antibodies to hordein polypeptides

Selection and characterization of low β-glucan mutants from barley

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