Induced sputum as a method for detection of systemic sclerosis-related interstitial lung disease

Yılmaz, Neslihan; Abul, Yasin; Bıçakçıgil, Müge; Golabi, Pegah; Çelikel, Çiğdem; Karakurt, Sait; Yavuz, Şule

doi:10.1007/s00296-011-1872-4

Cited by 8 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yilmaz et al 23 also reported an increase in mean macrophage and lymphocyte counts in the induced sputum of 20 subjects with systemic sclerosis compared with the control group, but unlike in other studies, including ours, they demonstrated a decreased number of neutrophils in the induced sputum of subjects with systemic sclerosis in comparison with the control group. In addition, in our study, we also found correlations between clinical and lung functional parameters and induced sputum findings in the total patient population and in those with restrictive PFT pattern.…”

Section: Discussioncontrasting

confidence: 77%

Induced Sputum Analysis in Subjects With Systemic Sclerosis

Litinsky¹,

Fireman

Paran³

et al. 2016

Respir Care

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 77%

Induced Sputum Analysis in Subjects With Systemic Sclerosis

Litinsky¹,

Fireman

Paran³

et al. 2016

Respir Care

View full text Add to dashboard Cite

show abstract

“…Progressive fibrosis in the skin and lungs are prominent, and ultimately leads to organ failure accounting for the substantial mortality of SSc [ 2 ]. Inflammatory infiltrates are observed in a variety of affected organs in early-stage disease [ 3 – 5 ] and are accompanied by elevated circulating levels of inflammatory cytokines and chemokines [ 6 , 7 ]. Serum amyloid A (SAA) is an evolutionarily conserved ˜ 12 kDa acute phase protein [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Serum Amyloid A Is a Marker for Pulmonary Involvement in Systemic Sclerosis

et al. 2015

View full text Add to dashboard Cite

Inflammation in systemic sclerosis (SSc) is a prominent, but incompletely characterized feature in early stages of the disease. The goal of these studies was to determine the circulating levels, clinical correlates and biological effects of the acute phase protein serum amyloid A (SAA), a marker of inflammation, in patients with SSc. Circulating levels of SAA were determined by multiplex assays in serum from 129 SSc patients and 98 healthy controls. Correlations between SAA levels and clinical and laboratory features of disease were analyzed. The effects of SAA on human pulmonary fibroblasts were studied ex vivo. Elevated levels of SAA were found in 25% of SSc patients, with the highest levels in those with early-stage disease and diffuse cutaneous involvement. Significant negative correlations of SAA were found with forced vital capacity and diffusion capacity for carbon monoxide. Patients with elevated SAA had greater dyspnea and more frequent interstitial lung disease, and had worse scores on patient-reported outcome measures. Incubation with recombinant SAA induced dose-dependent stimulation of IL-6 and IL-8 in normal lung fibroblasts in culture. Serum levels of the inflammatory marker SAA are elevated in patients with early diffuse cutaneous SSc, and correlate with pulmonary involvement. In lung fibroblasts, SAA acts as a direct stimulus for increased cytokine production. These findings suggest that systemic inflammation in SSc may be linked to lung involvement and SAA could serve as a potential biomarker for this complication.

show abstract

“…Damjanov et al [26] studying the IS of 68 SSc-ILD patients (versus 18 HV) observed a significant increase in total number of inflammatory cells, of neutrophils, lymphocytes and eosinophils, and lower macrophages, in SSc patients. Yialmaz et al [27] studying the IS of 17 SSc-ILD patients (versus 12 non-ILD pulmonary controls) observed a significant increase in total number of inflammatory cells, of lymphocytes and macrophages, and lower neutrophils, in SSc patients. Litinsky et al [25] studying the IS of 20 SSc-patients (versus 16 HV) reported an isolated decrease in lymphocytes (p=0.045), with no change in the percentage of neutrophils, macrophages and eosinophils.…”

Section: Discussionmentioning

confidence: 99%

“…The cell suspension was then centrifuged again at 400 g at 4 □C for 10 min. Squamous cells, total cell counts and cell viability checked by trypan blue exclusion were performed with a manual hemocytometer [27]. The differential leukocyte count was obtained using a cytospin stained with Rapi Diff II (Atom Scientific, Manchester UK) on 500 non squamous cells.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, induced sputum (IS), which is less invasive and of clinical interest for airway diseases such as idiopathic pulmonary fibrosis (IPF)[21-24], can be proposed as a good non-invasive tool to study lung inflammation in patients with SSc. Previous researches using IS and BALF in SSc have mainly focused on the cellularity [25-27]. If many biomarkers have already been studied both in serum and BALF from ILD patients and in particular in IPF [23,28], very few works have been done in SSc as recently reviewed [29].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory profile of induced sputum composition in systemic sclerosis: a comparison with healthy volunteers

Jacquerie

Henket

Andre

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundSystemic sclerosis (SSc) is a potentially serious and disabling connective tissue disease specially in case of interstitial lung disease (SSc-ILD). The aim of our study was to evaluate the potential utility of dosing in the induced sputum (IS) and to compare their levels in SSc-ILD and SSc-nonILD patients, as well as in healthy volunteers (HV). IS and sera values were also compared.MethodsIn a prospective cross-sectional analysis, we studied the IS and serum provided from 25 SSc patients, 15 SSc-nonILD and 10 SSc-ILD, compared to 25 HV. We analyzed sputum cell composition and quantified in the supernatant and corresponding serum by commercially available immunoassays: IGFBP-1, IGFBP-2, IGFBP-3, TGF-β, IL-8, TNF-α, YKL-40, MMP-7 and MMP-9. Lung function was studied by the determination of FEV-1 (%), FVC (%), DLCO (%) and KCO (%).ResultsThe IS of SSc patients had a lower weight than HV (p<0.01) without any significant difference with regard to the cellularity. IGFBP-1 (p<0.0001), TGF-β (p<0.05), IL-8 (p<0.05), YKL-40 (p<0.0001) and MMP-7 (p<0.01) levels were increased in the IS of SSc patients compared to HV. Only IL-8 serum levels (p<0.001) were increased in SSc patients compared to HV. Neither in IS nor in serum were observed differences between SSc-ILD and SSc-nonILD patients. Correlations were observed between IS IL-8 levels and FEV-1 (%)(r=-0.53, p<0.01), FVC (%) (r=-0.51, p<0.01) and annualized □KCO (%) (r=0.57, p<0.05), between IS TGF-□ levels and annualized □FEV-1 (%) (r=-0.57, p<0.05), between IS IGFBP-2 levels and annualized □KCO (%) (r=0.56, p<0.05).ConclusionOur study showed that SSc patients exhibit raised IS levels of IGFBP-1, TGF-β, IL-8, YKL-40 and MMP-7, molecules known to be involved in lung remodeling and fibrotic process, without any significant difference between SSc-ILD and SSc-nonILD patients. IL-8, TGF-□ and IGFBP-2 are correlated with lung function in SSc patients which emphasize clinical relevance. IS analysis represents a new approach to understand lung inflammatory process in SSc patients. A longitudinal study is needed to evaluate their pathophysiological relevance.

show abstract

Induced sputum as a method for detection of systemic sclerosis-related interstitial lung disease

Cited by 8 publications

References 21 publications

Induced Sputum Analysis in Subjects With Systemic Sclerosis

Induced Sputum Analysis in Subjects With Systemic Sclerosis

Serum Amyloid A Is a Marker for Pulmonary Involvement in Systemic Sclerosis

Inflammatory profile of induced sputum composition in systemic sclerosis: a comparison with healthy volunteers

Contact Info

Product

Resources

About