2015
DOI: 10.1094/mpmi-05-15-0102-r
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Inducible Expression of Agrobacterium Virulence Gene VirE2 for Stringent Regulation of T-DNA Transfer in Plant Transient Expression Systems

Abstract: Agrotransfection with viral vectors is an effective solution for the transient production of valuable proteins in plants grown in contained facilities. Transfection methods suitable for field applications are desirable for the production of high-volume products and for the transient molecular reprogramming of plants. The use of genetically modified (GM) Agrobacterium strains for plant transfections faces substantial biosafety issues. The environmental biosafety of GM Agrobacterium strains could be improved by … Show more

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Cited by 18 publications
(15 citation statements)
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“…The inducible system was cloned from the plasmids pAJM.336 (LacI), pAJM.661 (TtgR), pAJM.677 (AraE), pAJM.771 (NahR), and pAJM.773 (VanR), respectively (Meyer et al, 2019). The CymR inducible system and Pcym (Ptac/cuo) promoter were synthesized via gblock by IDT according to the sequences described by Denkovskienė et al (2015). Then, these fragments were assembled to generate the plasmids pBBR1‐B2, pBBR1‐B4, pBBR1‐B5, pBBR1‐B7, pBBR1‐B9, and pBBR1‐B11.…”
Section: Methodsmentioning
confidence: 99%
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“…The inducible system was cloned from the plasmids pAJM.336 (LacI), pAJM.661 (TtgR), pAJM.677 (AraE), pAJM.771 (NahR), and pAJM.773 (VanR), respectively (Meyer et al, 2019). The CymR inducible system and Pcym (Ptac/cuo) promoter were synthesized via gblock by IDT according to the sequences described by Denkovskienė et al (2015). Then, these fragments were assembled to generate the plasmids pBBR1‐B2, pBBR1‐B4, pBBR1‐B5, pBBR1‐B7, pBBR1‐B9, and pBBR1‐B11.…”
Section: Methodsmentioning
confidence: 99%
“…The best example is the development of the binary vector that opens the door for DNA transformation (Anand et al, 2018). An expression system based on T7 RNA polymerase was tested; however, its function remains to be further investigated due to contractionary results from previous reports (Denkovskienė et al, 2015; Kang et al, 2007). Additionally, several inducible systems were developed, which include arabinose‐ and Isopropyl ß‐D‐1‐thiogalactopyranoside (IPTG)‐controlled systems to regulate gene expression (Newman & Fuqua, 1999), a tetracycline‐inducible system to control the expression of the lambda Red recombination system and Cre‐recombinase (Hu et al, 2014; Liu et al, 2016), and a cumic acid‐inducible system to regulate virE2 expression (Denkovskienė et al, 2015).…”
Section: Introductionmentioning
confidence: 99%
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“…To construct pSRKKM-P cym , a synthesized gBlock from IDT Integrated DNA Technologies was made containing the regulatory elements of the cumate system similar to previously described plasmid constructs [76, 77]. The P cym promoter region is annotated in Table S4.2.…”
Section: Methodsmentioning
confidence: 99%